Recombinant Human KGF (FGF-7), Animal-free
Keratinocyte Growth Factor (KGF) is one of 23 known members of the FGF family, and it is known as FGF-7. Proteins of this family play a central role during prenatal development, postnatal growth, and the regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. KGF is a mitogen factor specific for epithelial cells and keratinocytes. Recombinant Human KGF is an 18.9 kDa protein consisting of 163 amino acid residues.
Features:
- Secretion System
- No Endotoxin
- Less Derivatives (impurities)
- Experience: Food Amino Acid Production
Product Details
| Product Name: | Recombinant Human KGF (FGF-7) |
|---|---|
| Source: | Corynebacterium glutamicum* |
| Formulation: | Frozen in DPBS(-) containing 1.5mM DTT and 100mM Na2SO4 |
| Quality: | Animal origin free |
| AA Sequence: | CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT |
| Purity: | ≥ 95% by SDS-PAGE |
| Endotoxin | <0.1EU/ug |
Product Comparison Table
| AJINOMOTO | Company A | Company B | |
| Host cell | C. glutamicum | E. coli | E. coli |
| Endotoxin | <0.1EU/μg (LAL) |
<1EU/μg (LAL) |
<0.10EU/μg (LAL) |
| Purity | ≧95% (SDS-PAGE) |
≧95% (SDS-PAGE、HPLC) |
>97% (SDS-PAGE) |
| Biological activity | EC50, 0.1-30 ng/mL*1 | ED50, ≦10 ng/ml*2 | ED50, 6-60 ng/ml*3 |
| Form | Frozen in DPBS(-) containing 1.5 mM DTT, 100 mM Na2SO4 |
Lyophilized | Lyophilized |
| N-terminal | Cys-Asn | Met-Cys-Asn | Met-Cys-Asn |
| Expiration date | 2 years from manufacturing date |
At least 4 years from manufacturing date |
12 months from date of receipt |
- *1 ERK1/2 phosphorylation assay using MCF-7 cells
- *2 Dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors
- *3 Cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells
Comparison of biological activity with other companies’ products
Phosphorylation of ERK1/2
AJINOMOTO’s KGF demonstrates equal biological activity compared to tested alternatives!
Stability information after reconstitution
| AJINOMOTO | Company A | Company B | |
| -80°C | 2 years from manufacturing date |
3 months from reconstitution date |
3 months from reconstitution date |
| Freeze-thaw cycles |
Within 3 times | ー | ー |
Product Information
Recombinant Human KGF (FGF-7), Animal-free (Product Information)Ordering Information
Shipping with dry ice within the US (Shipping Temp.: -20C)