Recombinant Human SCF, Animal-free
SCF (stem cell factor) is a growth factor secreted from fibroblasts and endothelial cells. SCF binds to a receptor known as c-Kit and is thought to play an important role in the survival, proliferation and differentiation of hematopoietic stem cells. Recombinant Human SCF is an 18.5 kDa polypeptide containing 164 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.
Features:
- Secretion System
- No Endotoxin
- Less Derivatives (impurities)
- Experience: Food Amino Acid Production
Product Details
| Product Name: | Recombinant Human SCF |
|---|---|
| Source: | Corynebacterium glutamicum* |
| Formulation: | Frozen in 20 mM HEPES |
| Quality: | Animal origin free |
| AA Sequence: | EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA |
| Purity: | ≥ 95% by SDS-PAGE |
| Endotoxin: | <0.1EU/ug |
Product Comparison Table
| AJINOMOTO | Company A | Company B | Company C | |
| Host cell | C. glutamicum | E. coli | CHO | Human |
| Endotoxin | <0.1EU/μg (LAL) |
<1EU/μg (LAL) |
<1.0EU/μg (LAL) |
<1EU/μg (LAL) |
| Purity | ≧95% (SDS-PAGE) |
≧98% (SDS-PAGE、HPLC) |
>97% (SDS-PAGE) |
>97% (SDS-PAGE) |
| Biological activity |
EC50, 1.0-20 ng/mL*1 | ED50, ≦2.0 ng/ml*2 | ED50, ≦1-5 ng/ml*2 | ED50, ≦5 ng/ml*2 |
| Form | Frozen (in 20mM HEPES) |
Lyophilized | Lyophilized | Lyophilized |
| N-terminal | Glu-Gly-Ile | Met-Glu-Gly | Met-Glu-Gly | Met-Glu-Gly |
| Expiration date | 2 years from manufacturing date |
At least 4 years from manufacturing date |
12 months from date of receipt |
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- *1 Phosphorylation assay of c-KIT
- *2 Cell proliferation assay using TF‑1 human erythroleukemic cells
Comparison of biological activity with other companies’ products
Phosphorylation assay of c-KIT
AJINOMOTO’s SCF demonstrates equal to biological activity compared to tested alternatives!
Product Information
Recombinant Human SCF, Animal-free (Product Information)Stability information after reconstitution
| AJINOMOTO | Company A | Company B | |
| -80°C | 2 years from manufacturing date |
1 year from reconstitution date |
3 months from reconstitution date |
| 4°C | At least 1 month | 1 month from reconstitution date |
1 month from reconstitution date |
| Freeze-thaw cycles | Within 3 times | ー | ー |
Ordering Information
Shipping with dry ice within the US (Shipping Temp.: -20C)