Versican G1 domain / for Immunohistochemistry and ELISA
Hyaluronan Bindling Protein (HABP), Versican G1 domain, bind specifically to Hyaluronan (HA) and doesn’t bind to other glycosaminoglycan and DNA, it can be used for HA detection probe. The applicatipon below shows localization of HA in the 3D skin model was assessed by histology with Biotinylated HA Binding Protein (2 µg/ml).
|No Hyaluronidase treatment||Hyaluronidase Treatment|
|Source :||Escherichia Coli.|
|Buffer :||Phosphatase buffered saline pH 7.4, 10% Glycerol|
|Description :||Versican G1 domain bind specifically to hyaluronan and doesn’t bind to other glycosaminoglycan, DNA. Versican G1 domain can use probe for hyaluronan detection.|
|Characterization :||On SDS-PAGE commassie blue stained gel,the purified recombinant protein showes a band at 36.7kDa.Purity is greater than 95% by SDS-PAGE gel.|
For detection of the hyaluronan (HA)
The optimal dilution for specific application should be determined by the investigator.
|Storage :||Store at -20 to -80. Avoid freeze/thaw cycles.|
Tengblad, A. "A comparative study of the binding of cartilage link protein and the hyaluronate-binding region of the cartilage proteoglycan to hyaluronate-substituted Sepharose gel." Biochemical Journal 199.2 (1981): 297-305.
Tengblad, Anders. "Quantitative analysis of hyaluronate in nanogram amounts." Biochemical Journal 185.1 (1980): 101.
Mubuchi, Ayumu, et al. "Assembly of neuron-and radial glial cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons." bioRxiv (2022): 2022-09.
Rizzo, Giulia, et al. "Dysfunctional Extracellular Matrix Remodeling Supports Perianal Fistulizing Crohn′ s Disease by a Mechanoregulated Activation of the Epithelial-to-Mesenchymal Transition." Cellular and Molecular Gastroenterology and Hepatology (2022).
Momoeda, Masahiro, et al. "Deletion of hyaluronan-binding protein involved in hyaluronan depolymerization (HYBID) results in attenuation of osteoarthritis in mice." The American Journal of Pathology 191.11 (2021): 1986-1998.
Bistoletti, Michela, et al. "Involvement of hyaluronan in the adaptive changes of the rat small intestine neuromuscular function after ischemia/reperfusion injury." Scientific reports 10.1 (2020): 1-17.