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Biotinylated Hyaluronan Binding Protein (HABP), Recombinant

Versican G1 domain / for Immunohistochemistry and ELISA

Hyaluronan Bindling Protein (HABP), Versican G1 domain, bind specifically to Hyaluronan (HA) and doesn’t bind to other glycosaminoglycan and DNA, it can  be used for HA detection probe. The applicatipon below shows localization of HA in the 3D skin model was assessed by histology with Biotinylated HA Binding Protein (2 µg/ml).

No Hyaluronidase treatment  Hyaluronidase Treatment


Source :  Escherichia Coli.
Buffer :  Phosphatase buffered saline pH 7.4, 10% Glycerol
Description :  Versican G1 domain bind specifically to hyaluronan and doesn’t bind to other glycosaminoglycan, DNA. Versican G1 domain can use probe for hyaluronan detection.
Characterization : On SDS-PAGE commassie blue stained gel,the purified recombinant protein showes a band at 36.7kDa.Purity is greater than 95% by SDS-PAGE gel.
Application : 
For detection of the hyaluronan (HA)
Immunohistochemistry, ELISA
The optimal dilution for specific application should be determined by the investigator.
Storage :  Store at -20 to -80. Avoid freeze/thaw cycles.
Reference : 

Tengblad, A. "A comparative study of the binding of cartilage link protein and the hyaluronate-binding region of the cartilage proteoglycan to hyaluronate-substituted Sepharose gel." Biochemical Journal 199.2 (1981): 297-305.

Tengblad, Anders. "Quantitative analysis of hyaluronate in nanogram amounts." Biochemical Journal 185.1 (1980): 101.

Ordering Information

Product Size Storage Cat.No. PKG Size Price  
Biotin-HABP (Recombinant); Biotinylated Hyaluronan Binding Protein 200 ul/vial (0.25 mg/ml) -20 BC41 50 µg 416.00 Buy
HABP (Recombinant); Hyaluronan Binding Protein 200 ul/vial (0.25 mg/ml) -20 BC40 50 µg 208.00 Buy