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Centrifugal Filtration Plates for 96-well and 384-well

Shorter time and easier! / Mesh-equipped microplate

For folow cytometer users, this is a mesh-equipped microplate in which specimens are dispensed by multichannel pipette, automatic dispenser, etc. with stacked on another microplate, then filtrated at one time by centrifuge. This is convenient for those who want to operate more easily and with less time.

  • Mesh size: 30 µm, 70 µm, 100 µm

  • Material: Polystylene (Plate), Polyester fiber (Mesh), Silicone (Adhesive)

  • Compatible with 96 and 384 well plate

     

How to Use

 (1) Use stacked on your microplate (2) Sample is dispensed into each well with using multichannel pipette or automatic dispenser (3) Filtration with centrifuge *Recommended: 300g x 30 seconds

Performance evaluation for 96 well centrifugal filtration plate

Evaluation method

Stack centrifugal filtration plate on conventional 96 well round bottom plate, then cell filtration by centrifuge.

Evaluation point: Presence or absence of contamination after centrifugation

HEK293T (human fetal kidney epithelial cells) without expressing GFP and HEK293T with expressing GFP are filtrated in adjacent wells and judged the presence or absence of contamination by flow cytometry.

 

Protocol
1. Peel cells with trypsin/EDTA and centrifuge
2. Wash cells with PBS
3. Dilute cells with PBS to about 2 x 104 cells/200 µL
4. Add PI to final concentration of 0.25 µg/mL
5. Stack centrifugal filtration plate on 96 well plate
6. Dispense cells with 200 µL each to wells
7. Centrifuge the plate at 300 g for 30 seconds
8. Measure by flow cytometer (Guava easyCyte 8HT)
*Measured only within the red frame

Dispensing cells in the plate

 

Centrifugal filtration plate & 96 well plate after centrifugation

 
Evaluation result

 

1. According to the above graphs, no observation of contamination in the adjacent wells.

2. All cell suspension fell to the 96 well plate with centrifugation at 300 g for 30 seconds.

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Centrifugal filtration plate for 96-well, 30 µm R-CSC078-02 RT 10 plates 572.00 Buy
Centrifugal filtration plate for 96-well, 30 µm R-CSC078-03 RT 50 plates 2,643.00 Buy
Centrifugal filtration plate for 96-well, 70 µm R-CSC079-02 RT 10 plates 572.00 Buy
Centrifugal filtration plate for 96-well, 70 µm R-CSC079-03 RT 50 plates 2,643.00 Buy
Centrifugal filtration plate for 96-well, 100 µm R-CSC080-02 RT 10 plates 572.00 Buy
Centrifugal filtration plate for 96-well, 100 µm R-CSC080-03 RT 50 plates 2,643.00 Buy
Centrifugal filtration plate for 384-well, 30 µm R-CSC0783-02 RT 10 plates 858.00 Buy
Centrifugal filtration plate for 384-well, 70 µm R-CSC0793-02 RT 10 plates 858.00 Buy
Centrifugal filtration plate for 384-well, 100 µm R-CSC0803-02 RT 10 plates 858.00 Buy