BioMasher - Disposable Homogenizer
BioMasher- Innovative disposable homogenizer
The patented BioMasher, a high-performance disposable homogenizer, is suitable for extracting proteins, RNA, DNA, and other molecules from animal and plant tissues. BioMasher is available in a wide variety of sizes and hardnesses for a wide range of research and analytical applications.
BioMasher Lineup
| BioMasher I | BioMasher II | BioMasher III | BioMasher V | BioMasher sp | PowerMasher II | |
|---|---|---|---|---|---|---|
|
Feature
|
Many prep at a time
|
For high yield
|
For high yield
Many prep at a time
|
For high yield
Many prep at a time
|
For high yield
| High torque to support crushing |
| Collection Amount | Small | Large | Large | Large | Large | N/A |
| Producing Heat | Heatless | Manageable | Manageable | Manageable | Manageable | Manageable |
| Required Device | Centrifuge | None | Centrifuge | Centrifuge | None | II, III, V, SP |
| Soft Tissues | Excellent | Excellent | Excellent | Excellent | Excellent | N/A |
| Hard Tissues | Fair | Excellent | Excellent | Excellent | Excellent | N/A |
BioMasher I
BioMasher I : Efficient and easy sample crushing by centrifugal separation.
Two types are available- with O-rings to prevent sample backflow and without O-rings for easy crushing.
disrupted. The fibrous membrane component of the sample will be caught by the filter. The pestle has a cross-shaped blade at the tip. By pushing and turning the pestle manually, the sample, which is hard to disrupt by centrifugation alone, can be ground.
BioMasher I with an O-ring is suitable for homogenizing soft tissue with centrifugal force alone. In particular, it is suitable for simultaneous processing of many samples and for infectious samples.
Without an O-ring
Hard tissue may not be homogenized sufficiently by centrifugal force alone. The model without an O-ring is suitable when grinding the sample on the filter area before centrifugation to enhance disruption efficiency.
* DO NOT add the buffer in the filter tube before centrifuge. Otherwise the filter may be clogged, and the tube may be damaged. For only samples with lots of fibrous components with little water content, such as epidermis, mesentery, tail etc., add a small amount of buffer to extract and filtrate the target molecules.
BioMasher II
BioMasher II
- Homogenizer with innter dimpled tube and surface dimpled pestle for effective sample crushing
- Pestle prevents samples scattering and backflow
- Capable of crushing various types of samples (animal tissues, organs, plants and insects)
BioMasher II consists of a 1.5 mL microtube and a pestle. The sample and extraction buffer are placed in the tube, and ground to prepare the homogenate. The microtube and pestle are molded so that they fit without any space between them. The texture of the inner wall of the tube and the outer periphery of the tip of the pestle enhances the efficiency of the disruption of the sample. The pestle has a splash guard, which is designed to fit the tube without unnecessary spaces. BioMasher II can be used to disrupt various samples, such as animal tissue/organ, plants (stems, roots, seeds), and insects.
• For samples that are difficult to disrupt, an exclusive electric stirrer (Nippi PowerMasher II) is available for easy disruption.
• DO NOT autoclave BioMasher II. In the case sterilization is required, please use a sterilized product.
BioMasher SP (Large capacity type of BioMasher II)
BioMasher SP- Large capacity type of BioMasher II
BioMasher SP is a larger volume version of BioMasher II. This model has enhanced disruption efficacy from the combination of the 15 mL tube with an abrasive inner wall and a stirring pestle with a textured tip. The shovel-like shape cutout and the helical groove of the pestle effectively trap and homogenize the tissue. BioMasher SP is suitable for disruption of animal tissue/organ, plants (stems, roots, seeds), and insects.
• For samples difficult to disrupt, an exclusive electric stirrer (Nippi PowerMasher II) is available for easy disruption.
• DO NOT autoclave BioMasher II. In the case sterilization is required, please use a sterilized product.
BioMasher III
BioMasher III : Double crushing effect by grinding and filtering
- Combining the features of the BioMasher I and II
- Crushes, centrifuges and filters samples using a tube with dimpled inner surface and an agitator with dimpled surface
- Ideal for crushing samples with high fiber content
BioMasher III has the features of both BioMasher I and II. This model consists of a filter tube with an abrasive surface inner wall and a pestle with a textured surface. After grinding the sample in the filter tube using the pestle, centrifuge the extraction liquid. The extracted sample will be filtered and caught in the tube. Since the fibrous membrane component get trapped by the filter, BioMasher III is recommended for homogenization of highly fibrous samples.
• For samples that are difficult to homogenize, an exclusive electric stirrer (Nippi PowerMasher II) can be used for ease.
BioMasher V (Larger capacity type of BioMasher III )
BioMasher V: Larger capacity type of BioMasher III
BioMasher V is a larger volume version of BioMasher III. After homogenizing the sample with a combination of the tube with a textured inner wall and the stirring pestle with an abrasive surface, centrifuge and filtrate with the filter. Recommended for homogenizing highly fibrous samples.
• For samples difficult to homogenize, an exclusive electric stirrer (PowerMasher II) is available for ease.
PowerMasher II - Pestle motor for BioMasher II, III, V and SP
PowerMasher II is a handy battery powered grinder designed for BioMasher II, III, V, and SP. It can be set on the stirring rod of a BioMasher and stirred electrically. The motor speed is 450 rpm. Two AA batteries are included.
Biomasher Users ManualFAQ
General
| Question | Answer | |
|
General |
In terms of sample volume, which model of the BioMasher is appropriate? | Our recommendation is as below. Sample size BM I: 50 mg BM II: 100 mg BM III: 50 mg BM V: 500 mg BM SP: 1,000 mg |
| General | What is the centrifugation resistance of the BioMasher? | Although it depends on sample, Please try the following conditions as a standard. BM I: 10,000-15,000 g, 10-30 seconds BM II: 10,000-15,000 g, 10-30 seconds BM III: 6,000-10,000 g, 10-30 seconds BM V: 3,000-6,000 g, 5 minutes BM SP: 3,000-6,000 g, 5 minutes |
| General | What is the lowest temperature BioMasher can bear? | The material of the tube is PP, and its freezing resistance is approximately -60°C to -40°C. Cooling of the sample with liquid nitrogen together with the tube may cause damage. |
| General | How to extract DNA from different tissue samples with BioMasher series? | Please see the "User's Manual" P8-9 |
| General | Do the sterilized items come with a certificate, such as a sterilization certificate? | We have a sterilization certificate, so please feel free to request it. |
| General | Do BioMasher series have resistance to phenol-based reagents? | The following conditions may result from phenol-based reagents of BioMasher SP, for example. +20℃: No change +60℃: Possibility of cracks, strength degradations, and discolorations |
| General | Can BioMasher series be used for grinding platelets? The volume of the platelet suspension is approximately 2 ml. |
If the filter pore size is larger than the material to be disrupted, efficient disruption is likely to be difficult. Both PP and PE filters have sizes larger than platelets, so it is expected that BioMasher series are not suitable for platelet disruption. |
| General | Are the products RNase free even if they are not sterilized? | We do not perform RNase tests for finished products, so DNase free and RNase free are not guaranteed. Since each factory conducts hygiene management from a number of perspectives, including the flow of goods and human hygiene, DNase or RNase contaminations have not been reported. For customers who require a precondition for DNase free and RNase free, we suggest adding additional clearance processes before using our products. |
| Biomasher I and III | What are the filter sizes of BioMasherⅠand III? |
1）Catalog No. 893012 (1.5ml tube with O-ring & PP filter): Pore size 80～145μｍ
2）Catalog No. 893041 (1.5ml tube with O-ring & PE filter): Pore size 20～60μm
3）Catalog No. 893131 (PP filter of BioMasher III): Pore size 80～145μｍ
For more information, please see the "User's Manual" P1-5 |
| Biomasher II and III | For hard tissue such as tumor, are any preparations required before using BioMasher Ⅱ or BioMasher Ⅲ? | Using samples that have been pre-crush is expected to enable more reliable disruption. Since the Biomassher III is equipped with a filter, fibrous tissues such as tumor tissue may remain on the filter. The use of the Biomassher II is recommended. |
| BioMasher II | What are the efficiencies of BioMasher Ⅱ for protein extraction? | Compared with conventional SSI pestle, extraction is more effective with BioMasher Ⅱ. Please see the "Technical Document" |
| BioMasher II | Can "Biomassher II" be cooled with liquid nitrogen? | The material of the tube is PP, and its freezing resistance is approximately -60℃ ~ -40℃. Cooling of the sample with liquid nitrogen together with the tube may cause damage. If you want to avoid an increase in sample temperature, freeze the material to be homogenized first, then homogenize it on ice. |
| BioMasher II | Are individual parts, such as 1.5ml tubes or pestles for BioMasher II available to purchase? | Individual parts sales are not available. |
| BioMasher II | Can BioMasher II be used for extracting lipid metabolite from an animal organ tissue samples? | After grinding the sample with BioMasher Ⅱ, add a hydrophobic solvent and centrifuge it. This may allow for the recovery of lipid metabolites. |
| BioMasher II | It is stated that homogenizing cultured cells with the "Biomassher II" is not very suitable. What is the reason for this? | Cultured cells are smaller than the grinding surfaces of the BioMasher II, so there is a possibility that they may not be completely homogenized. |
| BIomasher III and V | What are the filter materials of BioMasher Ⅲ and BioMasher Ⅴ, and what are their hydrophilicity? | The materials of both are polyethylene, and the hydrophilicity is not high. |
| PowerMasher II | What is an optimal protocol of the PowerMasher II, such as rotation speed, time, and strength? | The rotation speed of the PowerMasher II is set to 450 rpm, which is relatively low, and as a result, the rotational torque is increased. This allows the rotation to continue even when the material inside the tube is pressed, making it efficient for disrupting hard samples. The optimal time and strength vary depending on the material being grinded, so we recommend applying for a one-month rental of the PowerMasher II and testing the grinding using BioMasher samples at the same time. |
| PowerMasher II | Can PowerMasher Ⅱ be used for all models of BioMasher series? | PowerMasher II can be used for BioMasher Ⅱ, Ⅲ, Ⅴ, SP（cannot be used for BioMasher I）. |
Ordering Information
BioMasher
| Product | Storage | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| BioMasher I (1.5 ml Microtube, O-ring, PE-filter) | Room Temp. | 893041 | 30 sets | 57.00 | Buy |
| BioMasher I (1.5 ml Microtube, O-ring, PP-filter) | Room Temp. | 893012 | 30 sets | 57.00 | Buy |
| BioMasher I (1.5 ml Microtube, Without O-ring, PP-filter) | Room Temp. | 893011 | 30 sets | 51.00 | Buy |
| BioMasher II (EOG-sterilized), 100 sets | Room Temp. | 893062 | 200 sets (2 x 100 sets) | 310.00 | Buy |
| BioMasher II (Non-sterilized), 100 sets | Room Temp. | 893061 | 200 sets (2 x 100 sets) | 257.00 | Buy |
| BioMasher III (Non-sterilized), 50 sets | Room Temp. | 893131 | 100 sets (2 x 50 sets) | 184.00 | Buy |
| BioMasher V (EOG-sterilized), 50 sets | Room Temp. | 893153 | 100 sets (2 x 50 sets) | 277.00 | Buy |
| BioMasher sp (EOG-sterilized), 40 sets | Room Temp. | 893163 | 80 sets (2 x 40 sets) | 277.00 | Buy |
PowerMasher
| Product | Storage | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| PowerMasher II | Room Temp. | 893002 | 1 unit | - | Inquire |
- The shipping method is FedEx Ground or equivalent service.