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EZSPHERE™ for 3D cell culture with microwells

Cell Culture Ware for Spheroid Formation

Three dimensional (3D) cell culture systems have gained in popularity as invaluable tools in broad applications of cell biology. 3D multi-cellular cell aggregates (Spheroid) can be formed by using a low attachment culture surface. However, variability in forming spheroids has been a persistent problem. EZSPHERE is specifically designed to form a large number of uniformly sized Spheroids and Embryoid Bodies (EBs).

 

 

 

Features

  • The surface of EZSPHERE is coated with very low binding 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer.
  • EZSPHERE has a lot of evenly designed micro wells on the surface -- 6 different well size available. 
    Micro well type

     Diameter (µm) 

     Depth (µm)

     

    #901 250 100
    #900 400~600 100~300
    #902 450 200
    #903 950 400
    #904 1,000 350
    #905 1,600 800

Unique micro-fabricated plastic vessel

Useful for mass-production of EBs with uniform size

EB formation, proliferation and differentiation protocols

High efficient generation of EBs with uniform size

EB-size control with micro-well sizes or inoculating cell densities

(A) Micro-well size and topography of two different types of EZSPHERE

 

(B) EB sizes affected by the micro-well size and/or cell density

Two types of EZSPHERE #900 and #905 with micro-wells about 400-600 µm and 1,600 µm in diameter, respectively, were used to analysis the effect of the micro-well size or inoculated cell density on the EB sizes (A). Inoculation of iPSCs as 400 or 2,000 cells per micro-well on the same type of EZSPHERE #900 resulted in the formation of EBs with different sizes, while inoculating iPSCs as 9,000 cells per micro-well on the another EZSPHERE #905 resulted larger size of EBs (B). (Scale bars: 400 µm)

Proliferation of iPSCs with maintaining pluripotency

Induction of dopaminergic neurons from the EBs

Induction of cardiomyocyte from the EBs

Referred protocol / References

Referred protocol: 

iPS spheroid formation in EZSPHERETM devices

Visit manufacture's website for more detail: http://www.atgc.co.jp/div/rika/hbin/ez2012.html

 

 

References: 

1. Zhang RR, Takebe T, Miyazaki L, Takayama M, Koike H, Kimura M, Enomura M, Zheng YW, Sekine K, Taniguchi H
Efficient Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Three-Dimensional Microscale Culture
Stem Cells and Tissue Repair Methods in Molecular Biology Volume 1210, 2014, pp 131-141
 
2. Shimada H, Hashimoto Y, Nakada A, Shigeno K, Nakamura T,
Accelerated generation of human induced pluripotent stem cells with retroviral transduction and chemical inhibitors under physiological hypoxia
Biochemical and Biophysical Research Communications, Volume 417, Issue 2, Pages 659-664
 
3. Aikawa N, Suzuki Y, Takaba K
A Simple Protocol for the Myocardial Differentiation of Human iPS Cells
Biological and Pharmaceutical Bulletin Vol. 38 (2015) No. 7 Pages 1070-1075
 
4. Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T
TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues
Scientific Reports 6, Article number: 21747 (2016) doi:10.1038/srep21747
 
5. Hiroki Sato, Alimjan Idiris, Tatsuaki Miwa, Hiromichi Kumagai
Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
Scientific Reports 6, Article number: 31063 (2016) doi:10.1038/srep31063
 
6. Taku Nakayama, Shimpei Otsuka, Tatsuya Kobayashi, Hodaka Okajima, Kentaro Matsumoto, Yuichiro Hagiya, Keiji Inoue Taro Shuin, Motowo Nakajima, Tohru Tanaka, Shun-ichiro Ogura
Dormant cancer cells accumulate high protoporphyrin IX levels and are sensitive to 5-aminolevulinic acid-based photodynamic therapy
Scientific Reports 6, Article number: 36478 (2016)doi:10.1038/srep36478
 
7. Ayako Aihara, Natsuki Abe, Koichiro Saruhashi, Tatsuro Kanaki, Taito Nishino
Novel 3-D cell culture system for in vitro evaluation of anticancer drugs under anchorage-independent conditions
Cancer ScienceVolume 107, Issue 12 December 2016 Pages 1858−1866 DOI: 10.1111/cas.13095
 
8. Waseem K. Raja, Alison E. Mungenast, Yuan-Ta Lin, Tak Ko, Fatema Abdurrob, Jinsoo Seo, Li-Huei Tsai
Self-Organizing 3D Human Neural Tissue Derived from Induced Pluripotent Stem Cells Recapitulate AlzheimerÕs Disease Phenotypes
PLOS ONE 11(9): e0161969. doi: 10.1371/journal.pone.0161969
 
9. Hiroyuki Koike, Ran-Ran Zhang, Yasuharu Ueno, Keisuke Sekine, Yun-Wen Zheng, Takanori Takebe, Hideki Taniguchi
Development 2017 144: 1018-1024; doi: 10.1242/dev.143032
 
10. Sugimura, R., Jha, D. K., Han, A., Soria-Valles, C., Da Rocha, E. L., Lu, Y. F., ... & Wong, I. (2017).
Nature545(7655), 432-438.
 
11. Matsuoka Lab @Northwestern University:  Rachel A Heuer et al. (2020).
Tissue Eng Part A., Online ahead of print.
 
12. McMahon Lab @University of Southern California:  Tracy Tran et al. (2022).
Cell Stem Cell, 29(7):1083-1101.e7. 
 
13. Allard Lab @UCLA:  Roxane Verdikt et al. (2023).
bioRxiv, Online ahead of print. « « NEW!
 
14. Ota Lab @The University of Chicago:  Yojiro Koda et al. (2023).
Ann Thorac Surg Short Rep, Online ahead of print. « « NEW!

Ordering Information

#900 Diameter: 500µm, Depth: 100µm
Product Cat.No. Size Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish 4000-900SP No. of Well: approx. 2,700/dish Room Temp. 10 dishes 430.00 Buy
EZSPHERE™ 60 mm Dish 4010-900SP No. of Well: approx. 6,500/dish Room Temp. 10 dishes 460.00 Buy
EZSPHERE™ 100 mm Dish 4020-900SP No. of Well: approx. 17,000/dish Room Temp. 10 dishes 490.00 Buy
EZSPHERE™ 6-well Plate 4810-900SP No. of Well: approx. 2,700/well Room Temp. 5 plates 265.00 Buy
EZSPHERE™ 24-well Plate 4820-900SP No. of Well: approx. 470/well Room Temp. 5 plates 265.00 Buy
EZSPHERE™ 96-well Plate 4860-900SP No. of Well: approx. 100/well Room Temp. 5 plates 265.00 Buy
#901 Diameter: 200µm, Depth: 100µm
Product Cat.No. Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish TCI-4000-901SP Room Temp. 10 dishes - Inquire
#902 Diameter: 500µm, Depth: 200µm
Product Cat.No. Size Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish 4000-902SP No. of Well: approx. 2,700/dish Room Temp. 10 dishes - Inquire
#903 Diameter: 800µm, Depth: 400µm
Product Cat.No. Size Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish 4000-903SP No. of Well: approx. 1,300/dish Room Temp. 10 dishes 440.00 Buy
EZSPHERE™ 6-well Plate TCI-4810-903SP Room Temp. 5 plates - Inquire
EZSPHERE™ 12-well Plate TCI-4815-903SP-10P Room Temp. 10 plates - Inquire
EZSPHERE™ 12-well Plate TCI-4815-903SP-50P Room Temp. 50 plates - Inquire
EZSPHERE™ 96-well Plate TCI-4860-903SP-10P Room Temp. 10 plates - Inquire
#904
Product Cat.No. Size Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish 4000-904SP No. of Well: approx. 700/dish Room Temp. 10 dishes - Inquire
EZSPHERE™ 6-well Plate TCI-4810-904SP Room Temp. 5 plates - Inquire
#905 Diameter: 1,400µm, Depth: 600µm
Product Cat.No. Size Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish 4000-905SP No. of Well: approx. 260/dish Room Temp. 10 dishes 440.00 Buy

The shipping method is FedEx Ground or equivalent service.