EZVIEW™ Glass Bottom Culture Plates / EZVIEW™ Glass Bottom Assay Plates
Culture Plate LB / Assay Plate
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By using cover glass with a controlled thickness of 0.15–0.2 mm, these plates provide clearer images at higher magnifications compared to conventional microplates.
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The low-base design brings the cover-glass surface closer to the microscope stage (approximately 0.6 mm distance), ensuring compatibility with a wide range of microscope models and objective lenses.
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The black-sided plate construction makes the plates suitable for fluorescence-based measurements and observations.
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EZVIEW Culture Plates are available in 6-well, 24-well, and 96-well formats. They are sterilized with ethylene oxide gas (EOG).
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EZVIEW Assay Plates are available in the 96-well format and are supplied non-sterilized
Imaging of Multicolor Immunofluorescence Staining with Confocal Microscope System
Product: EZVIEW™ Glass Bottom Culture Plates, 96-well
Cell Type: HeLa cells (human cervix uteri cancer)
Immunostaining: Nucleus with DAPI (blue), Plasma membrane with CellMask Deep Red (purple), Golgi apparatus with Alexa Fluor 568 (green)
Microscope: Nicon Inverted Microscope Systems Ti-E and Nicon Confocal Microscope System A1+
Magnification: 40X
Numerical Aperture: 0.95
Data provided by Nicon
Notes
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Do not use solvents such as methanol or ethanol, as they may cause the adhesive to peel.
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The glass bottom cannot be removed.
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Cell adhesion may differ from that of standard polystyrene culture vessels.
Reference
Aida, Rieko, Kazunori Okano, Kyoko Nakata, and Hiromi Hagiwara. "Apigenin-induced Apoptosis in Lung Adenocarcinoma A549 Cells: Involvement in IFNA2, TNF, and SPON2 With Different Time Points." Anticancer Research 45, no. 7 (2025): 2781-2790.
Aung, Winn, Atsushi B. Tsuji, Zhao-Hui Jin, Aya Sugyo, Chie Kajiwara, and Tatsuya Higashi. "Comparative Evaluation of Wavelength-Dependent Photodynamic Therapy Efficacy Using Representative Red and Near-Infrared Photosensitizers in a Single Tumor Model." Technology in Cancer Research & Treatment 24 (2025): 15330338251390292.
Fujita, Shunsuke, Tomotaka Shibata, Haruto Nishida, Sawa Matsumoto, Shusaku Kurogi, Shinji Yano, Shoichi Fumoto et al. "Establishment of a novel organoid line from esophageal squamous cell carcinoma with cytoplasmic vacuoles: Association of autolysosome swelling with vacuole formation." Pathobiology (2025).
Yoon, Jeongwoong, Taro Tsuda, Enrico Bortoletto, Akari Sakaguchi, Mutsuko Kobayashi, Umberto Rosani, Hayato Yokoi, Makoto Osada, Paola Venier, and Kazue Nagasawa. "Simple Transgene Overexpression using Scallop Hemocyte Culture Platform Enables Functional Genetic Research in Molluscs." Marine Biotechnology 27, no. 3 (2025): 94.
--- Reference ---
(1) Kobayashi J, Hasegawa T, Sugeno N, Yoshida S, Akiyama T, Fujimori K, Hatakeyama H, Miki Y, Tomiyama A, Kawata Y, Fukuda M, Kawahata I, Yamakuni T, Ezura M, Kikuchi A, Baba T, Takeda A, Kanzaki M, Wakabayashi K, Okano H, Aoki M,
Extracellular α-synuclein enters dopaminergic cells by modulating flotillin-1–assisted dopamine transporter endocytosis
The FASEB JOURNAL Published:18 Jun 2019
https://doi.org/10.1096/fj.201802051R
The FASEB JOURNAL Published:18 Jun 2019
https://doi.org/10.1096/fj.201802051R
(2) Kawano S, Ito k, Yahata K, Kira K, Abe T, Akagi, T, Asano M, Iso K, Sato Y, Matsuura F, Ohashi I,
A landmark in drug discovery based on complex natural product synthesis
Scientific Reports, Published: 17 June 2019
https://doi.org/10.1038/s41598-019-45001-9
Scientific Reports, Published: 17 June 2019
https://doi.org/10.1038/s41598-019-45001-9
(3) Janet Tan S, Nakahara K, Sou K, Takeoka S,
An Assay to Evaluate the Function of Liposomal Platelet Substitutes Delivered to Platelet Aggregates
Front. Bioeng. Biotechnol, Published: 12 April 2019,
https://doi.org/10.3389/fbioe.2019.00077
Front. Bioeng. Biotechnol, Published: 12 April 2019,
https://doi.org/10.3389/fbioe.2019.00077
(4) Mashima K, Takahashi S, Minami K, Izawa Y, Abe T, Tsukada N, Hishiki T, Suematsu M, Kajimura M, Suzuki N,
Neuroprotective Role of Astroglia in Parkinson Disease by Reducing Oxidative Stress Through Dopamine-Induced Activation of Pentose-Phosphate Pathway
SAGE journals, Published May 16, 2018,
https://doi.org/10.1177/1759091418775562
SAGE journals, Published May 16, 2018,
https://doi.org/10.1177/1759091418775562
(5) Aoki M, Das D, Hayashi H, Aoki-Ogata H, Takamatsu Y, Arun K. Ghosh, Mitsuya H
Mechanism of Darunavir (DRV)’s High Genetic Barrier to HIV-1 Resistance: A Key V32I Substitution in Protease Rarely Occurs, but Once It Occurs, It Predisposes HIV-1 To Develop DRV Resistance
ASM Journals, Published 6 March 2018,
DOI: 10.1128/mBio.02425-17
ASM Journals, Published 6 March 2018,
DOI: 10.1128/mBio.02425-17
(6) Usui K, Mie M, Andou T, Mihara H, Kobatake E
Fluorescent and luminescent fusion proteins for analyses of amyloid beta peptide aggregation
Journal of Peptide Science Volume 23, Issue 7-8 July-August
2017 Pages 659–665
Journal of Peptide Science Volume 23, Issue 7-8 July-August
2017 Pages 659–665
(7) Mon H, Lee JM, Sato M, Kusakabe T
Identification and functional analysis of outer kinetochore genes in the holocentric insect Bombyx mori
Insect Biochemistry and Molecular Biology Volume 86, July 2017, Pages 1-8 https://doi.org/10.1016/j.ibmb.2017.04.005
Insect Biochemistry and Molecular Biology Volume 86, July 2017, Pages 1-8 https://doi.org/10.1016/j.ibmb.2017.04.005
(8) Aoki M, Hayashi H, Rao KV, Das D, Higashi-Kuwata N, Bulut H, Aoki-Ogata H, Takamatsu Y, Yedidi RS, Davis DA, Hattori S, Nishida N, Hasegawa K, Takamune N, Nyalapatla PR, Osswald HL, Jono H, Saito H, Yarchoan R, Misumi S, Ghosh AK, Mitsuya H
A novel central nervous system-penetrating protease inhibitor overcomes human immunodeficiency virus 1 resistance with unprecedented aM to pM potency
eLife. 2017; 6: e28020. doi: 10.7554/eLife.28020
eLife. 2017; 6: e28020. doi: 10.7554/eLife.28020
(9) Nakamura T, Campbell JR, Moore AR, Otsu S, Aikawa H, Tamamura H, Mitsuya H
Development and validation of a cell-based assay system to assess human immunodeficiency virus type 1 integrase multimerization
Journal of Virological Methods Volume 236, October 2016, Pages 196-206 https://doi.org/10.1016/j.jviromet.2016.07.023
Journal of Virological Methods Volume 236, October 2016, Pages 196-206 https://doi.org/10.1016/j.jviromet.2016.07.023
(10) Aoki M, Hayashi H, Yedidi RS, Martyr CD, Takamatsu Y, Aoki-Ogata H, Nakamura T, Nakata H, Das D, Yamagata Y, Ghosh AK, Mitsuya H
C-5-Modified Tetrahydropyrano-Tetrahydofuran-Derived Protease Inhibitors (PIs) Exert Potent Inhibition of the Replication of HIV-1 Variants Highly Resistant to Various PIs, including Darunavir
Journal of Virology March 2016 vol. 90 no. 5 2180-2194
Journal of Virology March 2016 vol. 90 no. 5 2180-2194
(11) Iizumi T, Takahashi S, Mashima K, Minami K, Izawa Y, Abe T, Hishiki T, Suematsu M, Kajimura M, Suzuki N
A possible role of microglia-derived nitric oxide by lipopolysaccharide in activation of astroglial pentose-phosphate pathway via the Keap1/Nrf2 system
Journal of Neuroinflammation 201613:99
https://doi.org/10.1186/s12974-016-0564-0
Journal of Neuroinflammation 201613:99
https://doi.org/10.1186/s12974-016-0564-0
(12) Ando J, Asanuma M, Dodo K, Yamakoshi H, Kawata S, Fujita K, Sodeoka M
Alkyne-Tag SERS Screening and Identification of Small-Molecule-Binding Sites in Protein
Journal of the American Chemical Society 2016,
138 (42), pp 13901–13910 DOI: 10.1021/jacs.6b06003
Journal of the American Chemical Society 2016,
138 (42), pp 13901–13910 DOI: 10.1021/jacs.6b06003
(13) Naito H, Goshima G
NACK Kinesin Is Required for Metaphase Chromosome Alignment and Cytokinesis in the Moss Physcomitrella Patens
Cell Structure and Function Vol. 40 (2015) No. 1 p. 31-41
Cell Structure and Function Vol. 40 (2015) No. 1 p. 31-41
(14) Takahashi H, Yanagisawa T, Kim KS, Yokoyama S, Ohnishi M
Multiple Functions of Glutamate Uptake via Meningococcal GltT-GltM l-Glutamate ABC Transporter in Neisseria meningitidis Internalization into Human Brain Microvascular Endothelial Cells
Infect. Immun. September 2015 vol. 83 no. 9, 3555-3567
Infect. Immun. September 2015 vol. 83 no. 9, 3555-3567
(15) Nakagawa T, Ohta K, Kubozono K, Ishida Y, Naruse T, Takechi M, Kamata N
Zoledronate inhibits receptor activator of nuclear factor kappa-B ligand-induced osteoclast differentiation via suppression of expression of nuclear factor of activated T-cell c1 and carbonic anhydrase 2
Archives of Oral Biology Volume 60, Issue 4, April 2015,
Pages 557–565
Keywords: Bisphosphonate, Zoledronate, DNA microarray,
RANKL, NFATc1, CAII
Archives of Oral Biology Volume 60, Issue 4, April 2015,
Pages 557–565
Keywords: Bisphosphonate, Zoledronate, DNA microarray,
RANKL, NFATc1, CAII
(16) Edzuka T, Yamada L, Kanamaru K, Sawada H, Goshima G
Identification of the Augmin Complex in the Filamentous Fungus Aspergillus nidulans
PLoS ONE 9(7): e101471. doi:10.1371/journal.pone.0101471(2014)
PLoS ONE 9(7): e101471. doi:10.1371/journal.pone.0101471(2014)
(17) Chowdhury MM, Danoy M, Rahman F, Shinohara M, Kaneda S, Shiba K, Fujita N, Fujii T, Sakai Y
Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo
BioMed Research International Volume 2014 (2014), Article
ID 241571, 13 pages
BioMed Research International Volume 2014 (2014), Article
ID 241571, 13 pages
Ordering Information
| Product | Description | Storage | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|---|
| EZVIEW™ Glass Bottom Culture Plates, 6-well | Sterilized with ethylene oxide gas (EOG) | Room Temp. | 5816-006 | 10 | 308.00 | Buy |
| EZVIEW™ Glass Bottom Culture Plates, 24-well | Sterilized with ethylene oxide gas (EOG) | Room Temp. | 5826-024 | 10 | 319.00 | Buy |
| EZVIEW™ Glass Bottom Culture Plates, 96-well | Sterilized with ethylene oxide gas (EOG) | Room Temp. | 5866-096 | 10 | 330.00 | Buy |
| EZVIEW™ Glass Bottom Assay Plate, 96-well | Non-sterilized | Room Temp. | 5882-096 | 10 | 286.00 | Buy |
The shipping method is FedEx Ground or equivalent service.