DNA Synthesis Services (OGAB™)
Precision, long-chain & complex DNA (from a few kb to over 100 kb) / Remarkable efficiency. No compromises.
OGAB™ (Ordered Gene Assembly in Bacillus subtilis) by Synplogen is our proprietary DNA fragment assembly method that combines biotechnology and digital technology. This method is an excellent technique for synthesizing DNA of various lengths from a few kb to over 100 kb, high/low GC content, containing repeat sequences, and other complex sequences by linking more than 50 DNA fragments. We have established a production process consisting of (1) design of optimal DNA fragments for assembly (OGAB block) using our proprietary software, (2) synthesis and assembly of OGAB block using automated equipment, and (3) circularization and cloning of DNA utilizing the characteristics of B. subtilis, and succeeded in commercial production of synthetic DNA by the OGAB™ for the first time in the world.
Features:
- Up to 150kb Ultra Long Gene
- Extreme high GC or AT
- Repeat sequence, Tandem repeats
- Lethal sequence for E. coli
Advantages of Using Bacillus Subtilis
OGAB™ is the only technology in the world that uses B. subtilis for DNA synthesis. B. subtilis differs significantly from Escherichia coli in its DNA cleavage at the cell surface, the incorporation of linear DNA into the cell, and the spontaneous circularization of DNA within the cell. The greatest advantage of OGAB™ is that it avoids the in vitro plasmid DNA circularization reaction, which is a challenge with E. coli. This has made it possible to synthesize long-strand DNA, which was difficult in the past. Moreover, endotoxin-free DNA required for medical applications can also be synthesized.
Patent: No. 6440636, No. 6692873, No. 6926270
OGAB™ Synthesis Results
OGAB™ has a proven track record of synthesizing over 500 types of DNA. At Synplogen, we have successfully synthesized almost all of the DNA that we have been commissioned to synthesize.
Customer Testimonials
- Professor, National University
“Synplogen's high-accuracy synthesis of our large DNA fragments was key to generating our next-generation mouse models. Their work enabled our success.”
- Researcher, Food & Pharma R&D
“Synplogen succeeded where others couldn‘t. We handed them our most difficult synthesis projects—ones other companies turned down. They delivered 100% of them on time, keeping our critical research and development on track. We consider them essential to our success.”
- Researcher, Chemical R&D
“Synplogen delivers what others can‘t: long, repetitive DNA, with 100% on-time delivery. Their quality and predictability are a massive advantage for our R&D planning. They are our go-to for complex synthesis.”
- Professor, Leading Private University
"We selected Synplogen for a simple reason: they were the only ones who could do the job. Our JST CREST project required synthesis of long-chain (>10kb) AI-designed DNA and subsequent assembly in Bacillus subtilis. No other company, domestically or globally, has this expertise. Their team handled our complex requirements with exceptional speed and precision.”
- Researcher , Food & Pharma R&D
"Multiple vendors told us our long, GC-rich repeat sequence was impossible to make. Synplogen proved them wrong. They delivered a perfect product that was critical for our experiments, and we can't thank them enough. They are now our go-to partner for any sequence that others deem 'unsynthesizable’.”
References
Tsuge, Kenji, Yukari Sato, Yuka Kobayashi, Maiko Gondo, Masako Hasebe, Takashi Togashi, Masaru Tomita, and Mitsuhiro Itaya. "Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments." Scientific reports 5, no. 1 (2015): 10655.
