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Reversed phase chromatography / Open colomun chromatography

Open column chromatography is an excellent and easy technique for large scale preparation and purification at low cost. Reversed phase C18 packing materials are used but restricted to about 30-50% water in the mobile phase. The COSMOSIL C18-OPN is a new "Water-Wet" C18 packing material developed for Reversed Phase Open Column Chromatography. The C18-OPN material can be used in 100% aqueous eluents.





C18 packing materials in 100% water Normal C18 materials float on water

Surface of the C18-OPN Gel

The external surface of the C18-OPN gel is coated with hydrophilic groups.

Influence of Particle Size

Effect of Particle Size

Effect of particle size
Particle (um) Flow rate (ml/min) Theoretical plate number Rs Separation time(min) Consumption of solvent (ml)
75 0.25 400 390 340 1.00 1.74 240 60
140 0.60 300 280 260 0.90 1.40 100 60

Separation of Hydrophilic Compounds in Aqueous Solution

In reversed phase chromatography, hydrophilic compounds such as Theobromine and Theophylline could be separated under low concentration of organic solvent. The figure shows they are clearly separated by reversed open column chromatography with 70% of water.

Separation of p-Cresol and p-Ethylphenol by normal and reversed phase mode

Since the structural difference between p-Cresol and p-Ethylphenol is only one methyene group. It is difficult to separate such samples under normal phase condition. On the other hand, the samples are clearly separated under reversed phase condition with COSMOSIL C18-OPN packing material.


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  3. Miyazaki, Sho, et al. "Identification of a cyanidin-3-O-β-galactoside in gall tissue induced by midges on Japanese beech (Fagus crenata Blume)." Bioscience, Biotechnology, and Biochemistry 84.4 (2020): 797-799.
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  7. Ito, Masahiro, et al. "Isolation and identification of the antimicrobial substance included in tempeh using Rhizopus stolonifer NBRC 30816 for fermentation." International Journal of Food Microbiology (2020): 108645.
  8. Nakashima, Ken-ichi, et al. "Absolute configurations of talaromycones A and B, α-diversonolic ester, and aspergillusone B from endophytic Talaromyces sp. ECN211." Beilstein Journal of Organic Chemistry 16.1 (2020): 290-296.
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Ordering Information

Product Cat.No. PKG Size Price(US$)  
COSMOSIL 75C18-OPN 37842-66 100G 242.00 Buy
COSMOSIL 75C18-OPN 37842-95 500G 924.00 Buy
COSMOSIL 75C18-OPN 37842-11 1KG 1,628.00 Buy
COSMOSIL 140C18-OPN 37878-16 100G 242.00 Buy
COSMOSIL 140C18-OPN 37878-45 500G 924.00 Buy
COSMOSIL 140C18-OPN 37878-61 1KG 1,796.00 Buy