High consistency: Enables consistent cell differentiation

Free of undefined components (Serum, Human-derived component). Defined composition minimizes lot-to-lot variation and enables consistent cell differentiation.

High Safety: Animal-origin free composition

Free of animal- and human-derived components.
Animal-origin free composition minimizes risk of immunogenic contamination.

HPC differentiation with StemFit For Differentiation

hiPSCs (201B7, 1210B2) were seeded with StemFit Basic04CT in the presence of 10 μM Y-27632 on VTN-N coated 6-well plate at 1 x 106 cells/well on day 1. Cells were fed with each medium on day 0, 1, 2 and replated on day 3 with Medium 4 on Prime Surface at 1.5 x 104 cells/well. Expression of HE marker CD31, CD34 were eveluated on day 3 and ezpression of HPC maker CD43, CD45 wereevaluated on day 10 by FACS. The graph show average HE and HPC yield on day 3 and 10.

Reference

1. Loh MK, et al. WO2016141084

2. Referred from Zhu, H, et al. 2019

StemFit For Differentiation is useful for lineage-specific differentiation in replacement of serum-free supplement.

StemFit For Differentiation can support spontaneous differentiation of hiPSCs via EB formation.

StemFit For Differentiation is provided frozen and should be stored at below -20 °C until use. Use sterile techniques to prepare differentiation medium as follow.

1. Before use, thaw the frozen StemFit For Differentiation with occasionally mixing at room temperature (15-25 °C) or overnight at 4°C.
   CAUTION: Do not thaw StemFit for Diff. at 37 °C, as it accelerates the degradation of medium
2. Add 100 mL of StemFit For Diff. (5X) to 400 mL basal medium (DMEM/F12、RPMI1640、DMEM etc.) and mix thoroughly to make differentiation basic medium. If precipitations are observed, keep the bottle at room temperature and dissolve them.
   Optional: StemFit For Diff. and differentiation basic medium may be aseptically aliquoted and stored at below -20 °C. Once thawed, they may be stored at 2-8 °C for up to 2 weeks (Do not re-freeze.). We recommend storing the medium protected from light.
3. To differentiate each lineage, induction factors or cytokines can be added as specified by differentiation protocols.
4. Before use, warm aliquots to room temperature and use immediately.

Q: Which basal medium can be used with StemFit For Differentiation?

A: Stemfit For Differentiation can be used with any Basal medium (DMEM, RPIM1640, DMEM/F12) which are used for iPS/ES cell differentiation  

Q: What is the recommended final concentration of Stemfit For Differentiation?

A: We usually recommend final concentration of 20%. For example, mix 100 ml of StemFit For Differentiation with 400 ml of basal medium to prepare differentiation basic medium. Lineage specific cytokines or small molecules should be added to differentiation basic medium.

Q: Can I use StemFit For Differentiation for all lineage?

A: We have confirmed that StemFit For Differentiation can be used for EB formation and directed differentiation for endodermal, mesodermal and ectodermal lineages. Please refer the product information. There are some cases where protocol optimization would be required. Please contact us directly for more information.

・Product Information & Protocol (PDF [460KB])

・Hematopoietic progenitor cell differentiation protocol (PDF [483KB])

・Publications Using StemFit™ For Differentiation (PDF [465KB])

・SDS (PDF [56KB])

・GMP statement (PDF [581KB])