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Anti-GFP (Rat IgG2a), Monoclonal (GF090R)

Features

  • Immunohistochemical application
  • Rat monoclonal antibody

Anti-GFP (Rat IgG2a), Monoclonal (GF090R)

Clone : GF090R
Isotype : IgG2a (Rat)
Product form : Liquid
Immunogen : His-GFP (full length) fusion protein
Application : Immunohistochemistry 1:1000-1:2000
Western Blotting 1:1000-1:2000
ELISA 1:2000-1:20000

Anti-GFP (Rat IgG2a), Monoclonal (GF090R), Agarose Conjugate

Antibody protein : ca. 2 mg antibody / ml agarose
Product form : 1:1 suspension in Dulbecco's PBS(-)

Western Blotting

Sample : 1. GFP, 2. GFP-14-3-3 (293 cell lysate 50 µg)
Gel : 5-20% Gradient gel
Primary antibody : Anti-GFP(Rat IgG2a), Monoclonal (3 µg/ml), O/N
Secondary antibody : Anti-Rat IgG-HRP (1:10000)

Data courtesy of Dr. K. Yoshida, Depertment of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University.

Immunoprecipitation : Anti-GFP (Rat IgG2a), Agarose Conjugate

Protocol

  1. Add Anti-GFP (Rat IgG2a), agarose conjugate (20 µl) in 293 Cell Lysate (1 ml) expressed EGFP or EGFP-myosin (1ml each). Incubate at 4°C for 1 hour.
  2. Add SDS Sample buffer. After heating the samples at 90°C for 10 minutes, load to SDS-PAGE.
  3. Detect EGFP and EGFP-myosin using Anti-GFP (Rat IgG2a).

Data courtesy of Prof. A. Kakizuka, Laboratory of Functional Biology, Kyoto University Graduate School of Biostudies.

Reference
  1. Kumeta, M. et al. J Cell Sci. 123(Pt 7):1020-30 (2010)

Immunocytochemistry

Sample : Mouse brain (nerve cell)
Primary antibody : Anti-GFP(Rat IgG2a), Monoclonal (1:1000), RT, O/N
Secondary antibody : Anti-Rat IgG-Cy3 (1:300), RT, 1hr
Blocking : 5% Normal goat serum/0.2% TritonX-100 in PBS
Fixing method : 4% Paraformaldehyde

Data courtesy of Dr. Y. Yoshihara, RIKEN Brain Science Institute

Reference

  1. Yi, Peishan, and Gohta Goshima. "Rho of plants GTPases and cytoskeletal elements control nuclear positioning and asymmetric cell division during Physcomitrella patens branching." Current Biology 30.14 (2020): 2860-2868.

  2. Zhang, Wanzhen, et al. "Redox-Sensitive Cysteines Confer Proximal Control of the Molecular Crowding Barrier in the Nuclear Pore." Cell Reports 33.11 (2020): 108484.

  3. Liu, Can, et al. "Posterior subthalamic nucleus (PSTh) mediates innate fear-associated hypothermia in mice." Nature Communications 12.1 (2021): 1-15.

  4. Nakamura, Yoshiaki, et al. "Transient suppression of transplanted spermatogonial stem cell differentiation restores fertility in mice." Cell stem cell (2021).

  5. Uemura, Masato, et al. "A neurogenic microenvironment defined by excitatory-inhibitory neuronal circuits in adult dentate gyrus." Cell Reports 36.1 (2021): 109324.

  6. Yasui, Hiroyuki, et al. "Global knockdown of retinoid-related orphan receptor α in mature Purkinje cells reveals aberrant cerebellar phenotypes of spinocerebellar ataxia." Neuroscience (2020).

  7. Huerta-Ocampo, Icnelia, et al. "Distribution of midbrain cholinergic axons in the thalamus." eNeuro 7.1 (2020).

  8. Akiba, Masumi, et al. "Dopamine modulates the optomotor response to unreliable visual stimuli in Drosophila melanogaster." European Journal of Neuroscience 51.3 (2020): 822-839.

  9. Kim, Hyunsoo, et al. "Wiring patterns from auditory sensory neurons to the escape and song‐relay pathways in fruit flies." Journal of Comparative Neurology (2020).

  10. Tian, Xueying, et al. "Generation of a self‐cleaved inducible Cre recombinase for efficient temporal genetic manipulation." The EMBO Journal (2020).

  11. Yonehara, K. Nature. 469(7330):407-10 (2011)

  12. Abe, K. et al. Biol Reprod 85(5):1013-24 (2011)

  13. Hide, T. et al. Stem Cells. 29(4):590-9 (2011)

  14. Kurita, S. et al. J Biol Chem. 286(42):36297-303 (2011)

  15. Wang, X. et al. Glia. 59(6):857-68 (2011)

  16. Mochizuki, N. Biol Pharm Bull. 34(2):260-5 (2011)

  17. Inamura, N. et al. Dev Neurosci. 33(2):118-29 (2011)

  18. Vidaki, M. et al. Cereb Cortex. (2011)

  19. Konno, A. et al. Neurosci Res. (2011)

  20. Gotoh, H. et al. Dev Biol. 349(2):504-11 (2011)

  21. Mains, RE. et al. BMC Neurosci. 12:20 (2011)

  22. Rubin, AN. et al. J Neurosci. 30(36):12050-62 (2010)

  23. Matsumoto, H. et al. J Neurophysiol. 103(6):3490-500 (2010)

  24. Tamura, H. et al. J Histochem Cytochem. 58(5):443-53 (2010)

  25. Asai, R. et al. Development. 137(22):3823-33 (2010)

  26. Hirota, Y. et al. Development. 137(18):3037-46 (2010)

  27. Honma, Y. et al. Development. 137(14):2319-28 (2010)

  28. Kohyama, J. J Cell Biol. 189(1):159-70 (2010)

  29. Manno, A. et al. Genes Cells. 15(8):911-22 (2010)

  30. Nakamura, M. et al. Molecular Vision 16, 425-437 (2010)

  31. Nishide, K. et al. PLoS ONE 4(8), e6869 (2009)

  32. Nagano, M. et al. The Journal of Cell Biology 183(7):1243-1257 (2008)

  33. Ono, K. et al. Development Biology 320(2):356-468 (2008)

  34. Nakashiba T. et al. Science 319(5867):1260-1264 (2008)

  35. Batista-Brito R. et al. The Journal of Neuroscience 28(15): 3966-3975 (2008)

  36. Esumi S. et al. Neuroscience Research 60(4):439-451 (2008)

  37. Fogarty M. et al. The Journal of Neuroscience 27(41):10935-10946 (2007)

  38. Sasamura T. et al. Development 134:1347-1356 (2007)

  39. Sato Y. et al. The Journal of Neuroscience 27(7):1606-1615 (2007)

  40. Yamada M. et al. Stem Cells 25(3):562-570 (2007)

  41. Togashi H. et al. The Journal of Cell Biology 174:141-151 (2006)

  42. Ogata M. et al. Molecular and Cell Biology 26(24):9220-9231 (2006)

  43. Sato Y. et al. The Journal of Neuroscience 25(20):4889-4897 (2005)

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Anti-GFP (Rat IgG2a), Monoclonal (GF090R) 04404-84 2-8 °C 200 µg 390.00 Buy
Anti-GFP (Rat IgG2a), Monoclonal (GF090R), Agarose Conjugate 06083-05 2-8 °C 500 µg 460.00 Buy

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