Anti-GFP (Rat IgG2a), Monoclonal (GF090R)
Features
- Immunohistochemical application
- Rat monoclonal antibody
Anti-GFP (Rat IgG2a), Monoclonal (GF090R)
| Clone | : | GF090R |
| Isotype | : | IgG2a (Rat) |
| Product form | : | Liquid |
| Immunogen | : | His-GFP (full length) fusion protein |
| Application | : | Immunohistochemistry 1:1000-1:2000 Western Blotting 1:1000-1:2000 ELISA 1:2000-1:20000 |
Anti-GFP (Rat IgG2a), Monoclonal (GF090R), Agarose Conjugate
| Antibody protein | : | ca. 2 mg antibody / ml agarose |
| Product form | : | 1:1 suspension in Dulbecco's PBS(-) |
Immunocytochemistry
| Sample | : | Mouse brain (nerve cell) |
| Primary antibody | : | Anti-GFP(Rat IgG2a), Monoclonal (1:1000), RT, O/N |
| Secondary antibody | : | Anti-Rat IgG-Cy3 (1:300), RT, 1hr |
| Blocking | : | 5% Normal goat serum/0.2% TritonX-100 in PBS |
| Fixing method | : | 4% Paraformaldehyde |
Data courtesy of Dr. Y. Yoshihara, RIKEN Brain Science Institute
Reference
- Yonehara, K. Nature. 469(7330):407-10 (2011)
- Abe, K. et al. Biol Reprod 85(5):1013-24 (2011)
- Hide, T. et al. Stem Cells. 29(4):590-9 (2011)
- Kurita, S. et al. J Biol Chem. 286(42):36297-303 (2011)
- Wang, X. et al. Glia. 59(6):857-68 (2011)
- Mochizuki, N. Biol Pharm Bull. 34(2):260-5 (2011)
- Inamura, N. et al. Dev Neurosci. 33(2):118-29 (2011)
- Vidaki, M. et al. Cereb Cortex. (2011)
- Konno, A. et al. Neurosci Res. (2011)
- Gotoh, H. et al. Dev Biol. 349(2):504-11 (2011)
- Mains, RE. et al. BMC Neurosci. 12:20 (2011)
- Rubin, AN. et al. J Neurosci. 30(36):12050-62 (2010)
- Matsumoto, H. et al. J Neurophysiol. 103(6):3490-500 (2010)
- Tamura, H. et al. J Histochem Cytochem. 58(5):443-53 (2010)
- Asai, R. et al. Development. 137(22):3823-33 (2010)
- Hirota, Y. et al. Development. 137(18):3037-46 (2010)
- Honma, Y. et al. Development. 137(14):2319-28 (2010)
- Kohyama, J. J Cell Biol. 189(1):159-70 (2010)
- Manno, A. et al. Genes Cells. 15(8):911-22 (2010)
- Nakamura, M. et al. Molecular Vision 16, 425-437 (2010)
- Nishide, K. et al. PLoS ONE 4(8), e6869 (2009)
- Nagano, M. et al. The Journal of Cell Biology 183(7):1243-1257 (2008)
- Ono, K. et al. Development Biology 320(2):356-468 (2008)
- Nakashiba T. et al. Science 319(5867):1260-1264 (2008)
- Batista-Brito R. et al. The Journal of Neuroscience 28(15): 3966-3975 (2008)
- Esumi S. et al. Neuroscience Research 60(4):439-451 (2008)
- Fogarty M. et al. The Journal of Neuroscience 27(41):10935-10946 (2007)
- Sasamura T. et al. Development 134:1347-1356 (2007)
- Sato Y. et al. The Journal of Neuroscience 27(7):1606-1615 (2007)
- Yamada M. et al. Stem Cells 25(3):562-570 (2007)
- Togashi H. et al. The Journal of Cell Biology 174:141-151 (2006)
- Ogata M. et al. Molecular and Cell Biology 26(24):9220-9231 (2006)
- Sato Y. et al. The Journal of Neuroscience 25(20):4889-4897 (2005)
Western Blotting
| Sample | : | 1. GFP, 2. GFP-14-3-3 (293 cell lysate 50 µg) |
| Gel | : | 5-20% Gradient gel |
| Primary antibody | : | Anti-GFP(Rat IgG2a), Monoclonal (3 µg/ml), O/N |
| Secondary antibody | : | Anti-Rat IgG-HRP (1:10000) |
Data courtesy of Dr. K. Yoshida, Depertment of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University.
Immunoprecipitation : Anti-GFP (Rat IgG2a), Agarose Conjugate
Protocol
- Add Anti-GFP (Rat IgG2a), agarose conjugate (20 µl) in 293 Cell Lysate (1 ml) expressed EGFP or EGFP-myosin (1ml each). Incubate at 4°C for 1 hour.
- Add SDS Sample buffer. After heating the samples at 90°C for 10 minutes, load to SDS-PAGE.
- Detect EGFP and EGFP-myosin using Anti-GFP (Rat IgG2a).
Data courtesy of Prof. A. Kakizuka, Laboratory of Functional Biology, Kyoto University Graduate School of Biostudies.
Reference
- Kumeta, M. et al. J Cell Sci. 123(Pt 7):1020-30 (2010)