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Home > Products > Life Sciences > Protein Research > Western Blotting > Anti-GFP (Rat IgG2a), Monoclonal (GF090R)

Anti-GFP (Rat IgG2a), Monoclonal (GF090R)

Features

  • Immunohistochemical application
  • Rat monoclonal antibody

Anti-GFP (Rat IgG2a), Monoclonal (GF090R)

Clone : GF090R
Isotype : IgG2a (Rat)
Product form : Liquid
Immunogen : His-GFP (full length) fusion protein
Application : Immunohistochemistry 1:1000-1:2000
Western Blotting 1:1000-1:2000
ELISA 1:2000-1:20000

Anti-GFP (Rat IgG2a), Monoclonal (GF090R), Agarose Conjugate

Antibody protein : ca. 2 mg antibody / ml agarose
Product form : 1:1 suspension in Dulbecco's PBS(-)

Western Blotting

Sample : 1. GFP, 2. GFP-14-3-3 (293 cell lysate 50 µg)
Gel : 5-20% Gradient gel
Primary antibody : Anti-GFP(Rat IgG2a), Monoclonal (3 µg/ml), O/N
Secondary antibody : Anti-Rat IgG-HRP (1:10000)

Data courtesy of Dr. K. Yoshida, Depertment of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University.

Immunoprecipitation : Anti-GFP (Rat IgG2a), Agarose Conjugate

Protocol

  1. Add Anti-GFP (Rat IgG2a), agarose conjugate (20 µl) in 293 Cell Lysate (1 ml) expressed EGFP or EGFP-myosin (1ml each). Incubate at 4°C for 1 hour.
  2. Add SDS Sample buffer. After heating the samples at 90°C for 10 minutes, load to SDS-PAGE.
  3. Detect EGFP and EGFP-myosin using Anti-GFP (Rat IgG2a).

Data courtesy of Prof. A. Kakizuka, Laboratory of Functional Biology, Kyoto University Graduate School of Biostudies.

Reference
  1. Kumeta, M. et al. J Cell Sci. 123(Pt 7):1020-30 (2010)

Immunocytochemistry

Sample : Mouse brain (nerve cell)
Primary antibody : Anti-GFP(Rat IgG2a), Monoclonal (1:1000), RT, O/N
Secondary antibody : Anti-Rat IgG-Cy3 (1:300), RT, 1hr
Blocking : 5% Normal goat serum/0.2% TritonX-100 in PBS
Fixing method : 4% Paraformaldehyde

Data courtesy of Dr. Y. Yoshihara, RIKEN Brain Science Institute

Reference

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  4. Kim, Hyunsoo, et al. "Wiring patterns from auditory sensory neurons to the escape and song‐relay pathways in fruit flies." Journal of Comparative Neurology (2020).
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Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Anti-GFP (Rat IgG2a), Monoclonal (GF090R) 04404-84 2-8 °C 200 µg 390.00 Buy
Anti-GFP (Rat IgG2a), Monoclonal (GF090R), Agarose Conjugate 06083-05 2-8 °C 500 µg 460.00 Buy