| Product Name | Chemi-Lumi One L | Chemi-Lumi One Super | Chemi-Lumi One Ultra |
|---|---|---|---|
| Product No. | 07880-70 | 02230-30 | 11644-40 |
| Usage | General Use | High Sensitive | Ultra high Sensitive |
| Lower Detection Limit | Low-picogram | Mid-femtogram | Low-femtogram |
| Required Working Solution | approx. 0.125 ml / cm2 | approx. 0.1 ml / cm2 | approx. 0.1 ml / cm2 |
| Suggested Antibody Dilution | Primary: 1:1,000-1:5,000 Secondary: 1:20,000-1:100,000 |
Primary: 1:1,000-1:20,000 Secondary: 1:20,000-1:200,000 |
Primary: 1:5,000-1:100,000 Secondary: 1:100,000-1:500,000 |
| Reaction Period | 1 min. | 1 min. | 5 min. |
| Comparable to | Amersham TM ECLTM SuperSignal® West Pico® |
Amersham TM ECLTM Prime SuperSignal® West Dura® |
Amersham TM ECLTM Select SuperSignal® West Femto® |
| Sensitivity |
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Chemiluminescent HRP Substrates : Chemi-Lumi One Super
Mid-femtogram Sensitivity Substrate for HRP / Economical - costs much less than GE ECL Prime Substrate
Chemi-Lumi One is a series of high sensitive luminol-based chemiluminescence assay kits for Western Blotting. We offer three types of chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP).
Features
- Mid-femtogram sensitivity - much more sensitive than Amersham TM ECLTM Prime Substrate
- Economical - costs much less than Amersham TM ECLTM Prime Substrate
Chemi-Lumi One Series for HRP
Chemi-Lumi One is a series of high sensitive luminol-based chemiluminescence assay kits for Western Blotting. We offer three types of chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP).
Comparison of sensitivity
Chemi-Lumi One Super is much more sensitive than GE ECL Prime Substrate. Western blot of identical biotinylated myosin samples probed with respective streptavidin-HRP conjugates. The membranes were incubated with substrate that was prepared according to the manufacturers' instructions. The membranes were exposed to film for 2 minutes
Reference
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Watanabe, Miyuki, Zakaria Omahdi, and Sho Yamasaki. "Direct Binding Analysis Between C-Type Lectins and Glycans Using Immunoglobulin Receptor Fusion Proteins." Lectin Purification and Analysis. Humana, New York, NY, 2020. 119-128.
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Hagiwara, Asami, et al. "Induction of tryptophan hydroxylase in the liver of sc tumor model of prostate cancer." Cancer Science 111.4 (2020): 1218.
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Sakai, Maiko, et al. "Isorhamnetin, a 3’-methoxylated flavonol, enhances the lysosomal proteolysis in J774. 1 murine macrophages in a TFEB-independent manner." Bioscience, Biotechnology, and Biochemistry (2020): 1-11.
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Sanada, Taiki, et al. "Anti-inflammatory effects of miRNA-146a induced in adipose and periodontal tissues." Biochemistry and Biophysics Reports 22 (2020): 100757.
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Masaki, So, et al. "The cysteine residue at 424th of pyruvate kinase M2 is crucial for tetramerization and responsiveness to oxidative stress." Biochemical and Biophysical Research Communications (2020).
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Larasati, Tamara, et al. "Tmprss12 is required for sperm motility and uterotubal junction migration in mice." Biology of Reproduction (2020).
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Kunimoto, Hiroyuki, et al. "RBM10 regulates centriole duplication in HepG2 cells by ectopically assembling PLK4‐STIL complexes in the nucleus." Genes to Cells 25.2 (2020): 100-110.
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Tozaki-Saitoh, Hidetoshi, et al. "Involvement of exchange protein directly activated by cAMP and tumor progression locus 2 in IL-1β production in microglial cells following activation of β-adrenergic receptors." Journal of Pharmacological Sciences (2020).
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Kawamura, Aki, et al. "Combined intake of astaxanthin, β-carotene, and resveratrol elevates protein synthesis during muscle hypertrophy in mice." Nutrition 69 (2020): 110561.
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Yotsumoto, Karen, et al. "Amelogenin Downregulates Interferon Gamma-Induced Major Histocompatibility Complex Class II Expression Through Suppression of Euchromatin Formation in the Class II Transactivator Promoter IV Region in Macrophages." Frontiers in Immunology 11 (2020): 709.
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Tone, Takuya, et al. "ATPase reaction cycle of P4‐ATPases affects their transport from the endoplasmic reticulum." FEBS letters 594.3 (2020): 412-423.
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Tanaka, Kentaro, et al. "Ubiquitination of Src promotes its secretion via small extracellular vesicles." Biochemical and Biophysical Research Communications 525.1 (2020): 184-191.
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Kobayashi, Kiyonori, et al. "Prss55 but not Prss51 is required for male fertility in mice." Biology of Reproduction (2020).
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Fujinami, Hiroyuki, et al. "CLP1 acts as the main RNA kinase in mice." Biochemical and Biophysical Research Communications 525.1 (2020): 129-134.
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Tsuji, Gaku, et al. "Aryl Hydrocarbon Receptor Activation Downregulates IL-33 Expression in Keratinocytes via Ovo-Like 1." Journal of clinical medicine 9.3 (2020): 891.
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Khafagy, El-Sayed, et al. "Systemic and brain delivery of leptin via intranasal coadministration with cell-penetrating peptides and its therapeutic potential for obesity." Journal of Controlled Release (2020).
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Nozato, Yoichi, et al. "Novel properties of myoferlin in glucose metabolism via pathways involving modulation of adipose functions." The FASEB Journal (2020).
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Nakamura, Yuto, et al. "A disintegrin and metalloproteinase 12 prevents heart failure by regulating cardiac hypertrophy and fibrosis." American Journal of Physiology-Heart and Circulatory Physiology 318.2 (2020): H238-H251.
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Chemi Lumi One Super (Instruction)Comparison of price
|
Company
|
Nacalai USA, Inc.
|
Cytiva
|
|---|---|---|
|
Item
|
Chemi-Lumi One Super
|
Amersham ECL Prime
Western Blotting Detection Reagent
|
|
Price
|
$210
|
$345
|
|
Cat. No.
|
02230-30
|
RPN2232
|
|
Quantity
|
for 1,000 cm² membrane
|
for 1,000 cm² membrane
|
Ordering Information
| Product | Storage | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| Chemi-Lumi One Super: Luminol 10 mL, Peroxide 10 mL (Sufficient substrate for 200 cm² of blotting membrane) |
4°C | 02230-14 | 2 x 10 mL | 92.00 | Buy |
| Chemi-Lumi One Super: Luminol 50 mL, Peroxide 50 mL (Sufficient substrate for 1,000 cm² of blotting membrane) |
4°C | 02230-30 | 1 kit | 231.00 | Buy |