Chemi-Lumi One is a series of high sensitive luminol-based chemiluminescence assay kits for Western Blotting. We offer three types of chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP).
<Condition> Antigen: Anti-Mouse IgG (Goat), HRP Conjugated (Santa Cruz, sc-2005) Detection: L (1 min. ), Super (1 min. ) and Ultra (5 min. ) Detector: LAS-3000 Super mode (Analyze 3 min. later after reaction with each substrate) Expose time: 30 min.
Comparison of sensitivity and duration
Chemi-Lumi One L has more sensitive and longer signal duration than GE ECL Substrate. Western blot of identical biotinylated samples probed with respective streptavidin-HRP conjugates. The membranes were incubated with substrate that was prepared according to the manufacturers' instructions.
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Chemi-Lumi One L (Luminol 250 mL, Peroxide 250 mL,
Sufficient substrate for 4,000 cm² of blotting membrane)