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Chemiluminescent HRP Substrates : Chemi-Lumi One L

Picogram Sensitivity Substrate for HRP / Economical - costs much less than other ECL substrate


  • Picogram sensitivity - better signal than Amersham TM ECLTM Substrate
  • Economical - costs much less than other Amersham TM ECLTM Substrate
  • Long light emission


Chemi-Lumi One Series for HRP

Chemi-Lumi One is a series of high sensitive luminol-based chemiluminescence assay kits for Western Blotting. We offer three types of chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP).

Product NameChemi-Lumi One LChemi-Lumi One SuperChemi-Lumi One Ultra
Product No. 07880-70 02230-30 11644-40
Usage General Use High Sensitive Ultra high Sensitive
Lower Detection Limit Low-picogram Mid-femtogram Low-femtogram
Required Working Solution approx. 0.125 ml / cm2 approx. 0.1 ml / cm2 approx. 0.1 ml / cm2
Suggested Antibody Dilution Primary: 1:1,000-1:5,000
Secondary: 1:20,000-1:100,000
Primary: 1:1,000-1:20,000
Secondary: 1:20,000-1:200,000
Primary: 1:5,000-1:100,000
Secondary: 1:100,000-1:500,000
Reaction Period 1 min. 1 min. 5 min.
Comparable to Amersham TM ECLTM
SuperSignal® West Pico®
Amersham TM ECLTM Prime
SuperSignal® West Dura®
Amersham TM ECLTM Select
SuperSignal® West Femto®


Antigen: Anti-Mouse IgG (Goat), HRP Conjugated (Santa Cruz, sc-2005)
Detection: L (1 min. ), Super (1 min. ) and Ultra (5 min. )
Detector: LAS-3000 Super mode (Analyze 3 min. later after reaction with each substrate)
Expose time: 30 min.

Comparison of sensitivity and duration

Chemi-Lumi One L has more sensitive and longer signal duration than GE ECL Substrate. Western blot of identical biotinylated samples probed with respective streptavidin-HRP conjugates. The membranes were incubated with substrate that was prepared according to the manufacturers' instructions.
Lane1: 1: 50 dilution Lane3: 1: 200
Lane2: 1: 100 dilution Lane4: 1: 400


  1. Yamashita, Kaoru, et al. "Adrenal cortex hypoxia modulates aldosterone production in heart failure." Biochemical and Biophysical Research Communications (2020).

  2. Chihara, Yusuke, et al. "Histone deacetylase inhibitor OBP‑801 and amrubicin synergistically inhibit the growth of squamous cell lung carcinoma by inducing mitochondrial ASK1‑dependent apoptosis." International Journal of Oncology 56.3 (2020): 848-856.

  3. Sheikh, Amin Alborzian Deh, Chizuru Akatsu, and Takeshi Tsubata. "Identification of Siglec Cis-Ligands by Proximity Labeling." Lectin Purification and Analysis. Humana, New York, NY, 2020. 75-83.

  4. Roudkenar, Mehryar Habibi, et al. "Disturbance in the regulation of miR 17-92 cluster on HIF-1-α expression contributes to clinically relevant radioresistant cells: an in vitro study." Cytotechnology (2020): 1-13.

  5. Umekawa, Midori, et al. "Mitotic cyclin Clb4 is required for the intracellular adaptation to glucose starvation in Saccharomyces cerevisiae." FEBS letters 594.8 (2020): 1329-1338.

  6. Norikura, Toshio, et al. "cis-Banglene, a bangle (Zingiber purpureum)-derived bioactive compound, promotes mitochondrial biogenesis and glucose uptake by activating the IL-6/AMPK signaling pathway in C2C12 skeletal muscle cells." Journal of Functional Foods 64 (2020): 103632.

  7. Kasahara, Takaoki, et al. "Cardiolipin is essential for early embryonic viability and mitochondrial integrity of neurons in mammals." The FASEB Journal (2020).

  8. Uehara, Natsumi, et al. "Oncolytic effect of Midkine promoter–based conditionally replicating adenoviruses expressing EGFR siRNA in head and neck squamous cancer cell line T891." Cancer Reports.

Comparison of price

Nacalai USA, Inc.
Chemi-Lumi One L
Amersham ECL
Western Blotting Detection Reagent
Cat. No.
for 4,000 cm² membrane
for 4,000 cm² membrane

Ordering Information

Product Storage Cat.No. PKG Size Price  
Chemi-Lumi One L (Luminol 250 mL, Peroxide 250 mL,
Sufficient substrate for 4,000 cm² of blotting membrane)
4°C 07880-70 1 kit 200.00 Buy
Chemi-Lumi One L (Luminol 50 mL, Peroxide 50 mL,
Sufficient substrate for 800 cm² of blotting membrane)
4°C 07880-54 2 x 50 ml 84.00 Buy