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Chemiluminescent HRP Substrates : Chemi-Lumi One Super

Mid-femtogram Sensitivity Substrate for HRP / Economical - costs much less than GE ECL Prime Substrate


  • Mid-femtogram sensitivity - much more sensitive than Amersham TM ECLTM Prime Substrate
  • Economical - costs much less than Amersham TM ECLTM Prime Substrate


Chemi-Lumi One Series for HRP

Chemi-Lumi One is a series of high sensitive luminol-based chemiluminescence assay kits for Western Blotting. We offer three types of chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP).

Product NameChemi-Lumi One LChemi-Lumi One SuperChemi-Lumi One Ultra
Product No. 07880-70 02230-30 11644-40
Usage General Use High Sensitive Ultra high Sensitive
Lower Detection Limit Low-picogram Mid-femtogram Low-femtogram
Required Working Solution approx. 0.125 ml / cm2 approx. 0.1 ml / cm2 approx. 0.1 ml / cm2
Suggested Antibody Dilution Primary: 1:1,000-1:5,000
Secondary: 1:20,000-1:100,000
Primary: 1:1,000-1:20,000
Secondary: 1:20,000-1:200,000
Primary: 1:5,000-1:100,000
Secondary: 1:100,000-1:500,000
Reaction Period 1 min. 1 min. 5 min.
Comparable to Amersham TM ECLTM
SuperSignal® West Pico®
Amersham TM ECLTM Prime
SuperSignal® West Dura®
Amersham TM ECLTM Select
SuperSignal® West Femto®


Antigen: Anti-Mouse IgG (Goat), HRP Conjugated (Santa Cruz, sc-2005)
Detection: L (1 min. ), Super (1 min. ) and Ultra (5 min. )
Detector: LAS-3000 Super mode (Analyze 3 min. later after reaction with each substrate)
Expose time: 30 min.

Comparison of sensitivity

Chemi-Lumi One Super is much more sensitive than GE ECL Prime Substrate. Western blot of identical biotinylated myosin samples probed with respective streptavidin-HRP conjugates. The membranes were incubated with substrate that was prepared according to the manufacturers' instructions. The membranes were exposed to film for 2 minutes


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Comparison of price

Nacalai USA, Inc.
Chemi-Lumi One Super
Amersham ECL Prime
Western Blotting Detection Reagent
Cat. No.
for 1,000 cm² membrane
for 1,000 cm² membrane

Ordering Information

Product Storage Cat.No. PKG Size Price  
Chemi-Lumi One Super (Luminol 50 mL, Peroxide 50 mL, Sufficient substrate for 1,000 cm² of blotting membrane) 4°C 02230-30 1 kit 210.00 Buy
Chemi-Lumi One Super (Luminol 10 mL, Peroxide 10 mL, Sufficient substrate for 200 cm² of blotting membrane) 4°C 02230-14 2 x 10 mL 84.00 Buy