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Chemiluminescent HRP Substrates : Chemi-Lumi One Super

Mid-femtogram Sensitivity Substrate for HRP / Economical - costs much less than GE ECL Prime Substrate

Features

  • Mid-femtogram sensitivity - much more sensitive than Amersham TM ECLTM Prime Substrate
  • Economical - costs much less than Amersham TM ECLTM Prime Substrate

 

Chemi-Lumi One Series for HRP

Chemi-Lumi One is a series of high sensitive luminol-based chemiluminescence assay kits for Western Blotting. We offer three types of chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP).

Product NameChemi-Lumi One LChemi-Lumi One SuperChemi-Lumi One Ultra
Product No. 07880-70 02230-30 11644-40
Usage General Use High Sensitive Ultra high Sensitive
Lower Detection Limit Low-picogram Mid-femtogram Low-femtogram
Required Working Solution approx. 0.125 ml / cm2 approx. 0.1 ml / cm2 approx. 0.1 ml / cm2
Suggested Antibody Dilution Primary: 1:1,000-1:5,000
Secondary: 1:20,000-1:100,000
Primary: 1:1,000-1:20,000
Secondary: 1:20,000-1:200,000
Primary: 1:5,000-1:100,000
Secondary: 1:100,000-1:500,000
Reaction Period 1 min. 1 min. 5 min.
Comparable to Amersham TM ECLTM
SuperSignal® West Pico®
Amersham TM ECLTM Prime
SuperSignal® West Dura®
Amersham TM ECLTM Select
SuperSignal® West Femto®
Sensitivity

  

<Condition>
Antigen: Anti-Mouse IgG (Goat), HRP Conjugated (Santa Cruz, sc-2005)
Detection: L (1 min. ), Super (1 min. ) and Ultra (5 min. )
Detector: LAS-3000 Super mode (Analyze 3 min. later after reaction with each substrate)
Expose time: 30 min.

Comparison of sensitivity

Chemi-Lumi One Super is much more sensitive than GE ECL Prime Substrate. Western blot of identical biotinylated myosin samples probed with respective streptavidin-HRP conjugates. The membranes were incubated with substrate that was prepared according to the manufacturers' instructions. The membranes were exposed to film for 2 minutes

Comparison of price

Company
Nacalai USA, Inc.
Cytiva
Item
Chemi-Lumi One Super
Amersham ECL Prime
Western Blotting Detection Reagent
Price
$200
$265
Cat. No.
02230-30
RPN2232
Quantity
for 1,000 cm² membrane
for 1,000 cm² membrane

Reference

  1. Watanabe, Miyuki, Zakaria Omahdi, and Sho Yamasaki. "Direct Binding Analysis Between C-Type Lectins and Glycans Using Immunoglobulin Receptor Fusion Proteins." Lectin Purification and Analysis. Humana, New York, NY, 2020. 119-128.

  2. Hagiwara, Asami, et al. "Induction of tryptophan hydroxylase in the liver of sc tumor model of prostate cancer." Cancer Science 111.4 (2020): 1218.

  3. Sakai, Maiko, et al. "Isorhamnetin, a 3’-methoxylated flavonol, enhances the lysosomal proteolysis in J774. 1 murine macrophages in a TFEB-independent manner." Bioscience, Biotechnology, and Biochemistry (2020): 1-11.

  4. Sanada, Taiki, et al. "Anti-inflammatory effects of miRNA-146a induced in adipose and periodontal tissues." Biochemistry and Biophysics Reports 22 (2020): 100757.

  5. Masaki, So, et al. "The cysteine residue at 424th of pyruvate kinase M2 is crucial for tetramerization and responsiveness to oxidative stress." Biochemical and Biophysical Research Communications (2020).

  6. Larasati, Tamara, et al. "Tmprss12 is required for sperm motility and uterotubal junction migration in mice." Biology of Reproduction (2020).

  7. Kunimoto, Hiroyuki, et al. "RBM10 regulates centriole duplication in HepG2 cells by ectopically assembling PLK4‐STIL complexes in the nucleus." Genes to Cells 25.2 (2020): 100-110.

  8. Tozaki-Saitoh, Hidetoshi, et al. "Involvement of exchange protein directly activated by cAMP and tumor progression locus 2 in IL-1β production in microglial cells following activation of β-adrenergic receptors." Journal of Pharmacological Sciences (2020).

  9. Kawamura, Aki, et al. "Combined intake of astaxanthin, β-carotene, and resveratrol elevates protein synthesis during muscle hypertrophy in mice." Nutrition 69 (2020): 110561.

  10. Yotsumoto, Karen, et al. "Amelogenin Downregulates Interferon Gamma-Induced Major Histocompatibility Complex Class II Expression Through Suppression of Euchromatin Formation in the Class II Transactivator Promoter IV Region in Macrophages." Frontiers in Immunology 11 (2020): 709.

  11. Tone, Takuya, et al. "ATPase reaction cycle of P4‐ATPases affects their transport from the endoplasmic reticulum." FEBS letters 594.3 (2020): 412-423.

  12. Tanaka, Kentaro, et al. "Ubiquitination of Src promotes its secretion via small extracellular vesicles." Biochemical and Biophysical Research Communications 525.1 (2020): 184-191.

  13. Kobayashi, Kiyonori, et al. "Prss55 but not Prss51 is required for male fertility in mice." Biology of Reproduction (2020).

  14. Fujinami, Hiroyuki, et al. "CLP1 acts as the main RNA kinase in mice." Biochemical and Biophysical Research Communications 525.1 (2020): 129-134.

  15. Tsuji, Gaku, et al. "Aryl Hydrocarbon Receptor Activation Downregulates IL-33 Expression in Keratinocytes via Ovo-Like 1." Journal of clinical medicine 9.3 (2020): 891.

  16. Khafagy, El-Sayed, et al. "Systemic and brain delivery of leptin via intranasal coadministration with cell-penetrating peptides and its therapeutic potential for obesity." Journal of Controlled Release (2020).

  17. Nozato, Yoichi, et al. "Novel properties of myoferlin in glucose metabolism via pathways involving modulation of adipose functions." The FASEB Journal (2020).

  18. Nakamura, Yuto, et al. "A disintegrin and metalloproteinase 12 prevents heart failure by regulating cardiac hypertrophy and fibrosis." American Journal of Physiology-Heart and Circulatory Physiology 318.2 (2020): H238-H251.

  19. Shibagaki, Fumiya, et al. "Systemic Administration of an Apelin Receptor Agonist Prevents NMDA-Induced Loss of Retinal Neuronal Cells in Mice." Neurochemical Research (2020): 1-8.

  20. Uemura, Norihito, et al. "Limited spread of pathology within the brainstem of α-synuclein BAC transgenic mice inoculated with preformed fibrils into the gastrointestinal tract." Neuroscience Letters 716 (2020): 134651.

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Chemi-Lumi One Super (Luminol 50 mL, Peroxide 50 mL, Sufficient substrate for 1,000 cm² of blotting membrane) 02230-30 4°C 1 kit 200.00 Buy
Chemi-Lumi One Super (Luminol 10 mL, Peroxide 10 mL, Sufficient substrate for 200 cm² of blotting membrane) 02230-14 4°C 2 x 10 mL 80.00 Buy