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Chemiluminescent HRP Substrates : Chemi-Lumi One Ultra

Low-femtogram Sensitivity Substrate for HRP / The Highest Sensitivity


  • Low-femtogram sensitivity 
  • Longer signal duration


Chemi-Lumi One Series for HRP

Chemi-Lumi One is a series of high sensitive luminol-based chemiluminescence assay kits for Western Blotting. We offer three types of chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP).

Product NameChemi-Lumi One LChemi-Lumi One SuperChemi-Lumi One Ultra
Product No. 07880-70 02230-30 11644-40
Usage General Use High Sensitive Ultra high Sensitive
Lower Detection Limit Low-picogram Mid-femtogram Low-femtogram
Required Working Solution approx. 0.125 ml / cm2 approx. 0.1 ml / cm2 approx. 0.1 ml / cm2
Suggested Antibody Dilution Primary: 1:1,000-1:5,000
Secondary: 1:20,000-1:100,000
Primary: 1:1,000-1:20,000
Secondary: 1:20,000-1:200,000
Primary: 1:5,000-1:100,000
Secondary: 1:100,000-1:500,000
Reaction Period 1 min. 1 min. 5 min.
Comparable to Amersham TM ECLTM
SuperSignal® West Pico®
Amersham TM ECLTM Prime
SuperSignal® West Dura®
Amersham TM ECLTM Select
SuperSignal® West Femto®


Antigen: Anti-Mouse IgG (Goat), HRP Conjugated (Santa Cruz, sc-2005)
Detection: L (1 min. ), Super (1 min. ) and Ultra (5 min. )
Detector: LAS-3000 Super mode (Analyze 3 min. later after reaction with each substrate)
Expose time: 30 min.

Comparison of Sensitivity with SuperSigmal West Femto

Chemi-Lumi One Ultra is an extremely sensitive chemiluminescent substrate. Western blot of identical transferrin samples. The membranes were incubated with substrate that was prepared according to the manufacturers' instructions. The membranes were exposed to film for 2 minutes.                                   
  - Primary antibody : Rabbit anti-transferrin, 1: 30,000
  - Secondary antibody: Goat anti-rabbit IgG-HRP, 1: 200,000

Comparison of Sensitivity with Competitors

Chemi-Lumi One Ultra shows equal to or higher sensitivity compared to competitors'

Gel: 10% PAGE gel
Wash: 0.1% t-TBS (x1), pH 7.4
Blocking: Blocking One (Cat. No.: 03953)
1st Ab: Anti-β-Actin Mouse Monoclonal Antibody (Santa Cruz, Cat. No.: sc-47778), 1:300
2nd Ab: Anti-Mouse IgG (Goat), HRP Conjugated (Santa Crus, Cat. No.: sc-2005), 1: 1000
Detection Period: 5 min.
Detector: LAS-3000 High Mode
Expose Time: 3 min.

Comparison of Abs Concentration

Due to low background, Chemi-Lumi One Ultra is applicable to wide range of primary antibodies, and makes it easy to optimize condition.

Gel: 10% PAGE gel
Wash: 0.1% t-TBS (x1), pH 7.4
Blocking: Blocking One (Cat. No.: 03953)
1st Ab: Anti-PCNA Mouse Monoclonal Antibody (Santa Cruz, Cat. No.: sc-56c)
2nd Ab: Anti-Mouse IgG (Goat), HRP Conjugated (Santa Crus, Cat. No.: sc-2005), 1: 100,000
Detection Period: 5 min.
Detector: LAS-3000 High Mode
Expose Time: 1:1000, 3 min. / 1:5000, 5 min. / 1:10,000, 8 min.


  1. Iwasaki, Takeshi, et al. "Establishment and Characterization of a Novel Primitive Yolk Sac Tumour Cell Line, TC587." Anticancer research 40.2 (2020): 759-766.

  2. Saito, Akatsuki, et al. "The 4th and 112th residues of viral capsid cooperatively modulate capsid-CPSF6 interactions of HIV-1." AIDS Research and Human Retroviruses ja (2020).

  3. Kitakaze, Tomoya, et al. "Extracellular transglutaminase 2 induces myotube hypertrophy through G protein-coupled receptor 56." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1867.2 (2020): 118563.

  4. Dang-Nguyen, Thanh Quang, et al. "Combined refinements to somatic cell nuclear transfer methods improve porcine embryo development." Journal of Reproduction and Development (2020): 2019-156.

  5. Haraguchi, Seiki, et al. "Establishment of porcine nuclear transfer-derived embryonic stem cells using induced pluripotent stem cells as donor nuclei." Journal of Reproduction and Development (2020): 2019-137.

  6. Chen, Chen, et al. "Isolation of a novel bacterial strain capable of producing abundant extracellular membrane vesicles carrying a single major cargo protein and analysis of its transport mechanism." Frontiers in Microbiology 10 (2020): 3001.

  7. Noguchi, Taiki, et al. "Regulatory expression of bone morphogenetic protein 6 by 2, 2′-dipyridyl." Biochimica et Biophysica Acta (BBA)-General Subjects (2020): 129610.

Comparison of Price

Nacalai USA, Inc.
Chemi-Lumi One Ultra
Amersham ECL Select
Western Blotting Detection Reagent
Cat. No.
for 1,000 cm² membrane
for 1,000 cm² membrane

Ordering Information

Product Storage Cat.No. PKG Size Price  
Chemi-Lumi One Ultra (Luminol 50 mL, Peroxide 50 mL, Sufficient substrate for 1,000 cm² of blotting membrane) Room Temp. 11644-40 1 Kit 328.00 Buy
Chemi-Lumi One Ultra (Luminol 10 mL, Peroxide 10 mL, Sufficient substrate for 200 cm² of blotting membrane) Room Temp. 11644-24 1 Kit 116.00 Buy