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EZSPHERE™

Cell Culture Ware for Spheroid Formation


Three dimensional (3D) cell culture systems have gained in popularity as invaluable tools in broad applications of cell biology. 3D multi-cellular cell aggregates (Spheroid) can be formed by using a low attachment culture surface. However, variability in forming spheroids has been a persistent problem. EZSPHERE is specifically designed to form a large number of uniformly sized Spheroids and Embryoid Bodies (EBs).

Features

  • The surface of EZSPHERE is coated with very low binding 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer.
  • EZSPHERE has a lot of evenly designed micro wells on the surface -- 6 different well size available. 
    Micro well type

     Diameter (µm) 

        Depth (µm)    

     

    #901 200 100
    #900 500 100
    #902 500 200
    #903 800 400
    #904 800 300
    #905 1,400 600

Publication

"Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells"

http://www.nature.com/articles/srep31063

Unique micro-fabricated plastic vessel

Useful for mass-production of EBs with uniform size

EB formation, proliferation and differentiation protocols

High efficient generation of EBs with uniform size

EB-size control with micro-well sizes or inoculating cell densities

Proliferation of iPSCs with maintaining pluripotency

Induction of dopaminergic neurons from the EBs

Induction of cardiomyocyte from the EBs

Referred protocol / References

Referred protocol: 

iPS spheroid formation in EZSPHERETM devices: http://www.atgc.co.jp/div/rika/hbin/ez2012.html

 

 

References: 

1. Zhang RR, Takebe T, Miyazaki L, Takayama M, Koike H, Kimura M, Enomura M, Zheng YW, Sekine K, Taniguchi H
Efficient Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Three-Dimensional Microscale Culture
Stem Cells and Tissue Repair Methods in Molecular Biology Volume 1210, 2014, pp 131-141
 
2. Shimada H, Hashimoto Y, Nakada A, Shigeno K, Nakamura T,
Accelerated generation of human induced pluripotent stem cells with retroviral transduction and chemical inhibitors under physiological hypoxia
Biochemical and Biophysical Research Communications, Volume 417, Issue 2, Pages 659-664
 
3. Aikawa N, Suzuki Y, Takaba K
A Simple Protocol for the Myocardial Differentiation of Human iPS Cells
Biological and Pharmaceutical Bulletin Vol. 38 (2015) No. 7 Pages 1070-1075
 
4. Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T
TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues
Scientific Reports 6, Article number: 21747 (2016) doi:10.1038/srep21747
 
5. Hiroki Sato, Alimjan Idiris, Tatsuaki Miwa, Hiromichi Kumagai
Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
Scientific Reports 6, Article number: 31063 (2016) doi:10.1038/srep31063
 
6. Taku Nakayama, Shimpei Otsuka, Tatsuya Kobayashi, Hodaka Okajima, Kentaro Matsumoto, Yuichiro Hagiya, Keiji Inoue Taro Shuin, Motowo Nakajima, Tohru Tanaka, Shun-ichiro Ogura
Dormant cancer cells accumulate high protoporphyrin IX levels and are sensitive to 5-aminolevulinic acid-based photodynamic therapy
Scientific Reports 6, Article number: 36478 (2016)doi:10.1038/srep36478
 
7. Ayako Aihara, Natsuki Abe, Koichiro Saruhashi, Tatsuro Kanaki, Taito Nishino
Novel 3-D cell culture system for in vitro evaluation of anticancer drugs under anchorage-independent conditions
Cancer ScienceVolume 107, Issue 12 December 2016 Pages 1858−1866 DOI: 10.1111/cas.13095
 
8. Waseem K. Raja, Alison E. Mungenast, Yuan-Ta Lin, Tak Ko, Fatema Abdurrob, Jinsoo Seo, Li-Huei Tsai
Self-Organizing 3D Human Neural Tissue Derived from Induced Pluripotent Stem Cells Recapitulate AlzheimerÕs Disease Phenotypes
PLOS ONE 11(9): e0161969. doi: 10.1371/journal.pone.0161969
 
9. Hiroyuki Koike, Ran-Ran Zhang, Yasuharu Ueno, Keisuke Sekine, Yun-Wen Zheng, Takanori Takebe, Hideki Taniguchi
Development 2017 144: 1018-1024; doi: 10.1242/dev.143032
 
10. Sugimura, R., Jha, D. K., Han, A., Soria-Valles, C., Da Rocha, E. L., Lu, Y. F., ... & Wong, I. (2017).
Nature545(7655), 432-438.

 

Ordering Information

#900
Product Cat.No. Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish [Diameter: 500µm, Depth: 100µm, No. of Well: 2,700/dish] 4000-900SP Room Temp. 10 dishes 430.00 Buy
EZSPHERE™ 60 mm Dish [Diameter: 500µm, Depth: 100µm, No. of Well: 6,500/dish] 4010-900SP Room Temp. 10 dishes 460.00 Buy
EZSPHERE™ 100 mm Dish [Diameter: 500µm, Depth: 100µm, No. of Well: 17,000/dish] 4020-900SP Room Temp. 10 dishes 490.00 Buy
EZSPHERE™ 6-well Plate [Diameter: 500µm, Depth: 100µm, No. of Well: 2,700/well] 4810-900SP Room Temp. 5 plates 265.00 Buy
EZSPHERE™ 24-well Plate [Diameter: 500µm, Depth: 100µm, No. of Well: 470/well] 4820-900SP Room Temp. 5 plates 265.00 Buy
EZSPHERE™ 96-well Plate [Diameter: 500µm, Depth: 100µm, No. of Well: 95/well] 4860-900SP Room Temp. 5 plates 265.00 Buy
#901
Product Cat.No. Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish [Diameter: 200µm, Depth: 100µm] TCI-4000-901SP Room Temp. 10 dishes 690.00 Buy
#902
Product Cat.No. Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish [Diameter: 500µm, Depth: 200µm, No. of Well: 2,700/dish] 4000-902SP Room Temp. 10 dishes 440.00 Buy
#903
Product Cat.No. Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish [Diameter: 800µm, Depth: 400µm, No. of Well: 1,250/dish] 4000-903SP Room Temp. 10 dishes 440.00 Buy
EZSPHERE™ 12-well Plate [Diameter: 800µm, Depth: 400µm] TCI-4815-903SP-10P Room Temp. 10 plates 930.00 Buy
EZSPHERE™ 12-well Plate [Diameter: 800µm, Depth: 400µm] TCI-4815-903SP-50P Room Temp. 50 plates 3,150.00 Buy
EZSPHERE™ 96-well Plate [Diameter: 800µm, Depth: 400µm] TCI-4860-903SP-10P Room Temp. 10 plates 930.00 Buy
#904
Product Cat.No. Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish [Diameter: 800µm, Depth: 300µm, No. of Well: 700/dish] 4000-904SP Room Temp. 10 dishes 440.00 Buy
#905
Product Cat.No. Storage PKG Size Price(US$)  
EZSPHERE™ 35 mm Dish [Diameter: 1,400µm, Depth: 600µm, No. of Well: 260/dish] 4000-905SP Room Temp. 10 dishes 440.00 Buy
Variety Pack
Product Cat.No. Storage PKG Size Price(US$)  
EZSPHERE™ Variety Pack (35 mm dish), 6ea / pack 4000-9VP Room Temp. 1 pack 210.00 Buy