Nacalai USA - Innovations for Life Sciences

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ISOkine - Plant-based Growth Factors & Cytokines

Animal-component-free (ACF) / Endotoxin-free

ISOkine are highly purified, animal-component-free (ACF), easily scalable, endotoxin-free growth factors and cytokines produced as recombinant proteins in our barley plants. Renewable geothermal energy is used in their production, leaving a low carbon footprint and making them environmental-friendly. 

Features: 

  • Anmial-Free Contaminant-Free: Entirely free of serums, antibiotics, endotoxins, viruses, andunwanted cytokines.
  • Endotoxin-Free Assurance: Industry-leading endotoxin levels [<0.005 ng per μg (0.05 EU/μg)] prevent unwanted cell responses

Applications:

  • Stem Cell Research: Designed for expansion, differentiation, and characterization of human immune cells
  • Regenerative Medicine: Premium-grade Growth Factors compliant with regulatory guidelines for ancillary materials in clinical manufacturing processes.
  • Diagnostics: Reliable immunoassay controls for robust and accurate diagnostic applications.
  • Research: Ideal for preclinical studies, bridging to GMP- grade products for clinical applications.

 

Plant-based technology

ORF Genetics have developed an unique expression system which uses barley grain as a vehicle for production of recombinant human and animal proteins.​

The targeted gene is cloned into ORF’s expression vector. The immature barley embryos undergo tissue culture on a optimized medium.  Then the plantlets are transferred into a small pot in a specialized chamber with controlled settings. Following a growth period, the plantlets are transferred into hydroponic conveyer belt cultivation system until they are ready to harvest. 

Biological Activity

Biological activity of ISOKine is at least comparable but generally higher than other commercially available growth factors and cytokines, reflecting the proper and efficient eukaryotic folding and glycosylation pattern of proteins produced in plant cells.

Each batch of ISOkine PREMIUM growth factors and cytokines is tested for biological activity by an independent third-party research organization, and all bioactivity data is provided with each batch for every product.

Product list

ISOKine Growth-Factors and Cytokines. 

FAQ

- Do ISOkine PREMIUM growth factors and cytokines contain animal derived substances?

As they are produced in barley seeds, they do not come in contact with any animal derived substances throughout their production cycle and are fully Animal-Component-Free (ACF).

- Do ISOkine PREMIUM growth factors and cytokines expose the threat of contamination with bacteria, viruses, or prions?

They are produced in barley and purified in laboratories that only process barley-derived proteins. Therefore, any contact of the products with bacteria, virus, or prion-derived contaminants can be excluded.

- Can ISOKine be used for therapeutics or clinical testing?

ISOKine should only be used for research purposes. 

- For how long are ISOKine products stable? How should I store the proteins?

The lyophilized protein is stable at room temperature for several weeks. However, we recommend to stored it at -20°C. Reconstituted proteins should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. If properly stored, our growth factors are stable for at least 3 years from product release.

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Flyer: "ISOkine

Ordering Information

Product Description Storage Cat.No. PKG Size Price  
EGF (Epidermal Growth Factor) Human -20°C IK0100-10UG 10 μg 29.00
Buy
EGF (Epidermal Growth Factor) Human -20°C IK0100-50UG 50 μg 58.00
Buy
EGF (Epidermal Growth Factor) Human -20°C IK0100-100UG 100 μg 81.00
Buy
EGF (Epidermal Growth Factor) Human -20°C IK0100-1000UG 1000 μg 229.00
Buy
FGFb/FGF2 (Fibroblast growth factor basic (bFGF)/FGF2) Human -20°C IK0200-10UG 10 μg 92.00
Buy
FGFb/FGF2 (Fibroblast growth factor basic (bFGF)/FGF2) Human -20°C IK0200-50UG 50 μg 172.00
Buy
FGFb/FGF2 (Fibroblast growth factor basic (bFGF)/FGF2) Human -20°C IK0200-100UG 100 μg 229.00
Buy
FGFb/FGF2 (Fibroblast growth factor basic (bFGF)/FGF2) Human -20°C IK0200-1000UG 1000 μg 743.00
Buy
IGF-1, LR3 (Insulin-like Growth Factor 1 LR3) Human -20°C IK0300-10UG 10 μg 29.00
Buy
IGF-1, LR3 (Insulin-like Growth Factor 1 LR3) Human -20°C IK0300-50UG 50 μg 58.00
Buy
IGF-1, LR3 (Insulin-like Growth Factor 1 LR3) Human -20°C IK0300-100UG 100 μg 81.00
Buy
IGF-1, LR3 (Insulin-like Growth Factor 1 LR3) Human -20°C IK0300-1000UG 1000 μg 229.00
Buy
IL-6 (Interleukin-6) Human -20°C IK0400-10UG 10 μg 86.00
Buy
IL-6 (Interleukin-6) Human -20°C IK0400-50UG 50 μg 344.00
Buy
IL-6 (Interleukin-6) Human -20°C IK0400-100UG 100 μg 514.00
Buy
IL-6 (Interleukin-6) Human -20°C IK0400-1000UG 1000 μg 2,288.00
Buy
VEGF165 (Vascular endothelial growth factor, variant 165) Human -20°C IK0500-10UG 10 μg 143.00
Buy
VEGF165 (Vascular endothelial growth factor, variant 165) Human -20°C IK0500-50UG 50 μg 402.00
Buy
VEGF165 (Vascular endothelial growth factor, variant 165) Human -20°C IK0500-100UG 100 μg 572.00
Buy
VEGF165 (Vascular endothelial growth factor, variant 165) Human -20°C IK0500-1000UG 1000 μg 2,855.00
Buy
KGF/FGF7 (Keratinocyte Growth Factor (KGF, FGF 7, HBGF 7)) Human -20°C IK0600-10UG 10 μg 172.00
Buy
KGF/FGF7 (Keratinocyte Growth Factor (KGF, FGF 7, HBGF 7)) Human -20°C IK0600-50UG 50 μg 571.00
Buy
KGF/FGF7 (Keratinocyte Growth Factor (KGF, FGF 7, HBGF 7)) Human -20°C IK0600-100UG 100 μg 858.00
Buy
KGF/FGF7 (Keratinocyte Growth Factor (KGF, FGF 7, HBGF 7)) Human -20°C IK0600-1000UG 1000 μg 3,427.00
Buy
LIF (Leukemia inhibitory factor, human) Human -20°C IK0700-10UG 10 μg 115.00
Buy
LIF (Leukemia inhibitory factor, human) Human -20°C IK0700-50UG 50 μg 315.00
Buy
LIF (Leukemia inhibitory factor, human) Human -20°C IK0700-100UG 100 μg 514.00
Buy
LIF (Leukemia inhibitory factor, human) Human -20°C IK0700-1000UG 1000 μg 2,162.00
Buy
LIF (Leukemia inhibitory factor, mouse) Mouse -20°C IK0701-10UG 10 μg 116.00
Buy
LIF (Leukemia inhibitory factor, mouse) Mouse -20°C IK0701-50UG 50 μg 352.00
Buy
LIF (Leukemia inhibitory factor, mouse) Mouse -20°C IK0701-100UG 100 μg 589.00
Buy
LIF (Leukemia inhibitory factor, mouse) Mouse -20°C IK0701-1000UG 1000 μg 2,288.00
Buy
GDNF (Glial cell line derived neurotrophic factor) Human -20°C IK0900-10UG 10 μg 171.00
Buy
GDNF (Glial cell line derived neurotrophic factor) Human -20°C IK0900-50UG 50 μg 402.00
Buy
GDNF (Glial cell line derived neurotrophic factor) Human -20°C IK0900-100UG 100 μg 572.00
Buy
GDNF (Glial cell line derived neurotrophic factor) Human -20°C IK0900-1000UG 1000 μg 3,146.00
Buy
PDGFbb (Platelet-Derived Growth Factor) Human -20°C IK1000-10UG 10 μg 116.00
Buy
PDGFbb (Platelet-Derived Growth Factor) Human -20°C IK1000-50UG 50 μg 402.00
Buy
PDGFbb (Platelet-Derived Growth Factor) Human -20°C IK1000-100UG 100 μg 572.00
Buy
PDGFbb (Platelet-Derived Growth Factor) Human -20°C IK1000-1000UG 1000 μg 2,860.00
Buy
Flt3-ligand (FMS related tyrosine kinase 3 ligand, flk-2 ligand) Human -20°C IK1700-10UG 10 μg 172.00
Buy
Flt3-ligand (FMS related tyrosine kinase 3 ligand, flk-2 ligand) Human -20°C IK1700-50UG 50 μg 372.00
Buy
Flt3-ligand (FMS related tyrosine kinase 3 ligand, flk-2 ligand) Human -20°C IK1700-100UG 100 μg 686.00
Buy
Flt3-ligand (FMS related tyrosine kinase 3 ligand, flk-2 ligand) Human -20°C IK1700-1000UG 1000 μg 3,564.00
Buy
G-CSF (Granulocyte Colony-Stimulating Factor) Human -20°C IK1800-10UG 10 μg 172.00
Buy
G-CSF (Granulocyte Colony-Stimulating Factor) Human -20°C IK1800-50UG 50 μg 372.00
Buy
G-CSF (Granulocyte Colony-Stimulating Factor) Human -20°C IK1800-100UG 100 μg 686.00
Buy
G-CSF (Granulocyte Colony-Stimulating Factor) Human -20°C IK1800-1000UG 1000 μg 3,564.00
Buy
IFN gamma (Interferon gamma (IFNG or IFN-γ)) Human -20°C IK2000-10UG 10 μg 58.00
Buy
IFN gamma (Interferon gamma (IFNG or IFN-γ)) Human -20°C IK2000-50UG 50 μg 115.00
Buy
IFN gamma (Interferon gamma (IFNG or IFN-γ)) Human -20°C IK2000-100UG 100 μg 172.00
Buy
IFN gamma (Interferon gamma (IFNG or IFN-γ)) Human -20°C IK2000-1000UG 1000 μg 1,089.00
Buy
IL-2 (Interleukin-2) Human -20°C IK2100-10UG 10 μg 81.00
Buy
IL-2 (Interleukin-2) Human -20°C IK2100-50UG 50 μg 154.00
Buy
IL-2 (Interleukin-2) Human -20°C IK2100-100UG 100 μg 198.00
Buy
IL-2 (Interleukin-2) Human -20°C IK2100-1000UG 1000 μg 770.00
Buy
IL-3 (Interleukin-3, Hematopoietic growth factor) Human -20°C IK2200-10UG 10 μg 189.00
Buy
IL-3 (Interleukin-3, Hematopoietic growth factor) Human -20°C IK2200-50UG 50 μg 315.00
Buy
IL-3 (Interleukin-3, Hematopoietic growth factor) Human -20°C IK2200-100UG 100 μg 543.00
Buy
IL-3 (Interleukin-3, Hematopoietic growth factor) Human -20°C IK2200-1000UG 1000 μg 1,716.00
Buy
IL-4 (Interleukin-4, B cell stimulatory factor) Human -20°C IK2300-10UG 10 μg 115.00
Buy
IL-4 (Interleukin-4, B cell stimulatory factor) Human -20°C IK2300-50UG 50 μg 344.00
Buy
IL-4 (Interleukin-4, B cell stimulatory factor) Human -20°C IK2300-100UG 100 μg 571.00
Buy
IL-4 (Interleukin-4, B cell stimulatory factor) Human -20°C IK2300-1000UG 1000 μg 2,574.00
Buy
M-CSF (Macrophage colony stimulating factor) Human -20°C IK2400-10UG 10 μg 172.00
Buy
M-CSF (Macrophage colony stimulating factor) Human -20°C IK2400-50UG 50 μg 401.00
Buy
M-CSF (Macrophage colony stimulating factor) Human -20°C IK2400-100UG 100 μg 600.00
Buy
M-CSF (Macrophage colony stimulating factor) Human -20°C IK2400-1000UG 1000 μg 3,146.00
Buy
SCF (Stem Cell Factor (SCF, also known as c-kit ligand) Human -20°C IK2500-10UG 10 μg 115.00
Buy
SCF (Stem Cell Factor (SCF, also known as c-kit ligand) Human -20°C IK2500-50UG 50 μg 286.00
Buy
SCF (Stem Cell Factor (SCF, also known as c-kit ligand) Human -20°C IK2500-100UG 100 μg 457.00
Buy
SCF (Stem Cell Factor (SCF, also known as c-kit ligand) Human -20°C IK2500-1000UG 1000 μg 1,430.00
Buy
SCF (Stem Cell Factor (SCF, also known as c-kit ligand, mouse) Mouse -20°C IK2501-10UG 10 μg 198.00
Buy
SCF (Stem Cell Factor (SCF, also known as c-kit ligand, mouse) Mouse -20°C IK2501-50UG 50 μg 341.00
Buy
SCF (Stem Cell Factor (SCF, also known as c-kit ligand, mouse) Mouse -20°C IK2501-100UG 100 μg 572.00
Buy
SCF (Stem Cell Factor (SCF, also known as c-kit ligand, mouse) Mouse -20°C IK2501-1000UG 1000 μg 2,288.00
Buy
TNF alpha (Tumor necrosis factor, alpha) Human -20°C IK2600-10UG 10 μg 58.00
Buy
TNF alpha (Tumor necrosis factor, alpha) Human -20°C IK2600-50UG 50 μg 171.00
Buy
TNF alpha (Tumor necrosis factor, alpha) Human -20°C IK2600-100UG 100 μg 231.00
Buy
TNF alpha (Tumor necrosis factor, alpha) Human -20°C IK2600-1000UG 1000 μg 974.00
Buy
GM-CSF (Granulocyte-macrophage colony-stimulating factor) Human -20°C IK1900-10UG 10 μg 172.00
Buy
GM-CSF (Granulocyte-macrophage colony-stimulating factor) Human -20°C IK1900-50UG 50 μg 401.00
Buy
GM-CSF (Granulocyte-macrophage colony-stimulating factor) Human -20°C IK1900-100UG 100 μg 600.00
Buy
GM-CSF (Granulocyte-macrophage colony-stimulating factor) Human -20°C IK1900-1000UG 1000 μg 3,828.00
Buy

Shipping at room temperature