Bullet PAGE One Precast Gel can produce an excellent separation image with the shortest electrophoresis.

Product Name	Bullet PAGE One	Company A	Company B
Gel %	5 - 15%	4 - 15%	4 - 12%
Product No.	13079-84	-	-
Electrophoresis Time	10 min.	33 min.	50 min.
Electrophoresis Images

Electrophoresis condition

Bullet PAGE One Precast Gel indicates obviously higher protein transfer efficiency than company A's, even though blotting time was only 10 min.

Sample
① Protein Ladder One(Triple-color)(Product No. ： 09547-74)　
② Protein Markers(3x) 2 µl added
④ Protein Markers(1x) 2 µl added
⑤ Protein Markers(1/3) 2 µl added

Transfer
Use Semi ‐ dry Blotting Buffer Solution for Western Blotting (Product No.: 30650-31)
(Transfer time : 10 min. and 20 min. 10V)

PVDF membrane staining
Stain PVDF membrane with CBB Stain One (Product No. : 04543).

Coomassie brilliant blue staining and western blotting

The western blot is a composite image using different exposure times for each protein. This data shows that Bullet PAGE One Precast Gel is compatible with CBB staining and western blot techniques.

Data courtesy of professor Tatsuya Moriyama, Faculty of Agriculture, Kinki University

Instruction: Bullet PAGE Plus Precast Gel

• Check the electrophoresis device (compatible glass plate size, etc.) and output at power supply (maximum voltage, etc.). We recommend the WEP-MN Vertical Electrophoresis Cell which is specifically designed for running the Bullet PAGE One Precast Gels and Bullet PAGE Plus Precast Gels. The cell is made of durable polycarbonate and tolerates high voltage running conditions for ultra-high speed electrophoresis.
• Electrophoresis should run below 400V of constant voltage.
• Electrophoresis time will be influenced by the temperature of the running buffer and the concentration of acrylamide in the gel (1%).
• The recommended application volume is below the half of well volume. (13-well type: below 20 µl, 17-well type: below 14 µl).

1. Asano, Kosuke, et al. "An insulin-inducible transcription factor, SHARP-1, represses transcription of the SIRT1 longevity gene." Biochemistry and Biophysics Reports 22 (2020): 100743.