COSMOCORE 2.6PBr
Features
- Separate hydrophilic compounds in reversed-phase conditions
- Retain hydrophilic compounds longer than C18
- Greater sample loading capacity than HILIC
- High performance similar to sub-2 µm particles with lower back pressure
Suitable Samples
- Hydrophilic compounds
- Nucleic acids and derivatives
- Surfactants
- Glycosides
- Peptides
» Information on COSMOSIL 5PBr (fully porous particles)
| Packing Material | COSMOCORE 2.6PBr |
|---|---|
| Silica gel | Core-shell type silica gel |
| Average particle size | 2.6 µm |
| Average pore size | approx. 90Å |
| Specific surface area | approx. 150m2/g |
| Stationary phase | Pentabromobenzyl Group |
| Endcapping treatment | Yes |
| Usable pH range | 2 - 7.5 |
| Maximum puressure | 60MPa |
>> Click here to COSMOCORE Development Kit
Separation of Hydrophilic Compounds (low retention on C18)
COSMOSIL PBr retains hydrophilic compounds stronger than C18 columns under the same reversed-phase conditions.
Nucleic Acid Metabolites
Malonyl CoA, CoA, Acetyl CoA
| Column: | COSMOCORE ** |
| Column size: | 2.1mmI.D.-100mm |
| Mobile phase: | Acetonitrile/ 20mmol/l Phosphate buffer(pH7.0) = 5/95 |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV260nm |
| Sample: | 1; Malonyl CoA (0.5mg/ml) 2; CoA (0.5mg/ml) 3; Acetyl CoA (0.5mg/ml) |
| Inj. Vol: | 1.0µl |
Water-Soluble Vitamins
| Column: | COSMOCORE 2.6PBr |
| Column size: | 2.1mmI.D.-150mm |
| Mobile phase: | A; 20mmol/l Phosphate buffer(pH2.5) B; Methanol/ 20mmol/l P hosphate buffer(pH2.5) = 60/40 B conc. 0% (0-1min), 0→80% (1→5min), 80→100% (5→9min) |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV220nm |
| Sample: | 1; Vitamin B1 [Thiamine] (0.25mg/ml) 2; Vitamin C [Ascorbic Acid] (1.0mg/ml) 3; Vitamin B3 [Nicotinic Acid] ( 0.04mg/ml) 4; Vitamin B3 [Nicotinamide] ( 0.06mg/ml) 5; Vitamin B6 [Pyridoxine] (0.25mg/ml) 6; Vitamin B5 [Pantothenic Acid] (2.0mg/ml) 7; Vitamin B9 [Folic Acid] (0.2mg/ml) 8; Vitamin B7 [Biotin] (3.0mg/ml) 9; Vitamin B12 [Cyanocobalamin] (0.25mg/ml) 10; Vitamin B2 [Riboflavin] (0.2mg/ml) |
| Inj. Vol: | 1.25µl |
Separation of Hydrophilic Compounds (compounds with similar hydrophobicity)
COSMOCORE 2.6PBr can separate compounds with similar hydrophobicity, utilizing several kinds of molecular interactions, including dispersion force generated by the bromine atoms.
ATP, ADP, AMP
| Column: | COSMOCORE ** |
| Column size: | 2.1mmI.D.-100mm |
| Mobile phase: | 20mmol/l Phosphate buffer(pH7.0) |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV260nm |
| Sample: | 1; ATP (0.5mg/ml) 2; ADP (0.8mg/ml) 3; AMP (0.8mg/ml) |
| Inj. Vol: | 0.5µl |
Vitamin B3 (Nicotinic Acid, Nicotinamide)
| Column: | COSMOCORE 2.6PBr |
| Column size: | 2.1mmI.D.-150mm |
| Mobile phase: | 10mmol/l Phosphate buffer(pH7.0) |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV260nm |
| Sample: | 1; Nicotinic Acid (0.4mg/ml) 2; Nicotinamide (0.6mg/ml) |
| Inj. Vol: | 0.5µl |
Thiamine Pyrophosphate, Thiamine
| Column: | COSMOCORE 2.6PBr |
| Column size: | 2.1mmI.D.-150mm |
| Mobile phase: | 100mmol/l Phosphate buffer(pH2.5) |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV245nm |
| Sample: | 1; Thiamine Pyrophosphate (0.4mg/ml) 2; Thiamine (0.4mg/ml) |
| Inj. Vol: | 0.5µl |
UDP Glycosides
| Column: | COSMOCORE 2.6PBr |
| Column size: | 2.1mmI.D.-150mm |
| Mobile phase: | 100mmol/l Phosphate buffer(pH7.0) |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV260nm |
| Sample: | 1; UDP (0.8mg/ml) 2; UDP-Galactose (0.8mg/ml) 3; UDP-Glucose (0.8mg/ml) |
| Inj. Vol: | 0.5µl |
Arbutin and Hydroquinone
| Column: | COSMOCORE ** |
| Column size: | 2.1mmI.D.-100mm |
| Mobile phase: | Acetonitrile/ 20mmol/l Phosphate buffer(pH2.5) |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV290nm |
| Sample: | 1; Arbutin (1.33mg/ml) 2; Hydroquinone (0.67mg/ml) |
| Inj. Vol: | 0.75µl |
Separation Mechanism
- Dispersion force (instantaneous dipole–induced dipole force)
London dispersion force is a weak intermolecular force that results from dipoles temporarily induced by random unsymmetrical electron positions in two adjacent atoms, also known as instantaneous dipole–induced dipole force. It is present in all molecules regardless of polarity. Compounds with high polarizability have stronger dispersion force. - Compounds with stronger dispersion force
- Larger and heavier molecules
- Molecules with larger and heavier atoms (e.g. from weakest to strongest in halogens, F2, Cl2, Br2, and I2)
- Molecules with delocalized electrons and resonance (e.g. aromatic compounds)
⇓
COSMOSIL PBr column is packed with pentabromobenzyl-bonded silica that enables separation by dispersion force interaction.
Difference between Methanol and Acetonitrile Mobile Phases
Comparison of Separation Ability
Acetonitrile is frequently used in HPLC to reduce backpressure. However, the π electrons in acetonitrile interfere with the dispersion force interaction between the sample and the stationary phase. Therefore, we recommend using methanol as the organic solvent.
| Column: | COSMOCORE 2.6PBr |
| Column size: | 2.1mmI.D.-100mm |
| Mobile phase: | ** |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV210nm |
| Sample: | 1; Stevioside (5.0mg/ml) 2; RebaudiosideA |
| Inj. Vol: | 1.0µl |
Comparison of Pressure
In general, methanol/water mobile phases exhibit significantly higher pressure than acetonitrile/water. However, the pressure never exceeded 30 MPa in our test of a 2.1 mm x 100 mm column. Since COSMOSORE 2.6PBr can withstand 60 MPa, methanol-based mobile phases are usable even with UHPLC.
| Column: | COSMOCORE 2.6PBr (2.1mmI.D.-100mm) |
| Mobile phase: | See graph |
| Temperature: | 40°C |
| Flow rate: | 0.4 ml/min |
Comparison to HILIC
Both columns are suitable for analysis of hydrophilic compounds, but they have different properties.
| PBr | HILIC | |
|---|---|---|
| Separation Mode | Reversed Phase | Separation Mode |
| Features |
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COSMOSIL Application
Ordering Information
Column
| Product | Cat.No. | Quantity | Price(US$) | |
|---|---|---|---|---|
| COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 30mm | 13692-21 | 1 pkg | 630.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 50mm | 13693-11 | 1 pkg | 670.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 75mm | 13694-01 | 1 pkg | 735.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 100mm | 13695-91 | 1 pkg | 760.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 150mm | 13697-71 | 1 pkg | 775.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 30mm | 13698-61 | 1 pkg | 630.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 50mm | 13699-51 | 1 pkg | 670.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 75mm | 13700-01 | 1 pkg | 735.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 100mm | 13701-91 | 1 pkg | 760.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 150mm | 13703-71 | 1 pkg | 775.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 30mm | 13705-51 | 1 pkg | 630.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 50mm | 13712-51 | 1 pkg | 670.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 75mm | 13714-31 | 1 pkg | 735.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 100mm | 13715-21 | 1 pkg | 760.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 150mm | 13719-81 | 1 pkg | 775.00 | Buy |
| COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 250mm | 13734-71 | 1 pkg | 975.00 | Buy |
Prefilter
| Product | Cat.No. | Quantity | Price(US$) | |
|---|---|---|---|---|
| U-Fil UHPLC-compatible prefilter | 12571-31 | 1 pkg | 160.00 | Buy |