» Information on COSMOSIL 5PBr (fully porous particles)
Packing Material | COSMOCORE 2.6PBr |
---|---|
Silica gel | Core-shell type silica gel |
Average particle size | 2.6 µm |
Average pore size | approx. 90Å |
Specific surface area | approx. 150m2/g |
Stationary phase | Pentabromobenzyl Group |
Endcapping treatment | Yes |
Usable pH range | 2 - 7.5 |
Maximum puressure | 60MPa |
>> Click here to COSMOCORE Development Kit
COSMOSIL PBr retains hydrophilic compounds stronger than C18 columns under the same reversed-phase conditions.
Nucleic Acid Metabolites
Malonyl CoA, CoA, Acetyl CoA
Column: | COSMOCORE ** |
Column size: | 2.1mmI.D.-100mm |
Mobile phase: | Acetonitrile/ 20mmol/l Phosphate buffer(pH7.0) = 5/95 |
Flow rate: | 0.4 ml/min |
Temperature: | 40°C |
Detection: | UV260nm |
Sample: | 1; Malonyl CoA (0.5mg/ml) 2; CoA (0.5mg/ml) 3; Acetyl CoA (0.5mg/ml) |
Inj. Vol: | 1.0µl |
Water-Soluble Vitamins
Column: | COSMOCORE 2.6PBr |
Column size: | 2.1mmI.D.-150mm |
Mobile phase: | A; 20mmol/l Phosphate buffer(pH2.5) B; Methanol/ 20mmol/l P hosphate buffer(pH2.5) = 60/40 B conc. 0% (0-1min), 0→80% (1→5min), 80→100% (5→9min) |
Flow rate: | 0.4 ml/min |
Temperature: | 40°C |
Detection: | UV220nm |
Sample: | 1; Vitamin B1 [Thiamine] (0.25mg/ml) 2; Vitamin C [Ascorbic Acid] (1.0mg/ml) 3; Vitamin B3 [Nicotinic Acid] ( 0.04mg/ml) 4; Vitamin B3 [Nicotinamide] ( 0.06mg/ml) 5; Vitamin B6 [Pyridoxine] (0.25mg/ml) 6; Vitamin B5 [Pantothenic Acid] (2.0mg/ml) 7; Vitamin B9 [Folic Acid] (0.2mg/ml) 8; Vitamin B7 [Biotin] (3.0mg/ml) 9; Vitamin B12 [Cyanocobalamin] (0.25mg/ml) 10; Vitamin B2 [Riboflavin] (0.2mg/ml) |
Inj. Vol: | 1.25µl |
COSMOCORE 2.6PBr can separate compounds with similar hydrophobicity, utilizing several kinds of molecular interactions, including dispersion force generated by the bromine atoms.
ATP, ADP, AMP
Column: | COSMOCORE ** |
Column size: | 2.1mmI.D.-100mm |
Mobile phase: | 20mmol/l Phosphate buffer(pH7.0) |
Flow rate: | 0.4 ml/min |
Temperature: | 40°C |
Detection: | UV260nm |
Sample: | 1; ATP (0.5mg/ml) 2; ADP (0.8mg/ml) 3; AMP (0.8mg/ml) |
Inj. Vol: | 0.5µl |
Vitamin B3 (Nicotinic Acid, Nicotinamide)
Column: | COSMOCORE 2.6PBr |
Column size: | 2.1mmI.D.-150mm |
Mobile phase: | 10mmol/l Phosphate buffer(pH7.0) |
Flow rate: | 0.4 ml/min |
Temperature: | 40°C |
Detection: | UV260nm |
Sample: | 1; Nicotinic Acid (0.4mg/ml) 2; Nicotinamide (0.6mg/ml) |
Inj. Vol: | 0.5µl |
Thiamine Pyrophosphate, Thiamine
Column: | COSMOCORE 2.6PBr |
Column size: | 2.1mmI.D.-150mm |
Mobile phase: | 100mmol/l Phosphate buffer(pH2.5) |
Flow rate: | 0.4 ml/min |
Temperature: | 40°C |
Detection: | UV245nm |
Sample: | 1; Thiamine Pyrophosphate (0.4mg/ml) 2; Thiamine (0.4mg/ml) |
Inj. Vol: | 0.5µl |
UDP Glycosides
Column: | COSMOCORE 2.6PBr |
Column size: | 2.1mmI.D.-150mm |
Mobile phase: | 100mmol/l Phosphate buffer(pH7.0) |
Flow rate: | 0.4 ml/min |
Temperature: | 40°C |
Detection: | UV260nm |
Sample: | 1; UDP (0.8mg/ml) 2; UDP-Galactose (0.8mg/ml) 3; UDP-Glucose (0.8mg/ml) |
Inj. Vol: | 0.5µl |
Arbutin and Hydroquinone
Column: | COSMOCORE ** |
Column size: | 2.1mmI.D.-100mm |
Mobile phase: | Acetonitrile/ 20mmol/l Phosphate buffer(pH2.5) |
Flow rate: | 0.4 ml/min |
Temperature: | 40°C |
Detection: | UV290nm |
Sample: | 1; Arbutin (1.33mg/ml) 2; Hydroquinone (0.67mg/ml) |
Inj. Vol: | 0.75µl |
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COSMOSIL PBr column is packed with pentabromobenzyl-bonded silica that enables separation by dispersion force interaction.
Comparison of Separation Ability
Acetonitrile is frequently used in HPLC to reduce backpressure. However, the π electrons in acetonitrile interfere with the dispersion force interaction between the sample and the stationary phase. Therefore, we recommend using methanol as the organic solvent.
Column: | COSMOCORE 2.6PBr |
Column size: | 2.1mmI.D.-100mm |
Mobile phase: | ** |
Flow rate: | 0.4 ml/min |
Temperature: | 40°C |
Detection: | UV210nm |
Sample: | 1; Stevioside (5.0mg/ml) 2; RebaudiosideA |
Inj. Vol: | 1.0µl |
Comparison of Pressure
In general, methanol/water mobile phases exhibit significantly higher pressure than acetonitrile/water. However, the pressure never exceeded 30 MPa in our test of a 2.1 mm x 100 mm column. Since COSMOSORE 2.6PBr can withstand 60 MPa, methanol-based mobile phases are usable even with UHPLC.
Column: | COSMOCORE 2.6PBr (2.1mmI.D.-100mm) |
Mobile phase: | See graph |
Temperature: | 40°C |
Flow rate: | 0.4 ml/min |
Both columns are suitable for analysis of hydrophilic compounds, but they have different properties.
PBr | HILIC | |
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Separation Mode | Reversed Phase | Separation Mode |
Features |
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Product | Cat.No. | PKG Size | Price(US$) | |
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COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 30mm | 13692-21 | 1 pkg | 635.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 50mm | 13693-11 | 1 pkg | 665.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 75mm | 13694-01 | 1 pkg | 745.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 100mm | 13695-91 | 1 pkg | 760.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 150mm | 13697-71 | 1 pkg | 810.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 30mm | 13698-61 | 1 pkg | 635.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 50mm | 13699-51 | 1 pkg | 665.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 75mm | 13700-01 | 1 pkg | 745.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 100mm | 13701-91 | 1 pkg | 760.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 150mm | 13703-71 | 1 pkg | 810.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 30mm | 13705-51 | 1 pkg | 635.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 50mm | 13712-51 | 1 pkg | 665.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 75mm | 13714-31 | 1 pkg | 745.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 100mm | 13715-21 | 1 pkg | 760.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 150mm | 13719-81 | 1 pkg | 815.00 | Buy |
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 250mm | 13734-71 | 1 pkg | 1,015.00 | Buy |
Product | Cat.No. | PKG Size | Price(US$) | |
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U-Fil UHPLC-compatible prefilter | 12571-31 | 1 pkg | 160.00 | Buy |