COSMOCORE 2.6Cholester
Core-Shell Particle
- Cholesterol-bonded reversed phase core-shell column
- Use under the same condition as C18 columns
- Better selectivity for cis-trans isomers, polyphenols, and natural products
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Comparison with C18
COSMOCORE 2.6Cholester offers improved separation for cis-trans isomers than C18 under typical reversed-phase mobile phase.
| Column size: | 2.1 mmI.D. x 100 mm |
| Mobile phase: | Methanol |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV 280 nm |
| Sample: | 1. Vitamin K2 (0.50 mg/ml) 2. Trans-Vitamin K1 (0.50 mg/ml) 3. Cis-Vitamin K1 |
| Inj. Vol.: | 0.5 µl |
Separation Properties
COSMOCRE 2.6Cholester has the same hydrophobicity as C18. It is not necessary to change the analytical conditions when replacing C18 Columns with COSMOCORE 2.6Cholester.
| Column size: | 2.1 mmI.D. x 100 mm |
| Mobile phase: | Acetonitrile : H2O = 50 : 50 |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV 254 nm |
| Sample: | 1. Uracil 2. Methyl Benzoate 3. Toluene 4. Naphthalene |
| Inj. Vol.: | 0.25 µl |
Molecular Shape Selectivity
COSMOCORE 2.6Cholester has excellent shape slectivity due to its structural rigidity. COSMOCORE 2.6Cholester retains planar Triphenylene longer than Triphenylene stereoscopic o-Terphenyl.
| Column size: | 2.1 mmI.D. x 100 mm |
| Mobile phase: | Acetonitrile : H2O = 70 : 30 |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV 254 nm |
| Sample: | 1. o-Terphenyl (0.25 mg/ml) 2. Triphenylene (0.50 mg/ml) |
| Inj. Vol.: | 0.5 µl |
Scaling Up from Analytical to Preparative Separation
COSMOCORE 2.6Cholestrer has the same functional group as the fully-porous COSMOSIL Cholester 5 µm silica packing material. Therefore, the separation pattern in COSMOCORE 2.6Cholester is maintained when scaling up to preparative separation with 5 µm COSMOSIL Cholester.
| Mobile phase: | Methanol |
| Flow rate: | (2.1 mmI.D.) 0.4 ml/min (4.6 mmI.D.) 1.0 ml/min (10.0 mmI.D.) 5.0 ml/min |
| Temperature: | 40°C |
| Detection: | UV 280 nm |
| Sample: | 1. trans-Vitamin K1 2. cis-Vitamin K1 |
The number of reference for COSMOSIL Cholesterol Bonded Stationary Phase
The number of COSMOSIL Cholester reference has grown to over 100 since its introduction in 2005.
(Reference Search: Google Scholar, as of July 2014)
Figure 1: The number of reference of COSMOSIL Cholester and 2.5Cholester
» Information on COSMOSIL Cholester (5 µm)
» Information on COSMOSIL 2.5Cholester (2.5 µm)
Application: Separation of natural components (Tomato Components)
Lycopene exists in many cis-trans isomers. COSMOCORE 2.6Cholester separates these compounds better than C18.
| Column size: | 3.0 mmI.D. x 50 mm |
| Mobile phase: | (2.6C18) Acetonitrile : H2O = 95 : 5 (2.6Cholester) Acetonitrile |
| Flow rate: | 1.0 ml/min |
| Temperature: | 40°C |
| Detection: | UV 470 nm |
| Sample: | 1. Lycopene 2. β-Carotene |
| Inj. Vol.: | 0.5 µl |
Application: Separation of Catechin
| Column size: | 2.1 mmI.D. x 50 mm |
| Mobile phase: | A; Acetonitrile : 0.1% H3PO4 = 10 : 90 B; Acetonitrile : 0.1% H3PO4 = 40 : 60 B conc. 0 → 100% 3 min Linear gradient |
| Flow rate: | 0.6 ml/min |
| Temperature: | 30°C |
| Detection: | UV 280 nm |
| Sample: | 1. (-)-Gallocatechin[(-)-GC] 2. Caffeine 3. (-)-Epigallocatechin[(-)-EGC] 4. (-)-Catechin[(-)-C] 5. (-)-Epicatechin[(-)-EC] 6. (-)-Epigallocatechin G allate[(-)-EGCg] 7. (-)-Gallocatechin Gallate[(-)-GCg] 8. (-)-Epicatechin Gallate[(-)-ECg] 9. (-)-Catechin Gallat[(-)-Cg] |
Applications
Oligonucleotides
Catechins
Poster: Differentiate Δ9-THC from Δ8-THC by Reversed-Phase Core-Shell HPLC Column
Click here to download (pdf)
Ordering Information
Column
| Product | Cat.No. | PKG Size | Price(US$) | |
|---|---|---|---|---|
| COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 30mm | 12858-91 | 1 pkg | 697.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 50mm | 12859-81 | 1 pkg | 712.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 75mm | 12860-41 | 1 pkg | 798.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 100mm | 12861-31 | 1 pkg | 814.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 150mm | 12862-21 | 1 pkg | 867.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 30mm | 12863-11 | 1 pkg | 697.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 50mm | 12864-01 | 1 pkg | 712.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 75mm | 12866-81 | 1 pkg | 798.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 100mm | 12867-71 | 1 pkg | 814.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 150mm | 12868-61 | 1 pkg | 867.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 30mm | 12869-51 | 1 pkg | 711.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 50mm | 12870-11 | 1 pkg | 712.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 75mm | 12871-01 | 1 pkg | 798.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 100mm | 12872-91 | 1 pkg | 814.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 150mm | 12873-81 | 1 pkg | 873.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 250mm | 12875-61 | 1 pkg | 1,110.00 | Buy |
Prefilter
| Product | Cat.No. | PKG Size | Price(US$) | |
|---|---|---|---|---|
| U-Fil UHPLC-compatible prefilter | 12571-31 | 1 pkg | 160.00 | Buy |