StemFit® Basic Series by Ajinomoto: iPS/ES Cell Culture Media
High-Performance iPS / ES Cell Culture Media for Basic and Clinical Research
StemFit®Basic02, Basic03, and Basic04 are capable of reprogramming, maintaining and differentiating human iPS and ES cells under feeder-free culture conditions.
Features
- Weekend-free feeding
- Enabling single cell cloning
- Providing high colony forming efficiency
- Achieving high cost efficiency
＊Basic04 Complete Type culture media contain 80 ng/ml of basic fibroblast growth factor (bFGF)
Media preparation for Basic02 & Basic03
StemFit®Basic02 and Basic03 are provided frozen as a 2 component set containing “Liquid A” and “Liquid B” and can be stored at below -20 °C until use. Use sterile techniques to prepare StemFit® medium.
- Before use, thaw the frozen medium supplements at room temperature or overnight at 2-8 °C. Do not thaw the frozen supplements at 37 °C, as it accelerates the degradation of medium ingredients.
- Aseptically mix medium components by adding the full volume of “Liquid B” to “Liquid A”. Mix thoroughly. If prepared aseptically, StemFit® is ready for use.
- Upon thawing, StemFit®Basic02 medium may be aseptically aliquoted and stored at below -20 °C. Thawed StemFit® may be stored at 2-8 °C for up to two weeks.
Optional: We recommend storing the medium protected from light - Before use, warm aliquots to room temperature and use immediately.
Easy transition from feeder-dependent to feeder-free culture
Feeder-dependent 201B7 iPSCs were transitioned to feeder-free conditions using respective cell adhesion substrates and seeded in StemFit®.
Superior performance
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Superior growth performance on any matrices
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Superior colony-forming efficiency from a single cell clone
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Single-cell expansion
Experience superior expansion with single-cell passaging and consistent performance with various matrices.
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Top class cost-effectiveness
Weekend-free Feeding Schedules
Skip feeding on weekends for flexible scheduling
Precaution and disclaimer
StemFit®Basic02 is for research use only and is not for diagnostic use, therapeutic use (including clinical research), or as a food. This product contains material derived from human plasma. Therefore, this product should be treated as potentially infectious agents and in accordance with universal handling procedures.
Referred protocols
- Technical information: Movies, Technical tips, Protocols, Publications
- The latest protocol: StemFit with iMatrix-511
- Protocol for general version
- CiRA HP: http://www.cira.kyoto-u.ac.jp/e/research/protocol.html
- Nature Protocols: https://www.ncbi.nlm.nih.gov/pubmed/28005067
Related products
Ordering Information
Basic02
| Product | Cat.No. | Storage | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| StemFit Basic02 | BASIC02 | -20°C | Liquid A: 400 mL / Liquid B: 100 mL | 300.00 | Buy |
Basic03
| Product | Cat.No. | Storage | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| StemFit Basic03 | BASIC03 | -20°C | Liquid A: 400 mL / Liquid B: 100 mL | 600.00 | Buy |
| StemFit Basic03 (6 sets) | BASIC03-6 | -20°C | Liquid A: 400 mL / Liquid B: 100 mL | 3,480.00 | Buy |
| StemFit Basic03 GMP grade | BASIC03GMP | -20°C | Liquid A: 400 mL / Liquid B: 100 mL | 1,135.00 | Buy |
| StemFit Basic03 GMP grade (6 sets) | BASIC03GMP-6 | -20°C | Liquid A: 400 mL / Liquid B: 100 mL | 6,470.00 | Buy |
Basic04
| Product | Cat.No. | Storage | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| StemFit Basic04 | BASIC04 | -20°C | 500 mL | 300.00 | Buy |
| StemFit Basic04 (6 sets) | BASIC04-6 | -20°C | 500 mL | 1,710.00 | Buy |
| StemFit Basic04 Complete Type: Ready-to-use, with bFGF added | BASIC04CT | -20°C | 500 mL | 325.00 | Buy |
| StemFit Basic04 Complete Type: Ready-to-use, with bFGF added (6 sets) | BASIC04CT-6 | -20°C | 500 mL | 1,860.00 | Buy |
The shipping fee for StemFit Basic03 GMP grade will be adjusted according to the quantity of the order. Please refer to your invoice for details.