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Home > Products > Life Sciences > Cell Biology > StemFit®: Chemically-defined media & supplement > StemFit® Basic Series by Ajinomoto: iPS/ES Cell Culture Media

StemFit® Basic Series by Ajinomoto: iPS/ES Cell Culture Media

High-Performance iPS / ES Cell Culture Media for Basic and Clinical Research

 StemFit®Basic02, Basic03, and Basic04 are capable of reprogramming, maintaining and differentiating human iPS and ES cells under feeder-free culture conditions.

Features

  • Weekend-free feeding
  • Enabling single cell cloning
  • Providing high colony forming efficiency
  • Achieving high cost efficiency
＊Basic04 Complete Type culture media contain 80 ng/ml of basic fibroblast growth factor (bFGF)

Media preparation for Basic02 & Basic03

StemFit®Basic02 and Basic03 are provided frozen as a 2 component set containing “Liquid A” and “Liquid B” and can be stored at below -20 °C until use. Use sterile techniques to prepare StemFit® medium.

  1. Before use, thaw the frozen medium supplements at room temperature or overnight at 2-8 °C. Do not thaw the frozen supplements at 37 °C, as it accelerates the degradation of medium ingredients.
  2. Aseptically mix medium components by adding the full volume of “Liquid B” to “Liquid A”. Mix thoroughly. If prepared aseptically, StemFit® is ready for use.
  3. Upon thawing, StemFit®Basic02 medium may be aseptically aliquoted and stored at below -20 °C. Thawed StemFit® may be stored at 2-8 °C for up to two weeks.
    Optional: We recommend storing the medium protected from light
  4. Before use, warm aliquots to room temperature and use immediately.

Easy transition from feeder-dependent to feeder-free culture

Feeder-dependent 201B7 iPSCs were transitioned to feeder-free conditions using respective cell adhesion substrates and seeded in StemFit®.

Superior performance

  • Superior growth performance on any matrices

  • Superior colony-forming efficiency from a single cell clone

  • Single-cell expansion

　　   Experience superior expansion with single-cell passaging and consistent performance with various matrices.

        

  • Top class cost-effectiveness

  　　

Weekend-free Feeding Schedules

Skip feeding on weekends for flexible scheduling

Precaution and disclaimer

StemFit®Basic02 is for research use only and is not for diagnostic use, therapeutic use (including clinical research), or as a food. This product contains material derived from human plasma. Therefore, this product should be treated as potentially infectious agents and in accordance with universal handling procedures.

Referred protocols

Publication

Nakauchi Lab @Stanford University: Nishimura T. et al. Sufficiency for inducible Caspase-9 safety switch in human pluripotent stem cells and disease cells. Gene Ther. 2020 « « NEW!

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Ordering Information

Basic02
Product Cat.No. Storage PKG Size Price(US$)  
StemFit Basic02 BASIC02 -20°C Liquid A: 400 mL / Liquid B: 100 mL 300.00 Buy
Basic03
Product Cat.No. Storage PKG Size Price(US$)  
StemFit Basic03 BASIC03 -20°C Liquid A: 400 mL / Liquid B: 100 mL 600.00 Buy
StemFit Basic03 (6 sets) BASIC03-6 -20°C (Liquid A: 400 mL / Liquid B: 100 mL) x 6 3,480.00 Buy
StemFit Basic03 GMP grade BASIC03GMP -20°C Liquid A: 400 mL / Liquid B: 100 mL 1,135.00 Buy
StemFit Basic03 GMP grade (6 sets) BASIC03GMP-6 -20°C (Liquid A: 400 mL / Liquid B: 100 mL) x 6 6,470.00 Buy
Basic04
Product Cat.No. Storage PKG Size Price(US$)  
StemFit Basic04 BASIC04 -20°C 500 mL 300.00 Buy
StemFit Basic04 (6 sets) BASIC04-6 -20°C 500 mL x 6 1,710.00 Buy
StemFit Basic04 Complete Type: Ready-to-use, with bFGF added BASIC04CT -20°C 500 mL 325.00 Buy
StemFit Basic04 Complete Type: Ready-to-use, with bFGF added (6 sets) BASIC04CT-6 -20°C 500 mL x 6 1,860.00 Buy

The shipping fee for StemFit Basic03 GMP grade will be adjusted according to the quantity of the order. Please refer to your invoice for details.