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StemFit® Basic Series by Ajinomoto: iPS/ES Cell Culture Media

High-Performance iPS / ES Cell Culture Media for Basic and Clinical Research

 StemFit®Basic02, Basic03, and Basic04 are capable of reprogramming, maintaining and differentiating human iPS and ES cells under feeder-free culture conditions.

Features

  • Weekend-free feeding
  • Enabling single cell cloning
  • Providing high colony forming efficiency
  • Achieving high cost efficiency
*Basic04 Complete Type culture media contain 80 ng/ml of basic fibroblast growth factor (bFGF)

Media preparation for Basic02 & Basic03

StemFit®Basic02 and Basic03 are provided frozen as a 2 component set containing “Liquid A” and “Liquid B” and can be stored at below -20 °C until use. Use sterile techniques to prepare StemFit® medium.

  1. Before use, thaw the frozen medium supplements at room temperature or overnight at 2-8 °C. Do not thaw the frozen supplements at 37 °C, as it accelerates the degradation of medium ingredients.
  2. Aseptically mix medium components by adding the full volume of “Liquid B” to “Liquid A”. Mix thoroughly. If prepared aseptically, StemFit® is ready for use.
  3. Upon thawing, StemFit®Basic02 medium may be aseptically aliquoted and stored at below -20 °C. Thawed StemFit® may be stored at 2-8 °C for up to two weeks.
    Optional: We recommend storing the medium protected from light
  4. Before use, warm aliquots to room temperature and use immediately.

Easy transition from feeder-dependent to feeder-free culture

Feeder-dependent 201B7 iPSCs were transitioned to feeder-free conditions using respective cell adhesion substrates and seeded in StemFit®.

Superior performance

  • Superior growth performance on any matrices

  • Superior colony-forming efficiency from a single cell clone

  • Single-cell expansion

     Experience superior expansion with single-cell passaging and consistent performance with various matrices.

        

  • Top class cost-effectiveness

    

Weekend-free Feeding Schedules

Skip feeding on weekends for flexible scheduling

Precaution and disclaimer

StemFit®Basic02 is for research use only and is not for diagnostic use, therapeutic use (including clinical research), or as a food. This product contains material derived from human plasma. Therefore, this product should be treated as potentially infectious agents and in accordance with universal handling procedures.

Related products

Ordering Information

Basic02
Product Cat.No. Storage PKG Size Price(US$)  
StemFit Basic02 BASIC02 -20°C Liquid A: 400 mL / Liquid B: 100 mL 300.00 Buy
Basic03
Product Cat.No. Storage PKG Size Price(US$)  
StemFit Basic03 BASIC03 -20°C Liquid A: 400 mL / Liquid B: 100 mL 600.00 Buy
StemFit Basic03 (6 sets) BASIC03-6 -20°C Liquid A: 400 mL / Liquid B: 100 mL 3,480.00 Buy
StemFit Basic03 GMP grade BASIC03GMP -20°C Liquid A: 400 mL / Liquid B: 100 mL 1,135.00 Buy
StemFit Basic03 GMP grade (6 sets) BASIC03GMP-6 -20°C Liquid A: 400 mL / Liquid B: 100 mL 6,470.00 Buy
Basic04
Product Cat.No. Storage PKG Size Price(US$)  
StemFit Basic04 BASIC04 -20°C 500 mL 300.00 Buy
StemFit Basic04 (6 sets) BASIC04-6 -20°C 500 mL 1,710.00 Buy
StemFit Basic04 Complete Type: Ready-to-use, with bFGF added BASIC04CT -20°C 500 mL 325.00 Buy
StemFit Basic04 Complete Type: Ready-to-use, with bFGF added (6 sets) BASIC04CT-6 -20°C 500 mL 1,860.00 Buy

The shipping fee for StemFit Basic03 GMP grade will be adjusted according to the quantity of the order. Please refer to your invoice for details.