StemFit Basic03 and Basic03 GMP are provided frozen medium and can be stored at below -20 °C until use. Use sterile techniques when using this medium.

1. Before use, thaw the frozen “Liquid A” and “Liquid B” with occasionally mixing at room temperature (15-25 °C). Do not thaw the frozen supplements at 37 °C, as it accelerates the degradation of medium ingredients.
2. Aseptically mix medium components by adding the full volume of “Liquid B” to “Liquid A”. Mix thoroughly. If prepared aseptically, StemFit is ready for use.
3. Upon thawing, StemFit medium may be aseptically aliquoted and stored at below -20 °C. Thawed StemFit may be stored at 2-8 °C for up to two weeks.
   Optional: We recommend storing the medium protected from light
4. Before use, warm aliquots to room temperature and use immediately.

StemFit StemFit Basic04 Complete Type is provided frozen medium and can be stored at below -20 °C until use. Use sterile techniques when using this medium.

1. Before use, thaw the frozen Basic04CT with occasionally mixing at room temperature (15-25 °C). Do not thaw, StemFit Basic04 Complete Type at 37°C, as it accelerates the degradation of medium ingredients.
   If precipitations are observed, keep the bottle at room temperature and dissolve them.
2. Upon thawing, StemFit Basic04 Complete Type may be aseptically aliquoted and stored at below -20 °C. Thawed StemFit Basic04 Complete Type may be stored at 2-8 °C for up to two weeks.
   We recommend storing the medium protected from light.
3. Before use, warm aliquots at room temperature and use immediately.

Feeder-dependent 201B7 iPSCs were transitioned to feeder-free conditions using respective cell adhesion substrates and seeded in StemFit®.

• Superior growth performance on any matrices

• Superior colony-forming efficiency from a single cell clone

• Single-cell expansion

　　   Experience superior expansion with single-cell passaging and consistent performance with various matrices.

• Top class cost-effectiveness

Skip feeding on weekends for flexible scheduling

StemFit Basic03 is for research use only and is not for diagnostic use, therapeutic use (including clinical research), or as a food.

StemFit Basic03 GMP is for research and further manufacturing use only. It is not for diagnostic use, therapeutic use, or as a food.

StemFit Basic04 Complete Type is for research use only and is not intended for diagnostic or therapeutic use.

Culture media preparation

https://youtu.be/zdMnnXyMOec

Passage protocol

https://youtu.be/IrIz7QnTOhg

• Technical information from the manufacturer: Movies, Technical tips, Protocols, Publications
• The latest protocol: StemFit with iMatrix-511
• Protocol for general version
• CiRA HP: http://www.cira.kyoto-u.ac.jp/e/research/protocol.html
• Nature Protocols: https://www.ncbi.nlm.nih.gov/pubmed/28005067

<Basic02>

Nakauchi Lab @Stanford University: Nishimura T. et al. Sufficiency for inducible Caspase-9 safety switch in human pluripotent stem cells and disease cells. Gene Ther. 2020 « « NEW!

<Basic03>

Kaneko Lab @CiRA, Kyoto University: Iriguchi S et al. A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy. Nat Commun, 2021. « « NEW!

• Recombinant Human FGF-basic, Animal-free

• Cytokines & Growth Factors

• iMatrix-511

• StemFit® for Differentiation

• Small molecules for iPS/ES cell research