Cell Processor by Kataoka Corp.: Unwanted cell removal and necessary cell sorting
Laser-induced, Light-Responsive-Polymer-Activated, Cell Killing
Purification of adherent cells at high speed All-in-one tool
Features: 
- High speed observations - Phase contrast observation Φ 35 tiled capture time 60 sec.
- High speed discrimination by AI 0.1 sec/frame
- High speed selection and accurate lethal treatment with laser
High speed observations
Easy handling and high speed processing.
Intuitive operability is realized, and high-speed observation (Φ 35 60seconds) can be done throughout the dish.
Phase contrast microscope 60 sec. (100 seconds with AutoFocus)
Fluorescence 110 sec.
Conditions: Objective lens4x Φ35
Object: iPSC, Fluorescent label rBC2LCN-FITC (FUJIFILM Wako Pure Chemical Corporation)
Clamp creation process by laser Φ 35 Full face
□ 0.4 mm clamp creation time 86 sec.
High speed discrimination
High speed discrimination of unwanted cells using deep learning.
Unwanted cells determination example
High speed discrimination of 0.1 sec. / flame. AI can be applied to any other kind of cells.
Product Specifications
| Product | Specifications |
|---|---|
| Corresponding container | Φ35, 60, 100 |
| Observation function | Phase contrast microscope×2, ×4, ×10 Fluorescence microscope: FITC, Cy5 |
| Chamber | 98.6 F, CO2 5% control |
| Laser spot | Φ40 µm |
| Laser processing speed | ～9.8” / sec (250 mm/sec) |
| Device size | W 36.5” × D 30.3” × H 73.6” |
| Cleaning | Downflow by HEAP filter |
| Other | Built-in sterilizing lamp |
| Utility | AC120V, 60Hz |
Note: This product is for research use only and are not intended for diagnostic or therapeutic use.
High speed selection
Sudden differentiation treatment during iPS cell undifferentiation maintenance culture.
Unwanted cells determination example
Remove unwanted spontaneously differentiated cells with laser. Single layer adhesive cells need not be dissociated and killed at high speed by laser irradiation aimed at the spot.
A dedicated light-responsive polymer coated dish is required. Comparison with FACS: Anaplastic iPSC ratio increase from 88.4 % to 98.1% Object: iPSC, Observation before and after laser processing with objective lens x 4
Reference
Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer
Yohei Hayashi, Junichi Matsumoto, Shohei Kumagai, Kana Morishita, Long Xiang, Yohei Kobori, Seiji Hori, Masami Suzuki, Toshiyuki Kanamori, Kazuhiro Hotta & Kimio Sumaru
Communications Biology volume 1, Article number: 218 (2018)
Ordering Information
| Product | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|
| Cell Processor | CPD-017 | 1 device | - | Inquire |
This product is for research use only and are not intended for diagnostic or therapeutic use.