Nacalai USA - Innovations for Life Sciences

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Cell Processor by Kataoka Corp.: Unwanted cell removal and necessary cell sorting

Laser-induced, Light-Responsive-Polymer-Activated, Cell Killing

Purification of adherent cells at high speed All-in-one tool

Features:                                   

  • High speed observations - Phase contrast observation Φ 35 tiled capture time 60 sec.
  • High speed discrimination by AI 0.1 sec/frame
  • High speed selection and accurate lethal treatment with laser

    

       

High speed observations

Easy handling and high speed processing.

Intuitive operability is realized, and high-speed observation (Φ 35 60seconds) can be done throughout the dish.

 

Phase contrast microscope 60 sec. (100 seconds with AutoFocus)
Fluorescence  110 sec.
Conditions: Objective lens4x Φ35
Object: iPSC, Fluorescent label rBC2LCN-FITC (FUJIFILM Wako Pure Chemical Corporation)
Clamp creation process by laser Φ 35 Full face
□ 0.4 mm clamp creation time 86 sec.

High speed discrimination

High speed discrimination of unwanted cells using deep learning.

Unwanted cells determination example

High speed discrimination of 0.1 sec. / flame. AI can be applied to any other kind of cells.

Product Specifications

ProductSpecifications
Corresponding container Φ35, 60, 100
Observation function Phase contrast microscope×2, ×4, ×10
Fluorescence microscope: FITC, Cy5
Chamber 98.6 F, CO2 5% control
Laser spot Φ40 µm
Laser processing speed ~9.8” / sec (250 mm/sec)
Device size W 36.5” × D 30.3” × H 73.6”
Cleaning Downflow by HEAP filter
Other Built-in sterilizing lamp
Utility AC120V, 60Hz

 

Note: This product is for research use only and are not intended for diagnostic or therapeutic use.

High speed selection

Sudden differentiation treatment during iPS cell undifferentiation maintenance culture.

Unwanted cells determination example

Remove unwanted spontaneously differentiated cells with laser. Single layer adhesive cells need not be dissociated and killed at high speed by laser irradiation aimed at the spot.

A dedicated light-responsive polymer coated dish is required. Comparison with FACS: Anaplastic iPSC ratio increase from 88.4 % to 98.1% Object: iPSC, Observation before and after laser processing with objective lens x 4

 

Reference

Automated adherent cell elimination by a high-speed laser mediated by a light-responsive polymer

Yohei Hayashi, Junichi Matsumoto, Shohei Kumagai, Kana Morishita, Long Xiang, Yohei Kobori, Seiji Hori, Masami Suzuki, Toshiyuki Kanamori, Kazuhiro Hotta & Kimio Sumaru
Communications Biology volume 1, Article number: 218 (2018)

Ordering Information

Product Cat.No. PKG Size Price(US$)  
Cell Processor CPD-017 1 device N/A Inquire

This product is for research use only and are not intended for diagnostic or therapeutic use.