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Instrument Settings for COSMOCORE Columns

Using COSMOCORE columns with a conventional (non-UHPLC) instrument

COSMOCORE columns are designed for use with UHPLC instruments. In addition, due to their low backpressure, they can be used with conventional instruments. However, it is necessary to change the following settings.

Instrument Settings

Detector Response Time

Because UHPLC analyses are done at high flow rates, a slow response time can adversely affect peak shape. We recommend setting the response time to 0.1 sec or less.

Other Instrument Parameters

UHPLC is more vulnerable to the effects of dead volume than conventional chromatography. When using a 2.1 mm I.D. column, please use a semi-micro detector cell, injector, and piping (0.1 mm).

Fittings and Adapters

COSMOCORE columns use the same connectors as Waters UPLC® (UHPLC) columns. This is different from our conventional COSMOSIL columns, which use the conventional Waters HPLC-compatible connectors.
UPLC® is a registered trademark of Waters Corporation.

1. Differences between End Fittings
Connection TypeColumn
InstrumentHPLC No adapter required Adapter required
UHPLC Adapter required No adapter required


Connector Shape
HPLC: Conventional Waters-compatible connector
UHPLC: Waters UPLC®-compatible connector
The length of tubing that extends from the ferrule differs from HPLC to UHPLC.



2. COSMOCORE-Compatible Fittings

1) UHPLC instrument fittings
No adapter needed; just connect as-is.
2) HPLC instrument fittings
An adapter or movable (high-pressure) fitting is required to connect the column. See examples for different fitting types below.
• SUS ferrules (HPLC) fixed on the tubing
The column can be connected by using an adapter (SUS union + tubing fixed to UHPLC length).
• PEEK fittings
PEEK fittings do not fix the length of tubing at the end, so they can be used with both types of column. However, please be cautious of their pressure tolerance.

Ordering Information

Product Size Cat.No. PKG Size Price  
Low & Zero Dead Volume Union Material: SUS / Bore diateter: 0.35 mm P0402 1 pkg - Inquire
COSMOSIL Column Connecting Tube (0.25 mm) 37843-69 1 pkg 65.00 Buy
COSMOSIL Column Connecting Tube (0.1 mm) 12570-41 1 pkg 65.00 Buy
UHPLC-Compatible Prefilter
Product Size Cat.No. PKG Size Price  
U-Fil UHPLC-Compatible Prefilter (IN: UHPLC / OUT: UHPLC) Filter: 0.5 µm / Tubing connecting diameter: 1/16 in. 12571-31 1 set 160.00 Buy
U-Fil UHPLC-Compatible Prefilter (IN: HPLC / OUT: UHPLC) Filter: 0.5 µm / Tubing connecting diameter: 1/16 in. 12572-21 1 set 160.00 Buy
U-Fil Replacement Filter Filter: 0.5 µm / Material: SUS316L 15767-91 5 each 140.00 Buy