Nacalai USA, Inc. | Product | Instrument Settings for COSMOCORE Columns

Instrument Settings for COSMOCORE Columns

Using COSMOCORE columns with a conventional (non-UHPLC) instrument

COSMOCORE columns are designed for use with UHPLC instruments. In addition, due to their low backpressure, they can be used with conventional instruments. However, it is necessary to change the following settings.

Instrument Settings

Detector Response Time

Because UHPLC analyses are done at high flow rates, a slow response time can adversely affect peak shape. We recommend setting the response time to 0.1 sec or less.

Other Instrument Parameters

UHPLC is more vulnerable to the effects of dead volume than conventional chromatography. When using a 2.1 mm I.D. column, please use a semi-micro detector cell, injector, and piping (0.1 mm).

Fittings and Adapters

COSMOCORE columns use the same connectors as Waters UPLC^® (UHPLC) columns. This is different from our conventional COSMOSIL columns, which use the conventional Waters HPLC-compatible connectors.
UPLC^® is a registered trademark of Waters Corporation.

1. Differences between End Fittings

Connection Type		Column
Connection Type		HPLC (COSMOSIL)	UHPLC (COSMOCORE)
Instrument	HPLC	No adapter required	Adapter required
Instrument	UHPLC	Adapter required	No adapter required

	HPLC	UHPLC
Connector Shape

HPLC: Conventional Waters-compatible connector

UHPLC: Waters UPLC^®-compatible connector

The length of tubing that extends from the ferrule differs from HPLC to UHPLC.

2. COSMOCORE-Compatible Fittings

1) UHPLC instrument fittings

No adapter needed; just connect as-is.

2) HPLC instrument fittings

An adapter or movable (high-pressure) fitting is required to connect the column. See examples for different fitting types below.

• SUS ferrules (HPLC) fixed on the tubing

The column can be connected by using an adapter (SUS union + tubing fixed to UHPLC length).

• PEEK fittings

PEEK fittings do not fix the length of tubing at the end, so they can be used with both types of column. However, please be cautious of their pressure tolerance.

Ordering Information

Adapters

Product	Cat.No.	Size	PKG Size	Price(US$)
Low & Zero Dead Volume Union	P0402	Material: SUS / Bore diateter: 0.35 mm	1 pkg	-	Inquire
COSMOSIL Column Connecting Tube (0.25 mm)	37843-69		1 pkg	65.00	Buy
COSMOSIL Column Connecting Tube (0.1 mm)	12570-41		1 pkg	65.00	Buy

UHPLC-Compatible Prefilter

Product	Cat.No.	Size	PKG Size	Price(US$)
U-Fil UHPLC-Compatible Prefilter (IN: UHPLC / OUT: UHPLC)	12571-31	Filter: 0.5 µm / Tubing connecting diameter: 1/16 in.	1 set	160.00	Buy
U-Fil UHPLC-Compatible Prefilter (IN: HPLC / OUT: UHPLC)	12572-21	Filter: 0.5 µm / Tubing connecting diameter: 1/16 in.	1 set	160.00	Buy
U-Fil Replacement Filter	15767-91	Filter: 0.5 µm / Material: SUS316L	5 each	140.00	Buy