Using COSMOCORE columns with a conventional (non-UHPLC) instrument
COSMOCORE columns are designed for use with UHPLC instruments. In addition, due to their low backpressure, they can be used with conventional instruments. However, it is necessary to change the following settings.
Because UHPLC analyses are done at high flow rates, a slow response time can adversely affect peak shape. We recommend setting the response time to 0.1 sec or less.
UHPLC is more vulnerable to the effects of dead volume than conventional chromatography. When using a 2.1 mm I.D. column, please use a semi-micro detector cell, injector, and piping (0.1 mm).
COSMOCORE columns use the same connectors as Waters UPLC® (UHPLC) columns. This is different from our conventional COSMOSIL columns, which use the conventional Waters HPLC-compatible connectors.
UPLC® is a registered trademark of Waters Corporation.
|HPLC (COSMOSIL)||UHPLC (COSMOCORE)|
|Instrument||HPLC||No adapter required||Adapter required|
|UHPLC||Adapter required||No adapter required|
2. COSMOCORE-Compatible Fittings
|Low & Zero Dead Volume Union||P0402||Material: SUS / Bore diateter: 0.35 mm||1 pkg||N/A||Inquire|
|COSMOSIL Column Connecting Tube (0.25 mm)||37843-69||1 pkg||65.00||Buy|
|COSMOSIL Column Connecting Tube (0.1 mm)||12570-41||1 pkg||65.00||Buy|
|U-Fil UHPLC-Compatible Prefilter (IN: UHPLC / OUT: UHPLC)||12571-31||Filter: 0.5 µm / Tubing connecting diameter: 1/16 in.||1 set||160.00||Buy|
|U-Fil UHPLC-Compatible Prefilter (IN: HPLC / OUT: UHPLC)||12572-21||Filter: 0.5 µm / Tubing connecting diameter: 1/16 in.||1 set||160.00||Buy|
|U-Fil Replacement Filter||15767-91||Filter: 0.5 µm / Material: SUS316L||5 each||140.00||Buy|