Nacalai USA - Innovations for Life Sciences

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FG beads®: Line-up

Plain beads / Linker beads (Epoxy beads)

  • Plain beads
For immobilization of DNA with a surface epoxy groups. Purification of a transcription factor and complementary DNA or RNA is one of applications.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Epoxy groups
Amounts of the functional groups Approx. 1μmol/mg

 

  • Linker beads (Epoxy beads)
It is difficult for the OH groups to immobilize it to beads in reactivity being low, but phenol groups can be immobilized on epoxy beads under an alkaline condition because reactivity is high. Because epoxy beads produce almost no non-specific protein adsorption, it is not necessary to mask the remaining epoxy groups.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Epoxy groups
Amounts of the functional groups 200-300 nmol/mg

NH2 beads / OH beads

  • NH2 beads
Compounds with COOH groups are immobilized on NH2 beads by covalent bond. But it is necessary to let a ligand activate with NHS ester.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups NH2 groups
Amounts of the functional groups 200-300 nmol/mg

 

  • OH beads
When the reactivity of the COOH groups is high, compounds with COOH groups are immobilized on OH beads by covalent bond. Because OH beads produce almost no non-specific protein adsorption, it is not necessary to mask the remaining OH groups.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups OH groups
Amounts of the functional groups 200-300 nmol/mg

NHS beads / COOH beads

  • NHS beads
Compounds with NH2 groups are immobilized on COOH beads by covalent bond easily. Therefore we can purify target protein at high purity from samples such as the cell extract. And, because antibodies or proteins with NH2 groups are immobilized easily, we can use it for immunoprecipitation and cell separation.
 
Storage buffer isopropyl alcohol
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups NHS groups
Amounts of the functional groups Approx. 1μmol/mg

 

  • COOH beads
Compounds, antibodies and proteins with NH2 groups are immobilized on COOH beads by covalent bond. We can use it in wide application such as the chemical biology or cell separation.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups COOH groups
Amounts of the functional groups 200-300 nmol/mg

Azide beads / Alkyne beads

  • Azide beads
Compounds with alkyne are immobilized on azide beads easily by using a click chemistry reaction. When a compound has plural one functional group, we can immobilize the compound which we introduced an alkyne into on azide beads regiospecifically.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Azide groups
Amounts of the functional groups 70-140 nmol/mg

 

  • Alkyne beads
Compounds with azide groups are immobilized on alkyne beads easily by using a click chemistry reaction. When a compound has plural one functional group, we can immobilize the compound which we introduced an azide group into on alkyne beads regiospecifically.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Alkyne groups
Amounts of the functional groups 70-140 nmol/mg

Ts beads

  • Ts beads
His tagged proteins can be immobilized to Ts beads selectively. This shows superiority for elucidation of the protein-protein interaction.
 
Storage buffer 1mM HCl
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Ts groups
Amounts of the functional groups 200-300 nmol/mg

Protein A beads / Protein G beads / Streptavidin beads / NeutrAvidin beads

  • Protein A beads
Protein A beads are combined with antibody (IgG) mainly. We can use it for the immunoprecipitation experiment using an antigen - antibody reaction or the antibody purification.
 
Storage buffer 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10% glycerol
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Protein A
Capacity >35 ug Human IgG/mg of beads

 

  • Protein G beads
Protein G beads are combined with antibody (IgG) mainly. We can use it for the immunoprecipitation experiment using an antigen - antibody reaction or the antibody purification.
 
Storage buffer 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Protein G
Capacity >35 ug Human IgG/mg of beads

 

  • Streptavidin beads
Biotinylated compounds and biotin-labeled antibody or DNA can be immobolized on Streptavidin beads, and we can use it in wide application. In addition, we can use Streptavidin beads for the separation by biotin-labeled cell.
 
Storage buffer 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Streptavidin
Capacity >3 ug biotinylated BSA/mg of beads

 

  • NeutrAvidin beads
Biotinylated compounds and biotin-labeled antibody or DNA can be immobolized on NeutrAvidin beads, and we can use it in wide application.
 
Storage buffer 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups NeutrAvidin
Capacity >1.5 ug biotinylated BSA/mg of beads

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Plain beads TAS8848N1010-10MG 4°C 10 mg 175.00 Buy
Plain beads TAS8848N1010-20MG 4°C 20 mg 250.00 Buy
Linker beads (Epoxy beads) TAS8848N1110-5MG 4°C 5 mg 155.00 Buy
Linker beads (Epoxy beads) TAS8848N1110-20MG 4°C 20 mg 420.00 Buy
OH beads TAS8848N1120-5MG 4°C 5 mg 230.00 Buy
OH beads TAS8848N1120-20MG 4°C 20 mg 555.00 Buy
NH2 beads TAS8848N1130-5MG 4°C 5 mg 230.00 Buy
NH2 beads TAS8848N1130-20MG 4°C 20 mg 555.00 Buy
COOH beads TAS8848N1140-5MG 4°C 5 mg 230.00 Buy
COOH beads TAS8848N1140-20MG 4°C 20 mg 555.00 Buy
NHS beads TAS8848N1141-5MG -20°C 5 mg 230.00 Buy
NHS beads TAS8848N1141-10MG -20°C 10 mg 400.00 Buy
NHS beads TAS8848N1141-20MG -20°C 20 mg 690.00 Buy
Ts beads TAS8848N1150-5MG 4°C 5 mg 230.00 Buy
Ts beads TAS8848N1150-20MG 4°C 20 mg 555.00 Buy
Azide beads TAS8848N1160-5MG 4°C 5 mg 400.00 Buy
Azide beads TAS8848N1160-10MG 4°C 10 mg 730.00 Buy
Azide beads TAS8848N1160-20MG 4°C 20 mg 1,300.00 Buy
Alkyne beads TAS8848N1161-5MG 4°C 5 mg 400.00 Buy
Alkyne beads TAS8848N1161-10MG 4°C 10 mg 730.00 Buy
Alkyne beads TAS8848N1161-20MG 4°C 20 mg 1,300.00 Buy
HM-Streptavidin beads TAB8848N3170-5MG 4°C 5 mg 400.00 Buy
HM-Streptavidin beads TAB8848N3170-10MG 4°C 10 mg 730.00 Buy
HM-Streptavidin beads TAB8848N3170-20MG 4°C 20 mg 1,300.00 Buy
HM-NeutrAvidin beads TAB8848N3171-5MG 4°C 5 mg 400.00 Buy
HM-NeutrAvidin beads TAB8848N3171-10MG 4°C 10 mg 730.00 Buy
HM-NeutrAvidin beads TAB8848N3171-20MG 4°C 20 mg 1,300.00 Buy
HM-Protein A beads TAB8848N3172-5MG 4°C 5 mg 175.00 Buy
HM-Protein A beads TAB8848N3172-20MG 4°C 20 mg 480.00 Buy
HM-Protein A beads TAB8848N3172-100MG 4°C 100 mg 1,920.00 Buy
HM-Protein G beads TAB8848N3173-5MG 4°C 5 mg 175.00 Buy
HM-Protein G beads TAB8848N3173-20MG 4°C 20 mg 480.00 Buy
HM-Protein G beads TAB8848N3173-100MG 4°C 100 mg 1,920.00 Buy