FG beads®: Line-up
Plain beads / Linker beads (Epoxy beads)
- Plain beads
For immobilization of DNA with a surface epoxy groups. Purification of a transcription factor and complementary DNA or RNA is one of applications.
| Storage buffer | ultrapure water |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | Epoxy groups |
| Amounts of the functional groups | Approx. 1μmol/mg |
>> For more information, please visit here.
- Linker beads (Epoxy beads)
It is difficult for the OH groups to immobilize it to beads in reactivity being low, but phenol groups can be immobilized on epoxy beads under an alkaline condition because reactivity is high. Because epoxy beads produce almost no non-specific protein adsorption, it is not necessary to mask the remaining epoxy groups.
| Storage buffer | ultrapure water |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | Epoxy groups |
| Amounts of the functional groups | 200-300 nmol/mg |
NH2 beads / OH beads
- NH2 beads
Compounds with COOH groups are immobilized on NH2 beads by covalent bond. But it is necessary to let a ligand activate with NHS ester.
| Storage buffer | ultrapure water |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | NH2 groups |
| Amounts of the functional groups | 200-300 nmol/mg |
>> For more information, please visit here.
- OH beads
When the reactivity of the COOH groups is high, compounds with COOH groups are immobilized on OH beads by covalent bond. Because OH beads produce almost no non-specific protein adsorption, it is not necessary to mask the remaining OH groups.
| Storage buffer | ultrapure water |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | OH groups |
| Amounts of the functional groups | 200-300 nmol/mg |
NHS beads / COOH beads
- NHS beads
Compounds with NH2 groups are immobilized on COOH beads by covalent bond easily. Therefore we can purify target protein at high purity from samples such as the cell extract. And, because antibodies or proteins with NH2 groups are immobilized easily, we can use it for immunoprecipitation and cell separation.
| Storage buffer | isopropyl alcohol |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | NHS groups |
| Amounts of the functional groups | Approx. 1μmol/mg |
>> For more information, please visit here.
- COOH beads
Compounds, antibodies and proteins with NH2 groups are immobilized on COOH beads by covalent bond. We can use it in wide application such as the chemical biology or cell separation.
| Storage buffer | ultrapure water |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | COOH groups |
| Amounts of the functional groups | 200-300 nmol/mg |
Azide beads / Alkyne beads
- Azide beads
Compounds with alkyne are immobilized on azide beads easily by using a click chemistry reaction. When a compound has plural one functional group, we can immobilize the compound which we introduced an alkyne into on azide beads regiospecifically.
| Storage buffer | ultrapure water |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | Azide groups |
| Amounts of the functional groups | 70-140 nmol/mg |
>> For more information, please visit here.
- Alkyne beads
Compounds with azide groups are immobilized on alkyne beads easily by using a click chemistry reaction. When a compound has plural one functional group, we can immobilize the compound which we introduced an azide group into on alkyne beads regiospecifically.
| Storage buffer | ultrapure water |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | Alkyne groups |
| Amounts of the functional groups | 70-140 nmol/mg |
Ts beads
- Ts beads
His tagged proteins can be immobilized to Ts beads selectively. This shows superiority for elucidation of the protein-protein interaction.
| Storage buffer | 1mM HCl |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | Ts groups |
| Amounts of the functional groups | 200-300 nmol/mg |
Protein A beads / Protein G beads / Streptavidin beads / NeutrAvidin beads
- Protein A beads
Protein A beads are combined with antibody (IgG) mainly. We can use it for the immunoprecipitation experiment using an antigen - antibody reaction or the antibody purification.
| Storage buffer | 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10% glycerol |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | Protein A |
| Capacity | >35 ug Human IgG/mg of beads |
>> For more information, please visit here.
- Protein G beads
Protein G beads are combined with antibody (IgG) mainly. We can use it for the immunoprecipitation experiment using an antigen - antibody reaction or the antibody purification.
| Storage buffer | 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | Protein G |
| Capacity | >35 ug Human IgG/mg of beads |
>> For more information, please visit here.
- Streptavidin beads
Biotinylated compounds and biotin-labeled antibody or DNA can be immobolized on Streptavidin beads, and we can use it in wide application. In addition, we can use Streptavidin beads for the separation by biotin-labeled cell.
| Storage buffer | 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | Streptavidin |
| Capacity | >3 ug biotinylated BSA/mg of beads |
- NeutrAvidin beads
Biotinylated compounds and biotin-labeled antibody or DNA can be immobolized on NeutrAvidin beads, and we can use it in wide application.
| Storage buffer | 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol |
|---|---|
| Magnetization | superparamagnetism (>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | NeutrAvidin |
| Capacity | >1.5 ug biotinylated BSA/mg of beads |
Ordering Information
| Product | Cat.No. | Storage | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| Plain beads | TAS8848N1010-10MG | 4°C | 10 mg | 247.00 | Buy |
| Plain beads | TAS8848N1010-20MG | 4°C | 20 mg | 274.00 | Buy |
| Linker beads (Epoxy beads) | TAS8848N1110-5MG | 4°C | 5 mg | 164.00 | Buy |
| Linker beads (Epoxy beads) | TAS8848N1110-20MG | 4°C | 20 mg | 420.00 | Buy |
| OH beads | TAS8848N1120-5MG | 4°C | 5 mg | 240.00 | Buy |
| OH beads | TAS8848N1120-20MG | 4°C | 20 mg | 555.00 | Buy |
| NH2 beads | TAS8848N1130-5MG | 4°C | 5 mg | 240.00 | Buy |
| NH2 beads | TAS8848N1130-20MG | 4°C | 20 mg | 555.00 | Buy |
| COOH beads | TAS8848N1140-5MG | 4°C | 5 mg | 274.00 | Buy |
| COOH beads | TAS8848N1140-20MG | 4°C | 20 mg | 683.00 | Buy |
| NHS beads | TAS8848N1141-5MG | -20°C | 5 mg | 342.00 | Buy |
| NHS beads | TAS8848N1141-10MG | -20°C | 10 mg | 547.00 | Buy |
| NHS beads | TAS8848N1141-20MG | -20°C | 20 mg | 956.00 | Buy |
| Ts beads | TAS8848N1150-5MG | 4°C | 5 mg | 274.00 | Buy |
| Ts beads | TAS8848N1150-20MG | 4°C | 20 mg | 683.00 | Buy |
| Azide beads | TAS8848N1160-5MG | 4°C | 5 mg | 479.00 | Buy |
| Azide beads | TAS8848N1160-10MG | 4°C | 10 mg | 683.00 | Buy |
| Azide beads | TAS8848N1160-20MG | 4°C | 20 mg | 1,092.00 | Buy |
| Alkyne beads | TAS8848N1161-5MG | 4°C | 5 mg | 479.00 | Buy |
| Alkyne beads | TAS8848N1161-10MG | 4°C | 10 mg | 683.00 | Buy |
| Alkyne beads | TAS8848N1161-20MG | 4°C | 20 mg | 1,092.00 | Buy |
| HM-Streptavidin beads | TAB8848N3170-5MG | 4°C | 5 mg | 956.00 | Buy |
| HM-Streptavidin beads | TAB8848N3170-10MG | 4°C | 10 mg | 1,366.00 | Buy |
| HM-Streptavidin beads | TAB8848N3170-20MG | 4°C | 20 mg | 2,184.00 | Buy |
| HM-NeutrAvidin beads | TAB8848N3171-5MG | 4°C | 5 mg | 956.00 | Buy |
| HM-NeutrAvidin beads | TAB8848N3171-10MG | 4°C | 10 mg | 1,366.00 | Buy |
| HM-NeutrAvidin beads | TAB8848N3171-20MG | 4°C | 20 mg | 2,184.00 | Buy |
| HM-Protein A beads | TAB8848N3172-5MG | 4°C | 5 mg | 956.00 | Buy |
| HM-Protein A beads | TAB8848N3172-20MG | 4°C | 20 mg | 1,366.00 | Buy |
| HM-Protein A beads | TAB8848N3172-100MG | 4°C | 100 mg | 2,184.00 | Buy |
| HM-Protein G beads | TAB8848N3173-5MG | 4°C | 5 mg | 956.00 | Buy |
| HM-Protein G beads | TAB8848N3173-20MG | 4°C | 20 mg | 1,366.00 | Buy |
| HM-Protein G beads | TAB8848N3173-100MG | 4°C | 100 mg | 2,184.00 | Buy |
| Streptavidin beads | TAS8848N1170-5MG | 4°C | 5mg | 479.00 | Buy |
| Streptavidin beads | TAS8848N1170-10MG | 4°C | 10mg | 683.00 | Buy |
| Streptavidin beads | TAS8848N1170-20MG | 4°C | 20mg | 1,092.00 | Buy |
| NeutrAvidin beads | TAS8848N1171-5MG | 4°C | 5mg | 479.00 | Buy |
| NeutrAvidin beads | TAS8848N1171-10MG | 4°C | 10mg | 683.00 | Buy |
| NeutrAvidin beads | TAS8848N1171-20MG | 4°C | 20mg | 1,092.00 | Buy |
| Protein A beads | TAS8848N1172-5MG | 4°C | 5mg | 479.00 | Buy |
| Protein A beads | TAS8848N1172-10MG | 4°C | 10mg | 683.00 | Buy |
| Protein A beads | TAS8848N1172-20MG | 4°C | 20mg | 1,092.00 | Buy |
| Protein G beads | TAS8848N1173-5MG | 4°C | 5mg | 479.00 | Buy |
| Protein G beads | TAS8848N1173-10MG | 4°C | 10mg | 683.00 | Buy |
| Protein G beads | TAS8848N1173-20MG | 4°C | 20mg | 1,092.00 | Buy |
Storage buffer of HM beads: 10 mM HEPES (pH 7.9)