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Recombinant Human CDK2/Cyclin E1 Complex

Cyclin-dependent kinase 2, p33

CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1/S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.


Cyclin-dependent kinase 2, 933
Molecular Weight : 
91 kDa (complex), 60.2 kDa (CDK2), 73.4 kDa (Cyclin E1)
Tag : 
N-GST (CDK2), N-6xHis (Cyclin E1)
Source :
Sf9 insect cells
Purity :
Greater than 95%, as determined by SDS-PAGE
Activity : 
Concentration : 0.5 mg/ml
Formulation : 
50 mM TrisCl pH 7.5, 150 mM NaCl, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 10 mM Glutathione, 25% Glycerol 
GeneBank Accession # :
NM_001798.5, a.a. 1-298 (CDK2)
NM_001238.4, a.a. 1-410 (Cyclin E1)
Application :  Inhibitor Screening, Kinding Assays
Storage :  Stable for 12 months at -80°C, Avoid freeze/thaw cycles
Reference : 
Levkau, B. et al: Cleavage of p21(Cip1/Waf1) and p27(Kip1) mediates apoptosis in endothelial cells through activation of Cdk2: role of a caspase cascade. Molec. Cell1: 553‐563, 1998.
Huang, H. et al: CDK2‐dependent phosphorylation of FOXO1 as an apoptotic response to DNA damage. Science314: 294‐297, 2006.
Ohtsubo, M., et al., Mol Cell Biol. 1995;15(5):2612-2624.
Harbour, J.W., et al., Cell. 1999;98(6):859-869.

Ordering Information

Product Purity Storage Cat.No. PKG Size Price  
Human CDK2/CyclinE1 95 % or more (SDS-PAGE) -80°C NU03041-1 10 µg 277.00 Buy
Human CDK2/CyclinE1 95 % or more (SDS-PAGE) -80°C NU03041-2 100 µg 1,279.00 Buy