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Recombinant Human ME1, ME2, ME3 (Malic Enzymes)

Malic Enzymes catalyze the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as cofactors. These proteins have a wide variety of uses including but not limited to; Protein binding Assays, Small Molecule Inhibitor Screens,Western blotting, ELISA, Crystallization and Co-Crystallization studies, Kinase substrates, and human antigen studies.

 

Recombinant Human ME1

ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

 

Synonym(s): 
NADP-dependent malic enzyme, Malic enzyme 1
Molecular Weight : 
65.2 kDa
Tag : 
His tag C-terminus
Source :
E. coli
Purity :
Greater than 95%, as determined by SDS-PAGE
Concentration :
0.5 mg/ml
Formulation : 
30 mM Tris pH 7.4; 500 mM NaCl; 10% glycerol; 3 mM MgCl2; 5 mM beta-mercaptoethanol; 1 mM PMSF; 1 mM Benzamidine
Reconstitution : 
Clear solution in 30 mM Tris, pH7.4, 150 mM NaCl, 40% Glycerol, 5 mM DTT
GeneBank Accession # :
NM_001798.5, a.a. 1-298 (CDK2)
NM_001237.5, a.a. 1-432 (Cyclin A2)
Application : 
Inhibitor Screening, Kinase Assays
Storage : 
Upon delivery aliquot and store at -80 °C. Avoid multiple freeze-thaw cycles. The protein is stable for 12 months at -80 °C, for 2-4 weeks at 4°C.
Suggested Usage : 
Protein binding assay, small molecule inhibitor screening, substrate of kinase, antigen, ELISA, Western blot, crystallization and co- crystallization studies.

Ordering Information

Product Cat.No. Purity Storage PKG Size Price(US$)  
Recombinant Human ME1 NU03052-1 95 % or more (SDS-PAGE) -80°C 10 µg 199.00 Buy
Recombinant Human ME1 NU03052-2 95 % or more (SDS-PAGE) -80°C 25 µg 279.00 Buy
Recombinant Human ME1 NU03052-3 95 % or more (SDS-PAGE) -80°C 100 µg 499.00 Buy
Recombinant Human ME1 NU03052-4 95 % or more (SDS-PAGE) -80°C 1 mg 1,599.00 Buy
Recombinant Human ME2 NU03053-2 95 % or more (SDS-PAGE) -80°C 10 µg 119.00 Buy
Recombinant Human ME2 NU03053-3 95 % or more (SDS-PAGE) -80°C 25 µg 135.00 Buy
Recombinant Human ME2 NU03053-4 95 % or more (SDS-PAGE) -80°C 100 µg 400.00 Buy
Recombinant Human ME2 NU03053-6 95 % or more (SDS-PAGE) -80°C 1 mg 1,399.00 Buy
Recombinant Human ME3 NU03054-1 95 % or more (SDS-PAGE) -80°C 10 µg 149.00 Buy
Recombinant Human ME3 NU03054-2 95 % or more (SDS-PAGE) -80°C 50 µg 249.00 Buy
Recombinant Human ME3 NU03054-3 95 % or more (SDS-PAGE) -80°C 100 µg 399.00 Buy
Recombinant Human ME3 NU03054-4 95 % or more (SDS-PAGE) -80°C 500 µg 699.00 Buy
Recombinant Human ME3 NU03054-5 95 % or more (SDS-PAGE) -80°C 1 mg 1,599.00 Buy