3D Nerve on a Chip / Starting the Neurorevolution
Jiksak Bioengineering develops research tools and treatments for neurological diseases, with a focus on peripheral nerve disorders. Jiksak means ”axon” in Japanese and our technology revolves around the axon.
Nerve Organoids™ consist of iPS-derived nerves in a microfluidics device. The nerves formed are very similar to in vivo nerves, with long axons extending from the cell body. Nerve Organoids are used for drug screening and mechanistic studies to discover genes underlying neurological diseases. See our papers for research applications of this technology!
A-Chip conists sof 2 wells connected by a microchannel. iPSC-derived neuronal spheres are seeded into the smaller well and axons grow into the microchannel. After culturing for several weeks, the axons will self-organize into a bundle and extend into the far well. Cell body and axon bundle can be isolated separately for protein analysis, RNA extraction, and other molecular analyses.
B-Chip consists of 2 wells connected by a microchannel. The 2 wells are separated by a wall, allowing researchers to culture cells in different media in each well. iPSC-derived neuronal spheres are seeded into the smaller well and axons grow into the microchannel. During culture, axons self-organize into a bundle and extend into the far well where they branch out and expand uniformly into growth cones. B-Chips can be used to co-culture target cell types and to induce human synapse formation using our synaptogenic beads. B-chip fits 48 chips into an SBS standard 96-well plate, making it compatible with imaging systems.
Kawada, J., Kaneda, S., Kirihara, T., Maroof, A., Levi, T., Eggan, K., Fujii, T. and Ikeuchi, Y., 2017. Generation of a motor nerve organoid with human stem cell-derived neurons. Stem cell reports, 9(5), pp.1441-1449.
Akiyama, T., Suzuki, N., Ishikawa, M., Fujimori, K., Sone, T., Kawada, J., Funayama, R., Fujishima, F., Mitsuzawa, S., Ikeda, K. and Ono, H., 2019. Aberrant axon branching via Fos-B dysregulation in FUS-ALS motor neurons. EBioMedicine, 45, pp.362-378.
Osaki, T., Chow, S.Y.A., Nakanishi, Y., Hernández, J., Kawada, J., Fujii, T. and Ikeuchi, Y., 2020. Three-dimensional motor nerve organoid generation. JoVE (Journal of Visualized Experiments), (163), p.e61544.
Mitsuzawa, S., Suzuki, N., Akiyama, T., Ishikawa, M., Sone, T., Kawada, J., Funayama, R., Shirota, M., Mitsuhashi, H., Morimoto, S. and Ikeda, K., 2021. Reduced PHOX2B stability causes axonal growth impairment in motor neurons with TARDBP mutations. Stem cell reports, 16(6), pp.1527-1541.
Video shot at 10X magnification and has been sped up by approximately 17x
|Nerve Organoid™ A-chip (cop/cop)||JKSK_S0001A_1||Room Temp.||1 chip||110.00||Buy|
|Nerve Organoid™ A-chip (cop/cop)||JKSK_S0001A_8||Room Temp.||8 chips/plate||875.00||Buy|
|Nerve Organoid™ A-chip (PDMS/Glass)||JKSK_S0002A_1||Room Temp.||1 chip||110.00||Buy|
|Nerve Organoid™ A-chip (PDMS/Glass)||JKSK_S0002A_8||Room Temp.||8 chips/plate||875.00||Buy|
|Nerve Organoid™ B-chip||JKSK_S0001B_1||Room Temp.||1 chip||110.00||Buy|
|Nerve Organoid™ B-chip||JKSK_S0001B_48||Room Temp.||48 chips/plate||5,251.00||Buy|
|A-chip Plate Holder||JKSK_S0001AP||Room Temp.||1 unit||19.00||Buy|
|A-chip Dummy Chip||JKSK_S0001AD_1||Room Temp.||1 unit||12.00||Buy|
|A-chip Dummy Chip||JKSK_S0001AD_5||Room Temp.||5 units||41.00||Buy|
|B-chip Plate Holder||JKSK_S0001BP||Room Temp.||1 unit||19.00||Buy|
|B-chip Dummy Chip||JKSK_S0001BD_1||Room Temp.||1 unit||16.00||Buy|
|B-chip Dummy Chip||JKSK_S0001BD_5||Room Temp.||5 units||59.00||Buy|
These products are manufactured by Jiksak Bioengineering, Inc.