Nacalai USA - Innovations for Life Sciences

Subtotal: $0.00

View Cart

Blocking Reagent for Immunoassays: Blocking One

Blocking Reagents / for WB and ELISA

Blocking is indispensable in immunoassays in order to block non-specific binding reactions. Blocking One contains both high molecular weight compounds and bovine serum protein. Blocking One is superior to conventional blocking solutions. The preservative in Blocking One does not affect the enzyme activity of peroxidase (POD) or alkaline phosphatase (ALP). Only simple refrigerator storage is necessary, even after opening the bottle.

Fast: in many assays a reduction of incubation time for blocking can be realized

Ready-to-use solution: simple storage in refrigerator even after opening the bottle

Comparison of blocking efficiency

The relationship between the reaction time and the blocking efficiency in microplate assay. In comparison with other products, Blocking One shows the fastest dynamics.

Detection of Mouse IgG (H+L) by Western Blotting

Incubation time for blocking is 20 mins.

M:  Biotinylated Protein Marker
(1): Mouse Serum 2.5 ul
(2): Mouse Serum 5 ul
(3): Mouse Serum 10 ul

Mouse IgG (H+L) in Mouse Serum
detected with Biotinylated anti Mouse IgG (H+L)

Very little background occurs, indicating exceptional blocking performance.


  1. Masuda, Takahito, et al. "Anti-HER2 antibody therapy using gene-transduced adipocytes for HER2-positive breast cancer." Breast Cancer Research and Treatment (2020): 1-10.
  2. Niwa, Toshio, et al. "Equol Inhibits Mushroom Tyrosinase in Vitro through Tight Binding." Biological and Pharmaceutical Bulletin 43.3 (2020): 550-553.
  3. Kamata, Kazuma, et al. "Generation of a human induced pluripotent stem cell line, BRCi004-A, derived from a patient with age-related macular degeneration." Stem Cell Research (2020): 101787.
  4. Sakushima, Koki, et al. "Moderate hypoxia promotes skeletal muscle cell growth and hypertrophy in C2C12 cells." Biochemical and Biophysical Research Communications (2020).
  5. Shirai, Takanaga, et al. "The order of concurrent training affects mTOR signaling but not mitochondrial biogenesis in mouse skeletal muscle." Physiological Reports 8.7 (2020).
  6. Seita, Yasunari, et al. "Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein." Journal of Alzheimer's Disease Preprint (2020): 1-16.
  7. Tanaka, Hiroaki, et al. "Naquotinib exerts antitumor activity in activated B-cell-like diffuse large B-cell lymphoma." Leukemia Research 88 (2020): 106286.
  8. Kamata, Kazuma, et al. "Generation of a human induced pluripotent stem cell line, BRCi005-A, derived from a Best disease patient with BEST1 mutations." Stem Cell Research (2020): 101782.
  9. Hamada, Yoshimasa, et al. "Nanosecond pulsed electric fields induce the integrated stress response via reactive oxygen species-mediated heme-regulated inhibitor (HRI) activation." PloS one 15.3 (2020): e0229948.
  10. Toda, Kazuya, et al. "Heat-Killed Bifidobacterium breve B-3 Enhances Muscle Functions: Possible Involvement of Increases in Muscle Mass and Mitochondrial Biogenesis." Nutrients 12.1 (2020): 219.
  11. Tanaka, Hiroyoshi Y., et al. "Heterotypic 3D pancreatic cancer model with tunable proportion of fibrotic elements." Biomaterials (2020): 120077.
  12. Takahashi, Masashi, et al. "Role of FOXO3A in Trastuzumab Combination Chemotherapy in Esophageal Squamous Cell Carcinoma." Anticancer Research 40.4 (2020): 1921-1930.
  13. Ogura, Yuji, et al. "Age-related decrease in muscle satellite cells is accompanied with diminished expression of early growth response 3 in mice." Molecular biology reports 47.2 (2020): 977-986.
  14. Murai, Yasutaka, et al. "Analysis of haemodynamics and angiogenic response to ischaemia in the obese type 2 diabetic model Spontaneously Diabetic Torii Leprfa (SDT fatty) rats." Clinical and Experimental Pharmacology and Physiology 47.4 (2020): 583-590.
  15. Kohno, Kakeru, et al. "Tocotrienol-rich fraction from annatto ameliorates expression of lysyl oxidase in human osteoblastic MG-63 cells." Bioscience, Biotechnology, and Biochemistry 84.3 (2020): 526-535.
  16. Tachibana, Mayumi, et al. "The C-terminal region of the Plasmodium yoelii microgamete surface antigen PyMiGS induces potent anti-malarial transmission-blocking immunity in mice." Vaccine (2020).
  17. Shinohara, Saki, and Ko Fujimori. "Promotion of lipogenesis by PPARγ-activated FXR expression in adipocytes." Biochemical and Biophysical Research Communications 527.1 (2020): 49-55.
  18. Umezu, Kohei, et al. "Stromal cell-derived factor 1 regulates in vitro sperm migration towards the cumulus-oocyte complex in cattle." PloS one 15.4 (2020): e0232536.
  19. Tokinoya, Katsuyuki, et al. "Moderate‐intensity exercise increases renalase levels in the blood and skeletal muscle of rats." FEBS Open Bio (2020).
  20. Nishida-Aoki, Nao, et al. "Altered biodistribution of deglycosylated extracellular vesicles through enhanced cellular uptake." Journal of Extracellular Vesicles 9.1 (2020): 1713527.
  21. Dr. Takanori Takebe's group: Koido, Masaru et al. "Polygenic architecture informs potential vulnerability to drug-induced liver injury." Nat Med. (2020): Online ahead of print. «« New!

Ordering Information

Product Storage Cat.No. PKG Size Price  
Blocking One 2-8°C 03953-66 100 ml 42.00 Buy
Blocking One 2-8°C 03953-95 500 ml 63.00 Buy