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COSMOSIL PFP

Pentafluorophenyl-bonded stationary phase

  • Pentafluorophenyl-bonded stationary phase
  • Alternative selectivity to C18 columns
  • Available in analytical and preparative columns
  • Suitable for structural isomers and halogenated compounds

COSMOSIL 5PFP
USP code L43
Silica gel High purity porous spherical silica
Average particle size 5 µm
Average pore size approx. 120Å
Specific surface area approx. 300 m²/g
Stationary phase Pentafluorophenyl group
Bonding type Monomeric
Endcapping treatment Yes
Carbon content approx. 10%
Usable pH range 2 - 7.5

Alternative Selectivity to C18 Columns

COSMOSIL PFP provides different selectivity from C18 Columns. Furthermore it offers improved separation compared to other companies' PFP columns.

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Methanol : Water = 40 : 60
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 254 nm
Sample: 1. o-Cresol (3.0 mg/ml)
2. m-Cresol (3.0 mg/ml)
3. p-Cresol (3.0 mg/ml)
Injection vol.: 1.0 µl
 

Separation Properties of PFP

COSMOSIL PFP uses dipole-dipole and π-πinteractions. It offers strong retention for compounds with strong dipole moments, e.g. cationic or halogenated compounds. It also has high steric selectivity for structural isomer separations.

 

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Methanol : Water = 50 : 50
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 254 nm
Sample: o-Dinitrobenzene
m-Dinitrobenzene
p-Dinitrobenzene
Benzene (I.S.)

Reasonably Priced Preparative Columns

We can offer preparative columns at a reasonable price because we synthesize our own silylating agents.

Column size 4.6 mmI.D. x 250 mm 10 mmI.D. x 250 mm 20 mmI.D. x 250 mm
HPLC system Analytical HPLC system Analytical HPLC system Semi-preparative HPLC system
Flow rate 1.0 ml/min 5.0 ml/min 19.0 ml/min
Injection vol. 10µl 50µl 190µl
Chromatogram
Mobile phase: Methanol : Water = 70 : 30
Temperature: 30°C
Detection: UV 254 nm
Sample: 1. Uracil
2. Methyl Benzoate
3. Toluene
4. Naphthalene

Applications

Fluorinated compounds

 

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Methanol : Water = 60 : 40
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 254 nm
Sample: 1. Benzene (2.5 mg/ml)
2. Fluorobenzene (1.0 mg/ml)
3. Toluene (2.5 mg/ml)
4. α, α, α-Trifluorotoluen
(Benzotrifluoride) (0.25 mg/ml)
Injection vol.: 1.0 µl

Vitamin E

Column size: 4.6 mmI.D. x 250 mm
Mobile phase: Methanol : Water = 90 : 10
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 295 nm
Sample: 1. δ-Tocotrienol
2. β-Tocotrienol
3. γ-Tocotrienol
4. α-Tocotrienol
5. δ-Tocopherol
6. β-Tocopherol
7. γ-Tocopherol
8. α-Tocopherol

  

Comparison of ion-pairing reagents

 

PFP × Fluorine ion-pairing reagent

Usage Information

Column5 µm PFP
I.D. (mm)2.03.04.610.020.028.0
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.
2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range pH2~pH7.5
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60℃. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.5 µm or finer filter before use.
- Acetonitrile is not recommended as a mobile phase.

Downloads

Brochures

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size

Ordering Information

Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL PFP Packed Column (2.0 mm I.D. x 50 mm) 13263-41 Waters type 1 pkg 400.00 Buy
COSMOSIL PFP Packed Column (2.0 mm I.D. x 100 mm) 13264-31 Waters type 1 pkg 500.00 Buy
COSMOSIL PFP Packed Column (2.0 mm I.D. x 150 mm) 12381-21 Waters type 1 pkg 580.00 Buy
COSMOSIL PFP Packed Column (2.0 mm I.D. x 250 mm) 13265-21 Waters type 1 pkg 670.00 Buy
COSMOSIL PFP Packed Column (3.0 mm I.D. x 50 mm) 13266-11 Waters type 1 pkg 430.00 Buy
COSMOSIL PFP Packed Column (3.0 mm I.D. x 100 mm) 13267-01 Waters type 1 pkg 500.00 Buy
COSMOSIL PFP Packed Column (3.0 mm I.D. x 150 mm) 13268-91 Waters type 1 pkg 480.00 Buy
COSMOSIL PFP Packed Column (3.0 mm I.D. x 250 mm) 13269-81 Waters type 1 pkg 630.00 Buy
COSMOSIL PFP Packed Column (4.6 mm I.D. x 50 mm) 13270-41 Waters type 1 pkg 430.00 Buy
COSMOSIL PFP Packed Column (4.6 mm I.D. x 100 mm) 13271-31 Waters type 1 pkg 500.00 Buy
COSMOSIL PFP Packed Column (4.6 mm I.D. x 150 mm) 12383-01 Waters type 1 pkg 505.00 Buy
COSMOSIL PFP Packed Column (4.6 mm I.D. x 250 mm) 12384-91 Waters type 1 pkg 630.00 Buy
COSMOSIL PFP Packed Column (10.0 mm I.D. x 50 mm) 13272-21 Waters type 1 pkg 800.00 Buy
COSMOSIL PFP Packed Column (10.0 mm I.D. x 100 mm) 13273-11 Waters type 1 pkg 1,030.00 Buy
COSMOSIL PFP Packed Column (10.0 mm I.D. x 150 mm) 13274-01 Waters type 1 pkg 1,120.00 Buy
COSMOSIL PFP Packed Column (10.0 mm I.D. x 250 mm) 12386-71 Waters type 1 pkg 1,440.00 Buy
COSMOSIL PFP Packed Column (20.0 mm I.D. x 50 mm) 13276-81 Waters type 1 pkg 1,380.00 Buy
COSMOSIL PFP Packed Column (20.0 mm I.D. x 100 mm) 13277-71 Waters type 1 pkg 2,400.00 Buy
COSMOSIL PFP Packed Column (20.0 mm I.D. x 150 mm) 13278-61 Waters type 1 pkg 2,520.00 Buy
COSMOSIL PFP Packed Column (20.0 mm I.D. x 250 mm) 12387-61 Waters type 1 pkg 3,350.00 Buy
COSMOSIL PFP Packed Column (28.0 mm I.D. x 100 mm) 13280-11 Waters type 1 pkg 5,100.00 Buy
COSMOSIL PFP Packed Column (28.0 mm I.D. x 150 mm) 13281-01 Waters type 1 pkg 5,400.00 Buy
COSMOSIL PFP Packed Column (28.0 mm I.D. x 250 mm) 13282-91 Waters type 1 pkg 7,200.00 Buy
Guard Column / Guard Cartridge
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL PFP Guard Cartridge (4.6 mm I.D. x 10 mm) 12443-24 Waters type 2 pkg 210.00 Buy
COSMOSIL PFP Guard Column (10.0 mm I.D. x 20 mm) 12385-81 Waters type 1 pkg 505.00 Buy
COSMOSIL PFP Guard Column (20.0 mm I.D. x 20 mm) 13275-91 Waters type 1 pkg 1,200.00 Buy
COSMOSIL PFP Guard Column (28.0 mm I.D. x 20 mm) 13279-51 Waters type 1 pkg 4,200.00 Buy
HPLC Accessories
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL Direct Cartridge Holder 19989-71 Waters type 1 pkg 270.00 Buy
COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) 38009-79 Waters type 1 pkg 265.00 Buy
COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) 11884-71 Waters type 1 pkg 265.00 Buy
COSMOSIL Column Prefilter 39361-19 Waters type 1 pkg 145.00 Buy
COSMOSIL Column Spare Filter for Prefilter (1 micron) 39539-09 Waters type 2 pkg 55.00 Buy
COSMOSIL Column Connecting Tube (0.25 mm) 37843-69 Waters type 1 pkg 65.00 Buy
COSMOSIL Column Connecting Tube (0.1 mm) 12570-41 Waters type 1 pkg 65.00 Buy