COSMOSIL Cholester
Specialty for Structual Isomers / Hydrophobic interaction and planarity selectivity
COSMOSIL Cholester is a new silica based RP column which employs a cholestery bonded phase. Cholester provides enhanced selectivity over traditional C18 materials and greater performance in separating isomers or other closely related compounds. Cholester is ideal for method development and serves as an excellent alternative to traditional C18 columns.
| COSMOSIL Cholester | |||
|---|---|---|---|
| USP code | L101 | ||
| Silica gel | High purity porous spherical silica | ||
| Average particle size | 2.5µm | 3µm | 5µm |
| Average pore size | approx. 130Å | approx. 120Å | approx. 120Å |
| Specific surface area | approx. 330m2/g | approx. 300m2/g | approx. 300m2/g |
| Stationary phase | Cholesteryl group | ||
| Bonding type | Monomeric | ||
| End capping treatment | Near-perfect treatment | ||
| Carbon content | approx. 21% | - | approx. 20% |
| pH range | 2 - 7.5 | ||
Hydrophobic Interaction
Separation of Alkylbenzene (comparison with hydrophobicity)
Figure shows the comparison of hydrophobic interaction with competitive C18 and C30 columns. Cholester provides the same hydrphobicity as alkyl group bonded type (C18. C30). It is not necessary to change the analytical conditions when replacing C18 or C30 columns with Cholester.
Conditions:
| Column Size | 4.6 mmI.D. x 150 mm | ||
| Mobile Phase | Methanol : Water = 80 : 20 | ||
| Flow Rate | 1.0 ml/min | ||
| Temperature | 30 oC | ||
| Detection | UV 254 nm | ||
| Sample |
|
Planarity Selectivity
The stationary phase of Cholester has very rigit structure and can distinguish different molecular shapes. Cholester offers improved separation for structurally similar compunds that are difficult to analyze with alkyl group bonded materials (C18 and C30). As in the following example Cholester retains planar Triphenylene longer than stereoscopic o-Therphenyl.
Planarity Selectivity (Planarity : o-Terphenyl < Triphenylene)
Cholester shows greater planarity selectivity.
Conditions:
| Column Size | 4.6 mmI.D. x 150 mm | ||
| Mobile Phase | 90% Methanol | ||
| Flow Rate | 1.0 ml/min | ||
| Temperature | 30 oC | ||
| Detection | UV 254 nm | ||
| Sample |
|
Applications
Characteristics of separation
Aryl Halides (Relative strengths of nucleophiles : Methyl < Chlorine < Bromine)
Cholester is more selective for Aryl Halides.
Conditions:
| Column Size | 4.6 mmI.D. x 150 mm | ||
| Mobile Phase | 60% Methanol | ||
| Flow Rate | 1.0 ml/min | ||
| Temperature | 30 oC | ||
| Detection | UV 254 nm | ||
| Sample |
|
Steroids like D Vitamins
Cholesteryl bonded groups are very rigid structures and offer improved separation of compounds that are difficult to analyze with alkyl group bonded materials.
Conditions:
| Column Size | 4.6 mmI.D. x 150 mm | ||
| Mobile Phase | 100% Methanol | ||
| Flow Rate | 1.0 ml/min | ||
| Temperature | 30 oC | ||
| Detection | UV 265 nm | ||
| Sample |
|
Diasteromers like Stilbenes
Cholester offers improved resolution of geometric isomers.
Conditions:
| Column Size | 4.6 mmI.D. x 150 mm | ||
| Mobile Phase | 70% Methanol | ||
| Flow Rate | 1.0 ml/min | ||
| Temperature | 30 oC | ||
| Detection | UV 254 nm | ||
| Sample |
|
Position isomers like Chloroacetophenones and Terphenyls
Generally isomers of disubstituted benzene are difficult to separate with C18 because their structual and polar characteristics are veryb similar. In these cases COSMOSIL Cholester works very well.
Conditions:
| Column Size | 4.6 mmI.D. x 150 mm | ||
| Mobile Phase | 50% Methanol | ||
| Flow Rate | 1.0 ml/min | ||
| Temperature | 30 oC | ||
| Detection | UV 254 nm | ||
| Sample |
|
Catechines
Conditions:
| Column Size | 4.6 mmI.D. x 150 mm | ||
| Mobile Phase |
A: Acetonitrile : 20mmol/l Phosphate Buffer (pH2.5) = 10 : 90 |
||
| Flow Rate | 1.0 ml/min | ||
| Temperature | 30 oC | ||
| Detection | UV 284 nm | ||
| Sample |
|
||
| Injection Vol. | 1 µl |
Applications -2
NMUR2 selective agonist
Takayama, Kentaro, et al. "A chemically stable peptide agonist to neuromedin U receptor type 2." Bioorganic & medicinal chemistry 28.10 (2020): 115454.
D-DLDA-DL-Amino Acids
Application data for isomers of 13-hydroxy-octadecadienoic acid
These compounds have been found to be related to several diseases, including atherosclerosis, asthma, and several types of cancer. As the risk of disease is related to the ratio of the cis/trans isomers, the ability to separate them chromatographically is important.
One of our customers has developed a method to separate two of these isomers using our COSMOSIL Cholester column. This column excels at separation of geometric isomers.
Usage Information
| Column | 5 µm Cholester | |||||
|---|---|---|---|---|---|---|
| I.D. (mm) | 2.0 | 3.0 | 4.6 | 10.0 | 20.0 | 28.0 |
| Washing method | 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. 2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF. |
|||||
| Storage conditions | Short-term (up to a week): Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. Long-term: Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water. In either case, tightly plug the column, and store in a cool and dry place. |
|||||
| Usable pH range | pH2～pH7.5 | |||||
| Max. pressure | 20MPa | 15MPa | ||||
| Temperature range | The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C. | |||||
| Usable solvents | Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable. Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points. |
|||||
| Other instructions | - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. - Always filter mobile phases using a 0.5 µm or finer filter before use. |
|||||
Downloads
Brochures
- COSMOSIL Cholester
(640 KB) - COSMOSIL 2.5Cholester (1.2 MB)
- Analysis of Natural Compounds by 2.5Cholester (390 KB)
- Analysis of New Antimycotic Substances (5aTHQs) by COSMOSIL Cholester (1 MB)
- COSMOSIL/COSMOCORE Cholester Series Application Notebook and Reference List (14 MB)
Posters
- Identifying Vitamin D2 and D3 and their 25-OH Metabolites and C3 Epimers in a Single LC-MS Run (Poster from Food Science 2016, Singapore) (0.2 MB)
- Simultaneous Separation of Fat-Soluble Vitamins by Reversed-Phase HPLC Using a Cholesteryl Group Bonded Stationary Phase (Poster from AOAC Annual Meeting & Exposition 2013, Chicago, IL USA) (0.2 MB)
- Rapid HPLC Method Development with C18 and Phenyl Columns
(Poster from HPLC2013, Amsterdam, the Netherlands) (0.2 MB) - Simultaneous Separation of Fat-Soluble Vitamins by Reversed-Phase HPLC Using a Cholesteryl Group Bonded Stationary Phase
(Poster from AOAC Annual Meeting 2012, Las Vegas, Nevada USA) (0.2 MB)
COSMOSIL Application Search
COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size.
Reference
- Jomori, Takahiro, et al. "Mycobacterium smegmatis alters the production of secondary metabolites by marine-derived Aspergillus niger." Journal of Natural Medicines 74.1 (2020): 76-82.
- Sibero, Mada Triandala, et al. "Chromanone-type compounds from marine sponge-derived Daldinia eschscholtzii KJMT FP 4.1." Journal of Applied Pharmaceutical Science 10.1 (2020): 001-007.
- ZAIMA, Kazumasa, et al. "Application of High-Speed Countercurrent Chromatography to the Separation of Flavanonol, Phenylcoumaran and Flavonolignans in Silybum marianum." CHROMATOGRAPHY (2020).
- Sato, Tomoyuki, et al. "Cadinane sesquiterpenoids isolated from Santalum album using a screening program for Wnt signal inhibitory activity." Journal of Natural Medicines 74.2 (2020): 476-481.
- Takayama, Kentaro, et al. "A chemically stable peptide agonist to neuromedin U receptor type 2." Bioorganic & Medicinal Chemistry (2020): 115454.
- Janicka, Małgorzata, Małgorzata Sztanke, and Krzysztof Sztanke. "Predicting the Blood-Brain Barrier Permeability of New Drug-Like Compounds via HPLC with Various Stationary Phases." Molecules 25.3 (2020): 487.
- Nakayama, Hideyuki, et al. "Glucose esters of caffeic acid from Allium macrostemon Bunge." Japanese Journal of Food Chemistry and Safety 27.1 (2020): 28-32.
- Ono, Masateru, et al. "Identification and characterization of organic and glycosidic acids in crude resin glycoside fraction from Calystegia hederacea." Journal of Natural Medicines 74.1 (2020): 200-211.
- Kaunda, Joseph Sakah, and Ying-Jun Zhang. "Two new 23S, 26R-hydroxylated spirostanoid saponins from the fruits of Solanum indicum var. recurvatum." Steroids 153 (2020): 108506.
- Katsutani, Kazuma, et al. "Eudesmane-type sesquiterpene glycosides: sonneratiosides A–E and eudesmol β-d-glucopyranoside from the leaves of Sonneratia alba." Journal of Natural Medicines (2020): 1-8.
- Dougnon, Godfried, and Michiho Ito. "Medicinal uses, thin-layer chromatography and high-performance liquid chromatography profiles of plant species from Abomey-Calavi and Dantokpa Market in the Republic of Benin." Journal of Natural Medicines 74.1 (2020): 311-322.
COSMOSIL Application Search
COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size
Ordering Information
2.5 µm particle size
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 2.5Cholester Packed Column (2.0 mmI.D. x 50 mm) | End-Fitting: Waters type | 09000-01 | 1 pkg | 800.00 | Buy |
| COSMOSIL 2.5Cholester Packed Column (2.0 mmI.D. x 75 mm) | End-Fitting: Waters type | 09047-11 | 1 pkg | 800.00 | Buy |
| COSMOSIL 2.5Cholester Packed Column (2.0 mmI.D. x 100 mm) | End-Fitting: Waters type | 09048-01 | 1 pkg | 800.00 | Buy |
| COSMOSIL 2.5Cholester Packed Column (3.0 mmI.D. x 50 mm) | End-Fitting: Waters type | 09049-91 | 1 pkg | 800.00 | Buy |
| COSMOSIL 2.5Cholester Packed Column (3.0 mmI.D. x 75 mm) | End-Fitting: Waters type | 09050-51 | 1 pkg | 800.00 | Buy |
| COSMOSIL 2.5Cholester Packed Column (3.0 mmI.D. x 100 mm) | End-Fitting: Waters type | 09051-41 | 1 pkg | 800.00 | Buy |
| COSMOSIL 2.5Cholester Packed Column (3.0 mmI.D. x 150 mm) | End-Fitting: Waters type | 07099-01 | 1 pkg | 965.00 | Buy |
| COSMOSIL 2.5Cholester Packed Column (4.6 mmI.D. x 150 mm) | End-Fitting: Waters type | 13838-21 | 1 pkg | 965.00 | Buy |
3 µm particle size
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 3Cholester Packed Column (2.0 mmI.D. x 50 mm) | End-Fitting: Waters type | 19188-61 | 1 pkg | 730.00 | Buy |
| COSMOSIL 3Cholester Packed Column (2.0 mmI.D. x 75 mm) | End-Fitting: Waters type | 19189-51 | 1 pkg | 730.00 | Buy |
| COSMOSIL 3Cholester Packed Column (2.0 mmI.D. x 100 mm) | End-Fitting: Waters type | 19190-11 | 1 pkg | 730.00 | Buy |
| COSMOSIL 3Cholester Packed Column (2.0 mmI.D. x 150 mm) | End-Fitting: Waters type | 19191-01 | 1 pkg | 730.00 | Buy |
| COSMOSIL 3Cholester Packed Column (2.0 mmI.D. x 250 mm) | End-Fitting: Waters type | 19192-91 | 1 pkg | 871.00 | Buy |
| COSMOSIL 3Cholester Packed Column (3.0 mmI.D. x 50 mm) | End-Fitting: Waters type | 19194-71 | 1 pkg | 730.00 | Buy |
| COSMOSIL 3Cholester Packed Column (3.0 mmI.D. x 75 mm) | End-Fitting: Waters type | 19195-61 | 1 pkg | 730.00 | Buy |
| COSMOSIL 3Cholester Packed Column (3.0 mmI.D. x 100 mm) | End-Fitting: Waters type | 19196-51 | 1 pkg | 730.00 | Buy |
| COSMOSIL 3Cholester Packed Column (3.0 mmI.D. x 150 mm) | End-Fitting: Waters type | 19197-41 | 1 pkg | 730.00 | Buy |
| COSMOSIL 3Cholester Packed Column (3.0 mmI.D. x 250 mm) | End-Fitting: Waters type | 19198-31 | 1 pkg | 871.00 | Buy |
| COSMOSIL 3Cholester Packed Column (4.6 mmI.D. x 50 mm) | End-Fitting: Waters type | 19199-21 | 1 pkg | 730.00 | Buy |
| COSMOSIL 3Cholester Packed Column (4.6 mmI.D. x 75 mm) | End-Fitting: Waters type | 19200-71 | 1 pkg | 730.00 | Buy |
| COSMOSIL 3Cholester Packed Column (4.6 mmI.D. x 100 mm) | End-Fitting: Waters type | 19300-61 | 1 pkg | 695.00 | Buy |
| COSMOSIL 3Cholester Packed Column (4.6 mmI.D. x 150 mm) | End-Fitting: Waters type | 19151-21 | 1 pkg | 695.00 | Buy |
| COSMOSIL 3Cholester Packed Column (4.6 mmI.D. x 250 mm) | End-Fitting: Waters type | 19316-71 | 1 pkg | 812.00 | Buy |
5 µm particle size
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 5Cholester Packed Column (1.0 mm I.D. x 150 mm) | End-Fitting: Waters type | 05968-71 | 1 pkg | 700.00 | Buy |
| COSMOSIL 5Cholester Packed Column (1.0 mm I.D. x 250 mm) | End-Fitting: Waters type | 05969-61 | 1 pkg | 823.00 | Buy |
| COSMOSIL 5Cholester Packed Column (2.0 mm I.D. x 30 mm) | End-Fitting: Waters type | 08565-51 | 1 pkg | 559.00 | Buy |
| COSMOSIL 5Cholester Packed Column (2.0 mm I.D. x 50 mm) | End-Fitting: Waters type | 06352-91 | 1 pkg | 653.00 | Buy |
| COSMOSIL 5Cholester Packed Column (2.0 mm I.D. x 100 mm) | End-Fitting: Waters type | 06948-01 | 1 pkg | 595.00 | Buy |
| COSMOSIL 5Cholester Packed Column (2.0 mm I.D. x 150 mm) | End-Fitting: Waters type | 05971-11 | 1 pkg | 600.00 | Buy |
| COSMOSIL 5Cholester Packed Column (2.0 mm I.D. x 250 mm) | End-Fitting: Waters type | 05972-01 | 1 pkg | 724.00 | Buy |
| COSMOSIL 5Cholester Packed Column (3.0 mm I.D. x 100 mm) | End-Fitting: Waters type | 15892-41 | 1 pkg | 595.00 | Buy |
| COSMOSIL 5Cholester Packed Column (3.0 mm I.D. x 150 mm) | End-Fitting: Waters type | 05973-91 | 1 pkg | 559.00 | Buy |
| COSMOSIL 5Cholester Packed Column (3.0 mm I.D. x 250 mm) | End-Fitting: Waters type | 05974-81 | 1 pkg | 635.00 | Buy |
| COSMOSIL 5Cholester Packed Column (4.6 mm I.D. x 50 mm) | End-Fitting: Waters type | 06359-21 | 1 pkg | 630.00 | Buy |
| COSMOSIL 5Cholester Packed Column (4.6 mm I.D. x 100 mm) | End-Fitting: Waters type | 06591-61 | 1 pkg | 595.00 | Buy |
| COSMOSIL 5Cholester Packed Column (4.6 mm I.D. x 150 mm) | End-Fitting: Waters type | 05976-61 | 1 pkg | 559.00 | Buy |
| COSMOSIL 5Cholester Packed Column (4.6 mm I.D. x 150 mm) (3 Lots Set) | End-Fitting: Waters type | 07970-03 | 3 pkg | 1,672.00 | Buy |
| COSMOSIL 5Cholester Packed Column (4.6 mm I.D. x 250 mm) | End-Fitting: Waters type | 05977-51 | 1 pkg | 607.00 | Buy |
| COSMOSIL 5Cholester Packed Column (6.0 mm I.D. x 150 mm) | End-Fitting: Waters type | 06445-81 | 1 pkg | 765.00 | Buy |
| COSMOSIL 5Cholester Packed Column (10.0 mm I.D. x 50 mm) | End-Fitting: Waters type | 16590-61 | 1 pkg | 988.00 | Buy |
| COSMOSIL 5Cholester Packed Column (10.0 mm I.D. x 100 mm) | End-Fitting: Waters type | 16591-51 | 1 pkg | 1,118.00 | Buy |
| COSMOSIL 5Cholester Packed Column (10.0 mm I.D. x 150 mm) | End-Fitting: Waters type | 08011-91 | 1 pkg | 1,177.00 | Buy |
| COSMOSIL 5Cholester Packed Column (10.0 mm I.D. x 250 mm) | End-Fitting: Waters type | 05979-31 | 1 pkg | 1,494.00 | Buy |
| COSMOSIL 5Cholester Packed Column (20.0 mm I.D. x 100 mm) | End-Fitting: Waters type | 15995-01 | 1 pkg | 2,297.00 | Buy |
| COSMOSIL 5Cholester Packed Column (20.0 mm I.D. x 150 mm) | End-Fitting: Waters type | 06088-71 | 1 pkg | 2,417.00 | Buy |
| COSMOSIL 5Cholester Packed Column (20.0 mm I.D. x 250 mm) | End-Fitting: Waters type | 05982-71 | 1 pkg | 3,217.00 | Buy |
| COSMOSIL 5Cholester Packed Column (28.0 mm I.D. x 100 mm) | End-Fitting: Waters type | 16592-41 | 1 pkg | 6,297.00 | Buy |
| COSMOSIL 5Cholester Packed Column (28.0 mm I.D. x 150 mm) | End-Fitting: Waters type | 16593-31 | 1 pkg | 6,627.00 | Buy |
| COSMOSIL 5Cholester Packed Column (28.0 mm I.D. x 250 mm) | End-Fitting: Waters type | 05985-41 | 1 pkg | 6,939.00 | Buy |
Guard Column / Guard Cartridge
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 3Cholester Guard Cartridge (2.0 mmI.D. x 10 mm) | End-Fitting: Waters type | 19325-64 | 2 pkg | 259.00 | Buy |
| COSMOSIL 3Cholester Guard Cartridge (4.6 mmI.D. x 10 mm) | End-Fitting: Waters type | 19344-14 | 2 pkg | 259.00 | Buy |
| COSMOSIL 5Cholester Guard Column (3.0 mm I.D. x 10 mm) | End-Fitting: Waters type | 06175-21 | 1 pkg | - | Inquire |
| COSMOSIL 5Cholester Guard Cartridge (4.6 mmI.D. x 10 mm) | End-Fitting: Waters type | 19183-24 | 2 pkg | 259.00 | Buy |
| COSMOSIL 5Cholester Guard Column (10.0 mm I.D. x 20 mm) | End-Fitting: Waters type | 05978-41 | 1 pkg | 559.00 | Buy |
| COSMOSIL 5Cholester Guard Column (20.0 mm I.D. x 20 mm) | End-Fitting: Waters type | 05980-91 | 1 pkg | 1,236.00 | Buy |
| COSMOSIL 5Cholester Guard Column (20.0 mm I.D. x 50 mm) | End-Fitting: Waters type | 05981-81 | 1 pkg | 1,930.00 | Buy |
| COSMOSIL 5Cholester Guard Column (28.0 mm I.D. x 50 mm) | End-Fitting: Waters type | 05983-61 | 1 pkg | 4,066.00 | Buy |
HPLC Accessories
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL Column Spare Filter for Prefilter (1 micron) | End-Fitting: Waters type | 39539-09 | 2 pkg | 60.00 | Buy |
| COSMOSIL Column Connecting Tube (0.25 mm) | End-Fitting: Waters type | 37843-69 | 1 pkg | 71.00 | Buy |
| COSMOSIL Column Connecting Tube (0.1 mm) | End-Fitting: Waters type | 12570-41 | 1 pkg | 71.00 | Buy |
| COSMOSIL Column Prefilter | End-Fitting: Waters type | 39361-19 | 1 pkg | 159.00 | Buy |
| COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) | End-Fitting: Waters type | 11884-71 | 1 pkg | 291.00 | Buy |
| COSMOSIL Direct Cartridge Holder | End-Fitting: Waters type | 19989-71 | 1 pkg | 297.00 | Buy |
| COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) **Discontinued** | End-Fitting: Waters type | 38009-79 | 1 pkg | - | Inquire |
** Guard cartridges require a guard cartridge holder.
** #38009-79 has been discontinued. Alternative product is COSMOSIL Direct Cartridge Holder (#19989-71).