Conditions:  

Column Size	4.6 mmI.D. x 150 mm
Mobile Phase	Methanol : Water = 80 : 20
Flow Rate	1.0 ml/min
Temperature	30 oC
Detection	UV 254 nm
Sample	1. Benzen (1.67 µg) 2. Toluene (1.67 µg) 3. Ethylbenzene (1.67 µg) 4. Propylbenzene (1.67 µg) 5. Butylbenzene (1.67 µg) 6. Amylbenzene (1.67 µg)

The stationary phase of Cholester has very rigit structure and can distinguish different molecular shapes. Cholester offers improved separation for structurally similar compunds that are difficult to analyze with alkyl group bonded materials (C18 and C30). As in the following example Cholester retains planar Triphenylene longer than stereoscopic o-Therphenyl.

Planarity Selectivity (Planarity : o-Terphenyl < Triphenylene)
Cholester shows greater planarity selectivity.

 Conditions:  

Column Size	4.6 mmI.D. x 150 mm
Mobile Phase	90% Methanol
Flow Rate	1.0 ml/min
Temperature	30 oC
Detection	UV 254 nm
Sample	1. o-Terphenyl (0.10 µg) 2. Triphenylene (0.01 µg)

Characteristics of separation
Aryl Halides (Relative strengths of nucleophiles : Methyl < Chlorine < Bromine)
Cholester is more selective for Aryl Halides.

Conditions:  

Column Size	4.6 mmI.D. x 150 mm
Mobile Phase	60% Methanol
Flow Rate	1.0 ml/min
Temperature	30 oC
Detection	UV 254 nm
Sample	1. Toluene (3.3 µg) 2. Chlorobenzene (3.3 µg) 3. Bromobenzene (3.3 µg)

Steroids like D Vitamins
Cholesteryl bonded groups are very rigid structures and offer improved separation of compounds that are difficult to analyze with alkyl group bonded materials.

 Conditions:  

Column Size	4.6 mmI.D. x 150 mm
Mobile Phase	100% Methanol
Flow Rate	1.0 ml/min
Temperature	30 oC
Detection	UV 265 nm
Sample	1. Vitamin D2 (0.3 µg) 2. Vitamin D3 (0.1 µg)

Diasteromers like Stilbenes
Cholester offers improved resolution of geometric isomers.

Conditions:  

Column Size	4.6 mmI.D. x 150 mm
Mobile Phase	70% Methanol
Flow Rate	1.0 ml/min
Temperature	30 oC
Detection	UV 254 nm
Sample	1. cis-Stilbene (0.1 µg) 2. trans-Stilbene (0.2 µg)

Position isomers like Chloroacetophenones and Terphenyls
Generally isomers of disubstituted benzene are difficult to separate with C18 because their structual and polar characteristics are veryb similar. In these cases COSMOSIL Cholester works very well.

Conditions:  

Column Size	4.6 mmI.D. x 150 mm
Mobile Phase	50% Methanol
Flow Rate	1.0 ml/min
Temperature	30 oC
Detection	UV 254 nm
Sample	1. o-Chloroacetophenone (0.30 µg) 2. m-Chloroacetophenone (0.15 µg) 3. p-Chloroacetophenone (0.05 µg)

Conditions:  

1. Jomori, Takahiro, et al. "Mycobacterium smegmatis alters the production of secondary metabolites by marine-derived Aspergillus niger." Journal of Natural Medicines 74.1 (2020): 76-82.
2. Sibero, Mada Triandala, et al. "Chromanone-type compounds from marine sponge-derived Daldinia eschscholtzii KJMT FP 4.1." Journal of Applied Pharmaceutical Science 10.1 (2020): 001-007.
3. ZAIMA, Kazumasa, et al. "Application of High-Speed Countercurrent Chromatography to the Separation of Flavanonol, Phenylcoumaran and Flavonolignans in Silybum marianum." CHROMATOGRAPHY (2020).
4. Sato, Tomoyuki, et al. "Cadinane sesquiterpenoids isolated from Santalum album using a screening program for Wnt signal inhibitory activity." Journal of Natural Medicines 74.2 (2020): 476-481.
5. Takayama, Kentaro, et al. "A chemically stable peptide agonist to neuromedin U receptor type 2." Bioorganic & Medicinal Chemistry (2020): 115454.
6. Janicka, Małgorzata, Małgorzata Sztanke, and Krzysztof Sztanke. "Predicting the Blood-Brain Barrier Permeability of New Drug-Like Compounds via HPLC with Various Stationary Phases." Molecules 25.3 (2020): 487.
7. Nakayama, Hideyuki, et al. "Glucose esters of caffeic acid from Allium macrostemon Bunge." Japanese Journal of Food Chemistry and Safety 27.1 (2020): 28-32.
8. Ono, Masateru, et al. "Identification and characterization of organic and glycosidic acids in crude resin glycoside fraction from Calystegia hederacea." Journal of Natural Medicines 74.1 (2020): 200-211.
9. Kaunda, Joseph Sakah, and Ying-Jun Zhang. "Two new 23S, 26R-hydroxylated spirostanoid saponins from the fruits of Solanum indicum var. recurvatum." Steroids 153 (2020): 108506.
10. Katsutani, Kazuma, et al. "Eudesmane-type sesquiterpene glycosides: sonneratiosides A–E and eudesmol β-d-glucopyranoside from the leaves of Sonneratia alba." Journal of Natural Medicines (2020): 1-8.
11. Dougnon, Godfried, and Michiho Ito. "Medicinal uses, thin-layer chromatography and high-performance liquid chromatography profiles of plant species from Abomey-Calavi and Dantokpa Market in the Republic of Benin." Journal of Natural Medicines 74.1 (2020): 311-322.

Brochures 

• COSMOSIL Cholester  (640 KB)
• COSMOSIL 2.5Cholester  (1.2 MB)
• Analysis of Natural Compounds by 2.5Cholester (390 KB)
• Analysis of New Antimycotic Substances (5aTHQs) by COSMOSIL Cholester  (1 MB)
• COSMOSIL/COSMOCORE Cholester Series Application Notebook and Reference List  (14 MB)

• Identifying Vitamin D2 and D3 and their 25-OH Metabolites and C3 Epimers in a Single LC-MS Run (Poster from Food Science 2016, Singapore)
   (0.2 MB)
• Simultaneous Separation of Fat-Soluble Vitamins by Reversed-Phase HPLC Using a Cholesteryl Group Bonded Stationary Phase (Poster from AOAC Annual Meeting & Exposition 2013, Chicago, IL USA)
   (0.2 MB)
• Rapid HPLC Method Development with C18 and Phenyl Columns
  (Poster from HPLC2013, Amsterdam, the Netherlands)
   (0.2 MB)
• Simultaneous Separation of Fat-Soluble Vitamins by Reversed-Phase HPLC Using a Cholesteryl Group Bonded Stationary Phase
  (Poster from AOAC Annual Meeting 2012, Las Vegas, Nevada USA)
   (0.2 MB)

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