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Home > Products > Analytical Sciences > COSMOSIL HPLC/SFC Columns > Reversed Phase Specialty Columns > COSMOSIL Cholester

COSMOSIL Cholester

Specialty for Structual Isomers / Hydrophobic interaction and planarity selectivity

COSMOSIL Cholester is a new silica based RP column which employs a cholestery bonded phase. Cholester provides enhanced selectivity over traditional C18 materials and greater performance in separating isomers or other closely related compounds. Cholester is ideal for method development and serves as an excellent alternative to traditional C18 columns.

 

COSMOSIL Cholester
USP code L101
Silica gel High purity porous spherical silica
Average particle size 2.5µm 3µm 5µm
Average pore size approx. 130Å approx. 120Å approx. 120Å
Specific surface area approx. 330m2/g approx. 300m2/g approx. 300m2/g
Stationary phase Cholesteryl group
Bonding type Monomeric
End capping treatment Near-perfect treatment
Carbon content approx. 21% - approx. 20%
pH range 2 - 7.5

Hydrophobic Interaction

Separation of Alkylbenzene (comparison with hydrophobicity)
Figure shows the comparison of hydrophobic interaction with competitive C18 and C30 columns. Cholester provides the same hydrphobicity as alkyl group bonded type (C18. C30). It is not necessary to change the analytical conditions when replacing C18 or C30 columns with Cholester.

Conditions:  

Column Size 4.6 mmI.D. x 150 mm
Mobile Phase Methanol : Water = 80 : 20
Flow Rate 1.0 ml/min
Temperature 30 oC
Detection UV 254 nm
Sample
1. Benzen (1.67 µg)
2. Toluene (1.67 µg)
3. Ethylbenzene (1.67 µg)
4. Propylbenzene (1.67 µg)
5. Butylbenzene (1.67 µg)
6. Amylbenzene (1.67 µg)
 

Planarity Selectivity

The stationary phase of Cholester has very rigit structure and can distinguish different molecular shapes. Cholester offers improved separation for structurally similar compunds that are difficult to analyze with alkyl group bonded materials (C18 and C30). As in the following example Cholester retains planar Triphenylene longer than stereoscopic o-Therphenyl.

 

Planarity Selectivity (Planarity : o-Terphenyl < Triphenylene)
Cholester shows greater planarity selectivity.

 Conditions:  

Column Size 4.6 mmI.D. x 150 mm
Mobile Phase 90% Methanol
Flow Rate 1.0 ml/min
Temperature 30 oC
Detection UV 254 nm
Sample
1. o-Terphenyl (0.10 µg)
2. Triphenylene (0.01 µg)

Applications

Characteristics of separation
Aryl Halides (Relative strengths of nucleophiles : Methyl < Chlorine < Bromine)
Cholester is more selective for Aryl Halides.

Conditions:  

Column Size 4.6 mmI.D. x 150 mm
Mobile Phase 60% Methanol
Flow Rate 1.0 ml/min
Temperature 30 oC
Detection UV 254 nm
Sample
1. Toluene (3.3 µg)
2. Chlorobenzene (3.3 µg)
3. Bromobenzene (3.3 µg)
 

 

Steroids like D Vitamins
Cholesteryl bonded groups are very rigid structures and offer improved separation of compounds that are difficult to analyze with alkyl group bonded materials.

 Conditions:  

Column Size 4.6 mmI.D. x 150 mm
Mobile Phase 100% Methanol
Flow Rate 1.0 ml/min
Temperature 30 oC
Detection UV 265 nm
Sample
1. Vitamin D2 (0.3 µg)
2. Vitamin D3 (0.1 µg)
 
 
 

Diasteromers like Stilbenes
Cholester offers improved resolution of geometric isomers.

Conditions:  

Column Size 4.6 mmI.D. x 150 mm
Mobile Phase 70% Methanol
Flow Rate 1.0 ml/min
Temperature 30 oC
Detection UV 254 nm
Sample
1. cis-Stilbene (0.1 µg)
2. trans-Stilbene (0.2 µg)
 
 

Position isomers like Chloroacetophenones and Terphenyls
Generally isomers of disubstituted benzene are difficult to separate with C18 because their structual and polar characteristics are veryb similar. In these cases COSMOSIL Cholester works very well.

Conditions:  

Column Size 4.6 mmI.D. x 150 mm
Mobile Phase 50% Methanol
Flow Rate 1.0 ml/min
Temperature 30 oC
Detection UV 254 nm
Sample

1. o-Chloroacetophenone (0.30 µg)
2. m-Chloroacetophenone (0.15 µg)
3. p-Chloroacetophenone (0.05 µg)  

 
 
 
 
Catechines

Conditions:  

Column Size 4.6 mmI.D. x 150 mm
Mobile Phase

A: Acetonitrile : 20mmol/l Phosphate Buffer (pH2.5) = 10 : 90
B: Acetonitrile : 20mmol/l Phosphate Buffer (pH2.5) = 30 : 70
B 0 → 100%/20min Linear Gradient
Flow Rate 1.0 ml/min
Temperature 30 oC
Detection UV 284 nm
Sample
1. Gallocatechin (GC)
2. Caffeine
3. Epigallocatechin (EGC)
4. Catechin (C)
5. Epicatechin (EC)
6. Epigallocatechin Gallate (EGCG)
7. Gallocatechin Gallate (GCG)
8. Epicatechin Gallate (ECG)
9. Catechin Gallate (CG)

(0.80 mg/ml)
(0.08 mg/ml)
(0.80 mg/ml)
(0.40 mg/ml)
(0.40 mg/ml)
(0.20 mg/ml)
(0.40 mg/ml)
(0.20 mg/ml)
(0.20 mg/ml)
Injection Vol. 1 µl

Applications -2

NMUR2 selective agonist

 Takayama, Kentaro, et al. "A chemically stable peptide agonist to neuromedin U receptor type 2." Bioorganic & medicinal chemistry 28.10 (2020): 115454.

 

D-DLDA-DL-Amino Acids

Application data for isomers of 13-hydroxy-octadecadienoic acid

These compounds have been found to be related to several diseases, including atherosclerosis, asthma, and several types of cancer. As the risk of disease is related to the ratio of the cis/trans isomers, the ability to separate them chromatographically is important.

One of our customers has developed a method to separate two of these isomers using our COSMOSIL Cholester column. This column excels at separation of geometric isomers.

Usage Information

Column5 µm Cholester
I.D. (mm)2.03.04.610.020.028.0
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range pH2～pH7.5
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.5 µm or finer filter before use.

Downloads

Brochures 

  • COSMOSIL Cholester pdf (640 KB)
  • COSMOSIL 2.5Cholester pdf (1.2 MB)
  • Analysis of Natural Compounds by 2.5Cholesterpdf (390 KB)
  • Analysis of New Antimycotic Substances (5aTHQs) by COSMOSIL Cholester pdf (1 MB)
  • COSMOSIL/COSMOCORE Cholester Series Application Notebook and Reference List pdf (14 MB)
 
Posters 
  • Identifying Vitamin D2 and D3 and their 25-OH Metabolites and C3 Epimers in a Single LC-MS Run (Poster from Food Science 2016, Singapore)
    pdf (0.2 MB)
  • Simultaneous Separation of Fat-Soluble Vitamins by Reversed-Phase HPLC Using a Cholesteryl Group Bonded Stationary Phase (Poster from AOAC Annual Meeting & Exposition 2013, Chicago, IL USA)
    pdf (0.2 MB)
  • Rapid HPLC Method Development with C18 and Phenyl Columns
    (Poster from HPLC2013, Amsterdam, the Netherlands)
    pdf (0.2 MB)
  • Simultaneous Separation of Fat-Soluble Vitamins by Reversed-Phase HPLC Using a Cholesteryl Group Bonded Stationary Phase
    (Poster from AOAC Annual Meeting 2012, Las Vegas, Nevada USA)
    pdf (0.2 MB)

 

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size. 

>> COSMOSIL Application Search

Reference

  1. Jomori, Takahiro, et al. "Mycobacterium smegmatis alters the production of secondary metabolites by marine-derived Aspergillus niger." Journal of Natural Medicines 74.1 (2020): 76-82.
  2. Sibero, Mada Triandala, et al. "Chromanone-type compounds from marine sponge-derived Daldinia eschscholtzii KJMT FP 4.1." Journal of Applied Pharmaceutical Science 10.1 (2020): 001-007.
  3. ZAIMA, Kazumasa, et al. "Application of High-Speed Countercurrent Chromatography to the Separation of Flavanonol, Phenylcoumaran and Flavonolignans in Silybum marianum." CHROMATOGRAPHY (2020).
  4. Sato, Tomoyuki, et al. "Cadinane sesquiterpenoids isolated from Santalum album using a screening program for Wnt signal inhibitory activity." Journal of Natural Medicines 74.2 (2020): 476-481.
  5. Takayama, Kentaro, et al. "A chemically stable peptide agonist to neuromedin U receptor type 2." Bioorganic & Medicinal Chemistry (2020): 115454.
  6. Janicka, Małgorzata, Małgorzata Sztanke, and Krzysztof Sztanke. "Predicting the Blood-Brain Barrier Permeability of New Drug-Like Compounds via HPLC with Various Stationary Phases." Molecules 25.3 (2020): 487.
  7. Nakayama, Hideyuki, et al. "Glucose esters of caffeic acid from Allium macrostemon Bunge." Japanese Journal of Food Chemistry and Safety 27.1 (2020): 28-32.
  8. Ono, Masateru, et al. "Identification and characterization of organic and glycosidic acids in crude resin glycoside fraction from Calystegia hederacea." Journal of Natural Medicines 74.1 (2020): 200-211.
  9. Kaunda, Joseph Sakah, and Ying-Jun Zhang. "Two new 23S, 26R-hydroxylated spirostanoid saponins from the fruits of Solanum indicum var. recurvatum." Steroids 153 (2020): 108506.
  10. Katsutani, Kazuma, et al. "Eudesmane-type sesquiterpene glycosides: sonneratiosides A–E and eudesmol β-d-glucopyranoside from the leaves of Sonneratia alba." Journal of Natural Medicines (2020): 1-8.
  11. Dougnon, Godfried, and Michiho Ito. "Medicinal uses, thin-layer chromatography and high-performance liquid chromatography profiles of plant species from Abomey-Calavi and Dantokpa Market in the Republic of Benin." Journal of Natural Medicines 74.1 (2020): 311-322.

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size

Ordering Information

Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 2.5 Cholester Packed Column (2.0 mmI.D. x 50 mm) 09000-01 Waters type 1 pkg 728.00 Buy
COSMOSIL 2.5 Cholester Packed Column (2.0 mmI.D. x 75 mm) 09047-11 Waters type 1 pkg 728.00 Buy
COSMOSIL 2.5 Cholester Packed Column (2.0 mmI.D. x 100 mm) 09048-01 Waters type 1 pkg 728.00 Buy
COSMOSIL 2.5 Cholester Packed Column (3.0 mmI.D. x 50 mm) 09049-91 Waters type 1 pkg 728.00 Buy
COSMOSIL 2.5 Cholester Packed Column (3.0 mmI.D. x 75 mm) 09050-51 Waters type 1 pkg 728.00 Buy
COSMOSIL 2.5 Cholester Packed Column (3.0 mmI.D. x 100 mm) 09051-41 Waters type 1 pkg 728.00 Buy
COSMOSIL 2.5 Cholester Packed Column (3.0 mmI.D. x 150 mm) 07099-01 Waters type 1 pkg 878.00 Buy
COSMOSIL 2.5 Cholester Packed Column (4.6 mmI.D. x 150 mm) 13838-21 Waters type 1 pkg 878.00 Buy
COSMOSIL 3Cholester Packed Column (2.0 mmI.D. x 50 mm) 19188-61 Waters type 1 pkg 664.00 Buy
COSMOSIL 3Cholester Packed Column (2.0 mmI.D. x 75 mm) 19189-51 Waters type 1 pkg 664.00 Buy
COSMOSIL 3Cholester Packed Column (2.0 mmI.D. x 100 mm) 19190-11 Waters type 1 pkg 664.00 Buy
COSMOSIL 3Cholester Packed Column (2.0 mmI.D. x 150 mm) 19191-01 Waters type 1 pkg 664.00 Buy
COSMOSIL 3Cholester Packed Column (2.0 mmI.D. x 250 mm) 19192-91 Waters type 1 pkg 792.00 Buy
COSMOSIL 3Cholester Packed Column (3.0 mmI.D. x 50 mm) 19194-71 Waters type 1 pkg 664.00 Buy
COSMOSIL 3Cholester Packed Column (3.0 mmI.D. x 75 mm) 19195-61 Waters type 1 pkg 664.00 Buy
COSMOSIL 3Cholester Packed Column (3.0 mmI.D. x 100 mm) 19196-51 Waters type 1 pkg 664.00 Buy
COSMOSIL 3Cholester Packed Column (3.0 mmI.D. x 150 mm) 19197-41 Waters type 1 pkg 664.00 Buy
COSMOSIL 3Cholester Packed Column (3.0 mmI.D. x 250 mm) 19198-31 Waters type 1 pkg 792.00 Buy
COSMOSIL 3Cholester Packed Column (4.6 mmI.D. x 50 mm) 19199-21 Waters type 1 pkg 664.00 Buy
COSMOSIL 3Cholester Packed Column (4.6 mmI.D. x 75 mm) 19200-71 Waters type 1 pkg 664.00 Buy
COSMOSIL 3Cholester Packed Column (4.6 mmI.D. x 100 mm) 19300-61 Waters type 1 pkg 632.00 Buy
COSMOSIL 3Cholester Packed Column (4.6 mmI.D. x 150 mm) 19151-21 Waters type 1 pkg 632.00 Buy
COSMOSIL 3Cholester Packed Column (4.6 mmI.D. x 250 mm) 19316-71 Waters type 1 pkg 739.00 Buy
COSMOSIL 5Cholester Packed Column (1.0 mm I.D. x 150 mm) 05968-71 Waters type 1 pkg 637.00 Buy
COSMOSIL 5Cholester Packed Column (1.0 mm I.D. x 250 mm) 05969-61 Waters type 1 pkg 749.00 Buy
COSMOSIL 5Cholester Packed Column (2.0 mm I.D. x 30 mm) 08565-51 Waters type 1 pkg 509.00 Buy
COSMOSIL 5Cholester Packed Column (2.0 mm I.D. x 50 mm) 06352-91 Waters type 1 pkg 594.00 Buy
COSMOSIL 5Cholester Packed Column (2.0 mm I.D. x 100 mm) 06948-01 Waters type 1 pkg 541.00 Buy
COSMOSIL 5Cholester Packed Column (2.0 mm I.D. x 150 mm) 05971-11 Waters type 1 pkg 546.00 Buy
COSMOSIL 5Cholester Packed Column (2.0 mm I.D. x 250 mm) 05972-01 Waters type 1 pkg 659.00 Buy
COSMOSIL 5Cholester Packed Column (3.0 mm I.D. x 100 mm) 15892-41 Waters type 1 pkg N/A Inquire
COSMOSIL 5Cholester Packed Column (3.0 mm I.D. x 150 mm) 05973-91 Waters type 1 pkg 509.00 Buy
COSMOSIL 5Cholester Packed Column (3.0 mm I.D. x 250 mm) 05974-81 Waters type 1 pkg 578.00 Buy
COSMOSIL 5Cholester Packed Column (4.6 mm I.D. x 50 mm) 06359-21 Waters type 1 pkg 573.00 Buy
COSMOSIL 5Cholester Packed Column (4.6 mm I.D. x 100 mm) 06591-61 Waters type 1 pkg 541.00 Buy
COSMOSIL 5Cholester Packed Column (4.6 mm I.D. x 150 mm) 05976-61 Waters type 1 pkg 509.00 Buy
COSMOSIL 5Cholester Packed Column (3 Lots Set) (4.6 mm I.D. x 150 mm) 07970-03 Waters type 3 pkg 1,520.00 Buy
COSMOSIL 5Cholester Packed Column (4.6 mm I.D. x 250 mm) 05977-51 Waters type 1 pkg 552.00 Buy
COSMOSIL 5Cholester Packed Column (6.0 mm I.D. x 150 mm) 06445-81 Waters type 1 pkg 696.00 Buy
COSMOSIL 5Cholester Packed Column (10.0 mm I.D. x 50 mm) 16590-61 Waters type 1 pkg 899.00 Buy
COSMOSIL 5Cholester Packed Column (10.0 mm I.D. x 100 mm) 16591-51 Waters type 1 pkg 1,017.00 Buy
COSMOSIL 5Cholester Packed Column (10.0 mm I.D. x 150 mm) 08011-91 Waters type 1 pkg 1,070.00 Buy
COSMOSIL 5Cholester Packed Column (10.0 mm I.D. x 250 mm) 05979-31 Waters type 1 pkg 1,359.00 Buy
COSMOSIL 5Cholester Packed Column (10.0 mm I.D. x 100 mm) 15995-01 Waters type 1 pkg 2,039.00 Buy
COSMOSIL 5Cholester Packed Column (20.0 mm I.D. x 150 mm) 06088-71 Waters type 1 pkg 2,146.00 Buy
COSMOSIL 5Cholester Packed Column (20.0 mm I.D. x 250 mm) 05982-71 Waters type 1 pkg 2,816.00 Buy
COSMOSIL 5Cholester Packed Column (28.0 mm I.D. x 100 mm) 16592-41 Waters type 1 pkg 5,725.00 Buy
COSMOSIL 5Cholester Packed Column (28.0 mm I.D. x 150 mm) 16593-31 Waters type 1 pkg 6,025.00 Buy
COSMOSIL 5Cholester Packed Column (28.0 mm I.D. x 250 mm) 05985-41 Waters type 1 pkg 6,190.00 Buy
Guard Column / Guard Cartridge
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 3Cholester Guard Cartridge (2.0 mmI.D. x 10 mm) 19325-64 Waters type 2 pkg 236.00 Buy
COSMOSIL 3Cholester Guard Cartridge (4.6 mmI.D. x 10 mm) 19344-14 Waters type 2 pkg 236.00 Buy
COSMOSIL 5Cholester Guard Column (3.0 mm I.D. x 10 mm) 06175-21 Waters type 1 pkg N/A Inquire
COSMOSIL 5Cholester Guard Cartridge (4.6 mm I.D. x 10 mm) 19183-24 Waters type 2 pkg 236.00 Buy
COSMOSIL 5Cholester Guard Column (10.0 mm I.D. x 20 mm) 05978-41 Waters type 1 pkg 509.00 Buy
COSMOSIL 5Cholester Guard Column (20.0 mm I.D. x 20 mm) 05980-91 Waters type 1 pkg 1,124.00 Buy
COSMOSIL 5Cholester Guard Column (20.0 mm I.D. x 50 mm) 05981-81 Waters type 1 pkg 1,690.00 Buy
COSMOSIL 5Cholester Guard Column (28.0 mm I.D. x 50 mm) 05983-61 Waters type 1 pkg 3,697.00 Buy
HPLC Accessories
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL Direct Cartridge Holder 19989-71 Waters type 1 pkg 270.00 Buy
COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) **Discontinued** 38009-79 Waters type 1 pkg N/A Inquire
COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) 11884-71 Waters type 1 pkg 265.00 Buy
COSMOSIL Column Prefilter 39361-19 Waters type 1 pkg 145.00 Buy
COSMOSIL Column Spare Filter for Prefilter (1 micron) 39539-09 Waters type 2 pkg 55.00 Buy
COSMOSIL Column Connecting Tube (0.25 mm) 37843-69 Waters type 1 pkg 65.00 Buy
COSMOSIL Column Connecting Tube (0.1 mm) 12570-41 Waters type 1 pkg 65.00 Buy

** Guard cartridges require a guard cartridge holder.
** #38009-79 has been discontinued. Alternative product is COSMOSIL Direct Cartridge Holder (#19989-71).

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