Specialty for Structual Isomers / π-π interaction, excellent selectivity for unsaturated compounds
Separation of unsaturated compounds on conventional reversed phase columns such as C8 and C18 is often problematic. Phenyl stationary phases are commonly used for these compounds because they form π-π interactions and show different selectivity from conventional alkyl phases. However, the π-π interactions on phenyl stationary phases are moderate, and the separation for some unsaturated compounds are still insufficient.
COSMOSIL πNAP is a Reversed Phase HPLC Column with naphtylethyl group bonded silica packing material. The naphthylethyl group is composed of two fused aromatic rings and forms strong π-π interactions with unsaturated compounds. This column offers improved selectivity for structural isomers that exhibit differences in saturation.
COSMOSIL πNAP | |||
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Silica gel | High purity porous spherical silica | ||
Average particle size | 2.5 µm | 3 µm | 5 µm |
Average pore size | approx. 130 Å | approx. 120 Å | approx. 120Å |
Specific surface area | approx. 330m2/g | approx. 300 m²/g | approx. 300 m²/g |
Stationary phase | Naphtylethyl group | ||
Bonding type | Monomeric | ||
Carbon content | approx. 11% | - | approx. 11% |
Usable pH range | 2 - 7.5 |
COSMOSIL πNAP shows stronger π-π interactions than phenyl columns. Its two fused aromatic rings retain nitrobenzene with more π ekectrons stronger than phenyl columns.
Technical Note: Analysis of Cisplatin and its Variants in Water using COSMOSIL piNAP (PDF)
COSMOSIL πNAP uses a naphthalene-based bonded phase, and exhibits different retention behavior from C18. In this note, πNAP is used to analyze cisplatin (CDDP) and its mono-chloro, none-chloro (hydration complex), and OH-dimer forms in water.
Standard C18 columns have difficulty separating compounds of similar hydrophobicity. Perfomance may be improved by using Cholester, a column with improved molecular shape recognition.
Cisplatin (CDDP) in water converts to its mono-chloro, hydration complex, and OH-dimer forms, and it is thought that they exist in an equilibrium state (fig. 1). Each of these forms was separated and quantified.
Fig. 1. Equilibrium reactions of cisplatin (CDDP) in water
In water, CDDP loses Cl-, which is replaced with water. Depending on the pH, it may be replaced by a hydroxide ion. The mono-chloro form may lose another Cl-, and, in the same way, the water molecules (one or both) may be replaced with a hydroxide ion. These forms are in equilibrium.
Comparing a C18 column, COSMOSIL Cholester, and πNAP under identical conditions
When measuring the OH-dimer nitrate form, the dissociated NO3- peak elutes before the OH-dimer peak. C18-MS-II could not separate the two peaks, and Cholester achieved partial separation, but could not separate to baseline. πNAP, however, was able to separate the compounds completely.
Analysis Using COSMOSIL πNAP
Below is a typical chromatogram; the peak separation was excellent. The calibration curve for cisplatin (CDDP) was linear in the range of 0.01 to 4 mM, and the CV values for within-day and between-day variation were within 5%.
By using COSMOSIL πNAP, it is possible to separate and quantify cisplatin (CDDP) and its mono-chloro, none-chloro, and OH-dimer forms using HPLC.
Data courtesy of Prof. Ryuji Kato, Ph D., Laboratory of Cardiovascular Pharmacotherapy and Toxicology, Osaka University of Pharmaceutical Sciences
Many of our columns feature aromatic ring-based stationary phases with strong π-π interactions, notably our πNAP (naphthylethyl) and PYE (pyrenylethyl) columns. Several other companies produce similar columns using a biphenyl phase, which has become a popular alternative to C18 in recent years. Here, we compare these columns for several kinds of analysis.
Column | 5 µm πNAP | |||||
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I.D. (mm) | 2.0 | 3.0 | 4.6 | 10.0 | 20.0 | 28.0 |
Washing method | 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. 2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF. |
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Storage conditions | Short-term (up to a week): Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. Long-term: Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water. In either case, tightly plug the column, and store in a cool and dry place. |
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Usable pH range | pH2~pH7.5 | |||||
Max. pressure | 20MPa | 15MPa | ||||
Temperature range | The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C. | |||||
Usable solvents | Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable. Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points. |
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Other instructions | - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. - Always filter mobile phases using a 0.5 µm or finer filter before use. - Acetonitrile is not recommended as a mobile phase. - Baseline drift may occur. |
COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size
New Application: Sesamin metabolites
Product | Description | Cat.No. | PKG Size | Price | |
---|---|---|---|---|---|
COSMOSIL 2.5πNAP Packed Column (2.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
06062-91 | 1 pkg | 803.00 | Buy |
COSMOSIL 2.5πNAP Packed Column (2.0 mm I.D. x 75 mm) | End-Fitting: Waters type |
06051-31 | 1 pkg | 803.00 | Buy |
COSMOSIL 2.5πNAP Packed Column (2.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
06052-21 | 1 pkg | 803.00 | Buy |
COSMOSIL 2.5πNAP Packed Column (2.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
19079-91 | 1 pkg | 963.00 | Buy |
COSMOSIL 2.5πNAP Packed Column (3.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
06054-01 | 1 pkg | 803.00 | Buy |
COSMOSIL 2.5πNAP Packed Column (3.0 mm I.D. x 75 mm) | End-Fitting: Waters type |
06055-91 | 1 pkg | 803.00 | Buy |
COSMOSIL 2.5πNAP Packed Column (3.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
06057-71 | 1 pkg | 803.00 | Buy |
COSMOSIL 2.5πNAP Packed Column (3.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
15632-41 | 1 pkg | 963.00 | Buy |
COSMOSIL 3πNAP Packed Column (2.0 mmI.D. x 50 mm) | End-Fitting: Waters type |
21495-61 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (2.0 mmI.D. x 75 mm) | End-Fitting: Waters type |
21496-51 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (2.0 mmI.D. x 100 mm) | End-Fitting: Waters type |
21497-41 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (2.0 mmI.D. x 150 mm) | End-Fitting: Waters type |
21499-21 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (2.0 mmI.D. x 250 mm) | End-Fitting: Waters type |
21500-71 | 1 pkg | 878.00 | Buy |
COSMOSIL 3πNAP Packed Column (3.0 mmI.D. x 50 mm) | End-Fitting: Waters type |
21509-81 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (3.0 mmI.D. x 75 mm) | End-Fitting: Waters type |
21513-11 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (3.0 mmI.D. x 100 mm) | End-Fitting: Waters type |
21514-01 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (3.0 mmI.D. x 150 mm) | End-Fitting: Waters type |
21520-11 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (3.0 mmI.D. x 250 mm) | End-Fitting: Waters type |
21521-01 | 1 pkg | 878.00 | Buy |
COSMOSIL 3πNAP Packed Column (4.6 mmI.D. x 50 mm) | End-Fitting: Waters type |
21522-91 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (4.6 mmI.D. x 75 mm) | End-Fitting: Waters type |
21523-81 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (4.6 mmI.D. x 100 mm) | End-Fitting: Waters type |
21524-71 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (4.6 mmI.D. x 150 mm) | End-Fitting: Waters type |
21525-61 | 1 pkg | 728.00 | Buy |
COSMOSIL 3πNAP Packed Column (4.6 mmI.D. x 250 mm) | End-Fitting: Waters type |
21526-51 | 1 pkg | 878.00 | Buy |
COSMOSIL 5πNAP Packed Column (1.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
08076-61 | 1 pkg | 846.00 | Buy |
COSMOSIL 5πNAP Packed Column (1.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
08077-51 | 1 pkg | 990.00 | Buy |
COSMOSIL 5πNAP Packed Column (2.0 mm I.D. x 30 mm) | End-Fitting: Waters type |
08566-41 | 1 pkg | 562.00 | Buy |
COSMOSIL 5πNAP Packed Column (2.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
08567-31 | 1 pkg | 594.00 | Buy |
COSMOSIL 5πNAP Packed Column (2.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
08299-51 | 1 pkg | 685.00 | Buy |
COSMOSIL 5πNAP Packed Column (2.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
08078-41 | 1 pkg | 744.00 | Buy |
COSMOSIL 5πNAP Packed Column (2.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
08079-31 | 1 pkg | 878.00 | Buy |
COSMOSIL 5πNAP Packed Column (3.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
08080-91 | 1 pkg | 621.00 | Buy |
COSMOSIL 5πNAP Packed Column (3.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
08081-81 | 1 pkg | 787.00 | Buy |
COSMOSIL 5πNAP Packed Column (4.6 mm I.D. x 50 mm) | End-Fitting: Waters type |
08083-61 | 1 pkg | 493.00 | Buy |
COSMOSIL 5πNAP Packed Column (4.6 mm I.D. x 100 mm) | End-Fitting: Waters type |
08084-51 | 1 pkg | 584.00 | Buy |
COSMOSIL 5πNAP Packed Column (4.6 mm I.D. x 150 mm) | End-Fitting: Waters type |
08085-41 | 1 pkg | 621.00 | Buy |
COSMOSIL 5πNAP Packed Column (4.6 mm I.D. x 250 mm) | End-Fitting: Waters type |
08086-31 | 1 pkg | 787.00 | Buy |
COSMOSIL 5πNAP Packed Column (6.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
08249-01 | 1 pkg | 749.00 | Buy |
COSMOSIL 5πNAP Packed Column (6.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
08248-11 | 1 pkg | 910.00 | Buy |
COSMOSIL 5πNAP Packed Column (10.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
16594-21 | 1 pkg | 899.00 | Buy |
COSMOSIL 5πNAP Packed Column (10.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
16595-11 | 1 pkg | 1,263.00 | Buy |
COSMOSIL 5πNAP Packed Column (10.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
08088-11 | 1 pkg | 1,461.00 | Buy |
COSMOSIL 5πNAP Packed Column (10.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
08089-01 | 1 pkg | 1,980.00 | Buy |
COSMOSIL 5πNAP Packed Column (20.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
16596-01 | 1 pkg | 2,772.00 | Buy |
COSMOSIL 5πNAP Packed Column (20.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
08092-41 | 1 pkg | 2,932.00 | Buy |
COSMOSIL 5πNAP Packed Column (20.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
08093-31 | 1 pkg | 3,965.00 | Buy |
COSMOSIL 5πNAP Packed Column (28.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
12563-41 | 1 pkg | 6,206.00 | Buy |
COSMOSIL 5πNAP Packed Column (28.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
16597-91 | 1 pkg | 6,313.00 | Buy |
COSMOSIL 5πNAP Packed Column (28.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
08095-11 | 1 pkg | 7,897.00 | Buy |
Product | Description | Cat.No. | PKG Size | Price | |
---|---|---|---|---|---|
COSMOSIL 3πNAP Guard Cartridge (4.6 mm I.D. x 10 mm) | End-Fitting: Waters type |
21527-54 | 2 pkg | 257.00 | Buy |
COSMOSIL 5πNAP Guard Column (4.6 mm I.D. x 10 mm) | End-Fitting: Waters type |
08082-71 | 1 pkg | 295.00 | Buy |
COSMOSIL 5πNAP Guard Column (10.0 mm I.D. x 20 mm) | End-Fitting: Waters type |
08087-21 | 1 pkg | 562.00 | Buy |
COSMOSIL 5πNAP Guard Column (20.0 mm I.D. x 20 mm) | End-Fitting: Waters type |
08090-61 | 1 pkg | 1,215.00 | Buy |
COSMOSIL 5πNAP Guard Column (20.0 mm I.D. x 50 mm) | Capacity: 08091-51 End-Fitting: Waters type |
08091-51 | 1 pkg | 1,839.00 | Buy |
COSMOSIL 5πNAP Guard Column (28.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
08094-21 | 1 pkg | 3,820.00 | Buy |
Product | Description | Cat.No. | PKG Size | Price | |
---|---|---|---|---|---|
COSMOSIL Direct Cartridge Holder | End-Fitting: Waters type |
19989-71 | 1 pkg | 270.00 | Buy |
COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) **Discontinued** | End-Fitting: Waters type |
38009-79 | 1 pkg | - | Inquire |
COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) | End-Fitting: Waters type |
11884-71 | 1 pkg | 265.00 | Buy |
COSMOSIL Column Prefilter | End-Fitting: Waters type |
39361-19 | 1 pkg | 145.00 | Buy |
COSMOSIL Column Spare Filter for Prefilter (1 micron) | End-Fitting: Waters type |
39539-09 | 2 pkg | 55.00 | Buy |
COSMOSIL Column Connecting Tube (0.25 mm) | End-Fitting: Waters type |
37843-69 | 1 pkg | 65.00 | Buy |
COSMOSIL Column Connecting Tube (0.1 mm) | End-Fitting: Waters type |
12570-41 | 1 pkg | 65.00 | Buy |
#38009-79 has been discontinued. Alternative product is COSMOSIL Direct Cartridge Holder (#19989-71).