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COSMOSIL πNAP

Specialty for Structual Isomers / π-π interaction, excellent selectivity for unsaturated compounds

Separation of unsaturated compounds on conventional reversed phase columns such as C8 and C18 is often problematic. Phenyl stationary phases are commonly used for these compounds because they form π-π interactions and show different selectivity from conventional alkyl phases. However, the π-π interactions on phenyl stationary phases are moderate, and the separation for some unsaturated compounds are still insufficient.

COSMOSIL πNAP is a Reversed Phase HPLC Column with naphtylethyl group bonded silica packing material. The naphthylethyl group is composed of two fused aromatic rings and forms strong π-π interactions with unsaturated compounds. This column offers improved selectivity for structural isomers that exhibit differences in saturation.

COSMOSIL πNAP
Silica gel High purity porous spherical silica
Average particle size 2.5 µm 5 µm
Average pore size approx. 130 Å approx. 120Å
Specific surface area approx. 330m2/g approx. 300 m²/g
Stationary phase Naphtylethyl group Naphtylethyl group
Bonding type Monomeric Monomeric
Carbon content approx. 11% approx. 11%
Usable pH range 2 - 7.5 2 - 7.5

Figure 1. Comparison of π-π interactions

COSMOSIL πNAP shows stronger π-π interactions than phenyl columns

Figure 2. Separation of tolunitrile isomers

Figure 3. Separation of steroids

Figure 4. Separation of tocopherols

Analysis of Cisplatin and its Variants in Water

Technical NoteAnalysis of Cisplatin and its Variants in Water using COSMOSIL piNAP (PDF)

COSMOSIL πNAP uses a naphthalene-based bonded phase, and exhibits different retention behavior from C18. In this note, πNAP is used to analyze cisplatin (CDDP) and its mono-chloro, none-chloro (hydration complex), and OH-dimer forms in water.

Standard C18 columns have difficulty separating compounds of similar hydrophobicity. Perfomance may be improved by using Cholester, a column with improved molecular shape recognition.

Experiment Summary

Cisplatin (CDDP) in water converts to its mono-chloro, hydration complex, and OH-dimer forms, and it is thought that they exist in an equilibrium state (fig. 1). Each of these forms was separated and quantified.

Fig. 1. Equilibrium reactions of cisplatin (CDDP) in water
In water, CDDP loses Cl-, which is replaced with water. Depending on the pH, it may be replaced by a hydroxide ion. The mono-chloro form may lose another Cl-, and, in the same way, the water molecules (one or both) may be replaced with a hydroxide ion. These forms are in equilibrium.

Quantification of Cisplatin (CDDP) and its Mono-Chloro, None-Chloro, and OH-Dimer Forms

Comparing a C18 column, COSMOSIL Cholester, and πNAP under identical conditions

When measuring the OH-dimer nitrate form, the dissociated NO3- peak elutes before the OH-dimer peak. C18-MS-II could not separate the two peaks, and Cholester achieved partial separation, but could not separate to baseline. πNAP, however, was able to separate the compounds completely.

 

Analysis Using COSMOSIL πNAP

Below is a typical chromatogram; the peak separation was excellent. The calibration curve for cisplatin (CDDP) was linear in the range of 0.01 to 4 mM, and the CV values for within-day and between-day variation were within 5%.

 

Observations

By using COSMOSIL πNAP, it is possible to separate and quantify cisplatin (CDDP) and its mono-chloro, none-chloro, and OH-dimer forms using HPLC.

References

Kato R. et al. A Novel Analytical Method of Cisplatin Using the HPLC with a Naphthylethyl Group Bonded with Silica Gel (πNAP) Column. Biol Pharm Bull. 40(3), 290-296(2017). 

Data courtesy of Prof. Ryuji Kato, Ph D., Laboratory of Cardiovascular Pharmacotherapy and Toxicology, Osaka University of Pharmaceutical Sciences

Applications

Phthalonitriles

 

Tocopherols

 

Nucleobases and Nucleosides

 

Sulfone Selectivity

 

Xanthine Derivatives (SFC)

 

Polyethylene Glycols (SFC)

Downloads

Brochures
Posters
  • Evaluation of Pentabromobenzyl Stationary Phase for Separation of Polar Compounds by Reversed-Phase Chromatography
    (Poster from HPLC 2015, Geneva)
    pdf (370 KB)
  • STUDY OF ENHANCED π-π INTERACTIONS ON REVERSED PHASE HPLC COLUMNS.
    (Poster from HPLC 2008, Baltimore, MD)
    pdf 

Usage Information

Column5 µm πNAP
I.D. (mm)2.03.04.610.020.028.0
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.
2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range pH2~pH7.5
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.5 µm or finer filter before use.
- Acetonitrile is not recommended as a mobile phase.
- Baseline drift may occur.

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size

Ordering Information

Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 2.5πNAP Packed Column (2.0 mm I.D. x 50 mm) 06062-91 Waters type 1 pkg 750.00 Buy
COSMOSIL 2.5πNAP Packed Column (2.0 mm I.D. x 75 mm) 06051-31 Waters type 1 pkg 750.00 Buy
COSMOSIL 2.5πNAP Packed Column (2.0 mm I.D. x 100 mm) 06052-21 Waters type 1 pkg 750.00 Buy
COSMOSIL 2.5πNAP Packed Column (2.0 mm I.D. x 150 mm) 19079-91 Waters type 1 pkg 900.00 Buy
COSMOSIL 2.5πNAP Packed Column (3.0 mm I.D. x 50 mm) 06054-01 Waters type 1 pkg 750.00 Buy
COSMOSIL 2.5πNAP Packed Column (3.0 mm I.D. x 75 mm) 06055-91 Waters type 1 pkg 750.00 Buy
COSMOSIL 2.5πNAP Packed Column (3.0 mm I.D. x 100 mm) 06057-71 Waters type 1 pkg 750.00 Buy
COSMOSIL 2.5πNAP Packed Column (3.0 mm I.D. x 150 mm) 15632-41 Waters type 1 pkg 900.00 Buy
COSMOSIL 5πNAP Packed Column (1.0 mm I.D. x 150 mm) 08076-61 Waters type 1 pkg 790.00 Buy
COSMOSIL 5πNAP Packed Column (1.0 mm I.D. x 250 mm) 08077-51 Waters type 1 pkg 925.00 Buy
COSMOSIL 5πNAP Packed Column (2.0 mm I.D. x 30 mm) 08566-41 Waters type 1 pkg 525.00 Buy
COSMOSIL 5πNAP Packed Column (2.0 mm I.D. x 50 mm) 08567-31 Waters type 1 pkg 555.00 Buy
COSMOSIL 5πNAP Packed Column (2.0 mm I.D. x 100 mm) 08299-51 Waters type 1 pkg 640.00 Buy
COSMOSIL 5πNAP Packed Column (2.0 mm I.D. x 150 mm) 08078-41 Waters type 1 pkg 695.00 Buy
COSMOSIL 5πNAP Packed Column (2.0 mm I.D. x 250 mm) 08079-31 Waters type 1 pkg 820.00 Buy
COSMOSIL 5πNAP Packed Column (3.0 mm I.D. x 150 mm) 08080-91 Waters type 1 pkg 580.00 Buy
COSMOSIL 5πNAP Packed Column (3.0 mm I.D. x 250 mm) 08081-81 Waters type 1 pkg 735.00 Buy
COSMOSIL 5πNAP Packed Column (4.6 mm I.D. x 50 mm) 08083-61 Waters type 1 pkg 460.00 Buy
COSMOSIL 5πNAP Packed Column (4.6 mm I.D. x 100 mm) 08084-51 Waters type 1 pkg 545.00 Buy
COSMOSIL 5πNAP Packed Column (4.6 mm I.D. x 150 mm) 08085-41 Waters type 1 pkg 580.00 Buy
COSMOSIL 5πNAP Packed Column (4.6 mm I.D. x 250 mm) 08086-31 Waters type 1 pkg 735.00 Buy
COSMOSIL 5πNAP Packed Column (6.0 mm I.D. x 150 mm) 08249-01 Waters type 1 pkg 700.00 Buy
COSMOSIL 5πNAP Packed Column (6.0 mm I.D. x 250 mm) 08248-11 Waters type 1 pkg 850.00 Buy
COSMOSIL 5πNAP Packed Column (10.0 mm I.D. x 50 mm) 16594-21 Waters type 1 pkg 840.00 Buy
COSMOSIL 5πNAP Packed Column (10.0 mm I.D. x 100 mm) 16595-11 Waters type 1 pkg 1,180.00 Buy
COSMOSIL 5πNAP Packed Column (10.0 mm I.D. x 150 mm) 08088-11 Waters type 1 pkg 1,365.00 Buy
COSMOSIL 5πNAP Packed Column (10.0 mm I.D. x 250 mm) 08089-01 Waters type 1 pkg 1,850.00 Buy
COSMOSIL 5πNAP Packed Column (20.0 mm I.D. x 100 mm) 16596-01 Waters type 1 pkg 2,590.00 Buy
COSMOSIL 5πNAP Packed Column (20.0 mm I.D. x 150 mm) 08092-41 Waters type 1 pkg 2,740.00 Buy
COSMOSIL 5πNAP Packed Column (20.0 mm I.D. x 250 mm) 08093-31 Waters type 1 pkg 3,705.00 Buy
COSMOSIL 5πNAP Packed Column (28.0 mm I.D. x 100 mm) 12563-41 Waters type 1 pkg 5,800.00 Buy
COSMOSIL 5πNAP Packed Column (28.0 mm I.D. x 150 mm) 16597-91 Waters type 1 pkg 5,900.00 Buy
COSMOSIL 5πNAP Packed Column (28.0 mm I.D. x 250 mm) 08095-11 Waters type 1 pkg 7,380.00 Buy
Guard Column
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 5πNAP Guard Column (4.6 mm I.D. x 10 mm) 08082-71 Waters type 1 pkg 275.00 Buy
COSMOSIL 5πNAP Guard Column (10.0 mm I.D. x 20 mm) 08087-21 Waters type 1 pkg 525.00 Buy
COSMOSIL 5πNAP Guard Column (20.0 mm I.D. x 20 mm) 08090-61 Waters type 1 pkg 1,135.00 Buy
COSMOSIL 5πNAP Guard Column (20.0 mm I.D. x 50 mm) 08091-51 Waters type 1 pkg 1,470.00 Buy
COSMOSIL 5πNAP Guard Column (28.0 mm I.D. x 50 mm) 08094-21 Waters type 1 pkg 3,570.00 Buy
HPLC Accessories
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL Direct Cartridge Holder 19989-71 Waters type 1 pkg 270.00 Buy
COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) 38009-79 Waters type 1 pkg 265.00 Buy
COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) 11884-71 Waters type 1 pkg 265.00 Buy
COSMOSIL Column Prefilter 39361-19 Waters type 1 pkg 145.00 Buy
COSMOSIL Column Spare Filter for Prefilter (1 micron) 39539-09 Waters type 2 pkg 55.00 Buy
COSMOSIL Column Connecting Tube (0.25 mm) 37843-69 Waters type 1 pkg 65.00 Buy
COSMOSIL Column Connecting Tube (0.1 mm) 12570-41 Waters type 1 pkg 65.00 Buy