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COSMOSIL PBr

Separation of hydrophilic compounds in reversed-phase conditions

  • Separate hydrophilic compounds in reversed-phase conditions
  • Simple mobile phase condition compared to HILIC
  • More sample loading capacity than HILIC
  • Alternative selectivity to C18 column

COSMOSIL PBr
Silica gel High purity porous spherical silica
Average particle size 3 µm            5 µm
Average pore size approx. 120Å
Specific surface area approx. 300 m²/g
Stationary phase Pentabromobenzyl group
Bonding type Monomeric
Endcapping treatment Yes
Carbon content - approx. 8%
Usable pH range 2 - 7.5

Separation of hydrophilic compounds in reversed-phase conditions

Hydrophilic interaction chromatography (HILIC) is an increasingly popular analytical technique. However, it is often difficult to develop a robust method due to users' less familiarity with HILIC conditions. Furthermore, high concentration of acetonitrile used in HILIC mobile phase makes it extremely sensitive to samples' water concentration; injecting samples in high water concentration often results in poor peak shapes. COSMOSIL PBr enables separation of hydrophilic compounds in reversed phase conditions, maintaining sharp peak shapes even with aqueous samples.

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: HILIC; Acetonitrile : Water = 90 : 10
PBr; Water
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 260 nm
Sample: 1. Uracil (0.1 mg/ml)
2. Uridine (0.2 mg/ml)
Sample solution: Water

Comparison with C18 Columns

COSMOSIL PBr retains hydrophilic compounds stronger than C18 columns under the same reversed-phase conditions.

Column size: 4.6 mmI.D. x 250 mm
Mobile phase: 20 mmol/l Phosphate Buffer (pH7.0)
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 225 nm
Sample: 1. Ammelide (0.1 mg/ml)
2. Cyanuric Acid (0.5 mg/ml)
3. Ammeline (0.1 mg/ml)
4. Malamine (0.1 mg/ml)
Injection vol.: 1.0 µl

Separation Mechanism

In reversed-phase chromatography, compounds are separated by difference in hydrophobicity (the number of CH2 bases, see chromatogram on the left (1)). Compounds with little hydrophobicity (the number of OCH2CH2, see chromatogram on the right (2)), are not retained by a C18 column. However, these compounds can easily be separated by dispersion force interaction using the COSMOSIL PBr column.

Compounds with more hydrophobicity

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Methanol : Water = 80 : 20
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 254 nm
Sample: 1. Benezene
2. Toluene
3. Ethylbenzene
4. n-Propylbenzene
5. n-Butylbenzene

Compounds with less hydrophobicity

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Methanol : Water = 60 : 40
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 254 nm
Sample: 1. Benzyl Alcohol
2. Ethylene Glycol Monobenzyl Ether
3. Diethylene Glycol Monobenzyl Ether
4. Triethylene Glycol Monobenzyl Ether
5. Tetraethylene Glycol Monobenzyl Ether

Dispersion force (instantaneous dipole-induced dipole force)

London Dispersion force is a weak intermolecular force that results from dipoles temporarily induced from random unsymmetrical electron positions in two adjacent atoms, also known as "instantaneous dipole-induced dipole force". It is present in all molecules regardless if they are polar or non-polar. Compounds with high polarizability have stronger dispersion force.

Compounds with stronger dispersion force

  • Larger and heavier molecules
  • Molecules with larger and heavier atoms (e.g. from weakest to strongest in halogens, F2, Cl2, Br2, and I2)
  • Molecules with delocalized electrons and resonance (e.g. aromatic compounds)

⇒ COSMOSIL PBr column is packed with pentabromobenzyl bonded silica that enables separation by dispersion force interaction.

Suitable Compounds to Analyze by COSMOSIL PBr

COSMOSIL PBr retains cyclic compounds or amide compounds more strongly than C18 columns.

(Mobile phase)
(1) Methanol : 20 mmol/l Phosphate buffer(pH7.0) = 10 : 90)
(2) 20 mmol/l Phosphate buffer (pH2.5))
(3) 20 mmol/l Phosphate buffer (pH7.0))
(4) Methanol : Water = 10 : 90

Column Stability Test

COSMOSIL PBr column remains stable after 1000 injections under 100% aqueous condition.

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: 20 mmol/l Phosphate buffer (pH2.5)
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 280 nm
Sample: 1. L-Noradrenaline (0.5 mg/ml)
2. L-Adrenaline (0.5 mg/ml)
3. Dopamine (0.5 mg/ml)
4. L-DOPA (0.5 mg/ml)
Injection vol.: 1.0 µl

Application

Gentamicin

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: 0.1% Pentafluoropropionic Acid –Acetonitrile : Water = 10 : 90
Flow rate: 0.2 ml/min
Temperature: 40°C
Detection: ESI-MS, Positive, SIM
Sample: Gentamicin
(C1a, C2, C2a, C1)
Injection vol.: 1.0 µl

Nucleic Acid Base

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Methanol : 20 mmol/l Phosphate buffer(pH7.0) = 10 : 90
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 260 nm
Sample: 1. Cytosine (0.05 mg/ml)
2. Uracil (0.05 mg/ml)
3. Thymine (0.05 mg/ml)
4. Guanine (0.05 mg/ml)
5. Adenine (0.05 mg/ml)
Injection vol.: 1.0 µl

Coenzyme A

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Methanol : 20 mmol/l Phosphate buffer(pH7.0) = 20 : 80
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 260 nm
Sample: 1. Malonyl Coenzyme A (0.25 mg/ml)
2. Coenzyme A (0.25 mg/ml)
3. Acetyl Coenzyme A (0.25 mg/ml)
Injection vol.: 2.0 µl

Methylimidazole Isomers

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: 20 mmol/l Phosphate buffer(pH2.5)
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 220 nm
Sample: 1. 1-Methylimidazole (0.1 mg/ml)
2. 2-Methylimidazole (0.1 mg/ml)
3. 4-Methylimidazole (0.1 mg/ml)
Injection vol.: 1.0 µl

Surfactants

COSMOSIL PBr shows excellent separation for surfactants compared to C18 or PFP columns.

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: 5C18-MS-II, 5PFP; Methanol : Water = 80 : 20
PBr; Methanol
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 254 nm
Sample: Triton® X-100 (50 mg/ml)
Injection vol.: 2.0 µl

Halogen Compounds

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Methanol : Water = 65 : 35
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV 254 nm
Sample: 1. Fluorobenzene (1.0 mg/ml)
2. α,α,α-Trifluorotoluene [Benzotrifluoride] (0.25 mg/ml)
3. Toluene (2.5 mg/ml)
4. Chlorobenzene (5.0 mg/ml)
5. Bromobenzene (5.0 mg/ml)
6. Iodobenzene (2.0 mg/ml)
Injection vol.: 1.0 µl

Downloads

Brochures
Posters
  • Evaluation of Pentabromobenzyl Stationary Phase for Separation of Polar Compounds by Reversed-Phase Chromatography
    (Poster from HPLC 2015, Geneva)
    pdf (370 KB)
  • Fat-Soluble and Water-Soluble Vitamins in Functional Water
    (Poster from Food Science Asia 2015, Singapore)
    pdf (870 KB)

Reference

  1. Malya, Irine Yunhafita, et al. "Plant growth regulators and Axl and immune checkpoint inhibitors from the edible mushroom Leucopaxillus giganteus." Bioscience, Biotechnology, and Biochemistry (2020): 1-7.
  2. Desmarchelier, Aurélien, Jonas Hamel, and Thierry Delatour. "Sources of overestimation in the analysis of acrylamide-in coffee by liquid chromatography mass spectrometry." Journal of Chromatography A 1610 (2020): 460566.
  3. Chen, Yue, et al. "Anti-inflammatory Limonoids From Cortex Dictamni." Frontiers in Chemistry 8 (2020): 73.
  4. Jiang, Dasen, et al. "Evaluation of a series of phenyl-type stationary phases in supercritical fluid chromatography with the linear solvation energy relationship model and its application to the separation of phenolic compounds." Journal of Chromatography A 1614 (2020): 460700.
  5. Segawa, Hiroki, et al. "Differentiation of ring‐substituted regioisomers of cathinone analogs by supercritical fluid chromatography." Analytical Science Advances.
  6. El-Shaheny, Rania, et al. "Pentabromobenzyl-RP versus triazole-HILIC columns for separation of the polar basic analytes famotidine and famotidone: LC method development combined with in silico tools to follow the potential consequences of famotidine gastric instability." Journal of Pharmaceutical and Biomedical Analysis (2020): 113305.
  7. Yoshii, Katsuhiro, et al. "Exploration of dipeptidyl-peptidase IV (DPP IV) inhibitors in a low-molecular mass extract of the earthworm Eisenia fetida and identification of the inhibitors as amino acids like methionine, leucine, histidine, and isoleucine." Enzyme and Microbial Technology 137 (2020): 109534.
  8. Delatour, Thierry, et al. "Thermal degradation of 2-furoic acid and furfuryl alcohol as pathways in the formation of furan and 2-methylfuran in food." Food chemistry 303 (2020): 125406.

Ordering Information

Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 3PBr Packed Column (2.0 mmI.D. x 50 mm ) 19345-91 Waters type 1PKG 568.00 Buy
COSMOSIL 3PBr Packed Column (2.0 mmI.D. x 75 mm ) 19346-81 Waters type 1PKG 568.00 Buy
COSMOSIL 3PBr Packed Column (2.0 mmI.D. x 100 mm ) 19347-71 Waters type 1PKG 568.00 Buy
COSMOSIL 3PBr Packed Column (2.0 mmI.D. x 150 mm ) 19078-01 Waters type 1PKG 568.00 Buy
COSMOSIL 3PBr Packed Column (2.0 mmI.D. x 250 mm ) 19348-61 Waters type 1PKG 678.00 Buy
COSMOSIL 3PBr Packed Column (3.0 mmI.D. x 50 mm ) 19349-51 Waters type 1PKG 568.00 Buy
COSMOSIL 3PBr Packed Column (3.0 mmI.D. x 75 mm ) 19350-11 Waters type 1PKG 568.00 Buy
COSMOSIL 3PBr Packed Column (3.0 mmI.D. x 100 mm ) 19351-01 Waters type 1PKG 568.00 Buy
COSMOSIL 3PBr Packed Column (3.0 mmI.D. x 150 mm ) 19352-91 Waters type 1PKG 568.00 Buy
COSMOSIL 3PBr Packed Column (3.0 mmI.D. x 250 mm ) 19353-81 Waters type 1PKG 678.00 Buy
COSMOSIL 3PBr Packed Column (4.6 mmI.D. x 50 mm ) 19354-71 Waters type 1PKG 568.00 Buy
COSMOSIL 3PBr Packed Column (4.6 mmI.D. x 75 mm ) 19355-61 Waters type 1PKG 568.00 Buy
COSMOSIL 3PBr Packed Column (4.6 mmI.D. x 100 mm) 19356-51 Waters type 1PKG 590.00 Buy
COSMOSIL 3PBr Packed Column (4.6 mmI.D. x 150 mm) 19357-41 Waters type 1PKG 590.00 Buy
COSMOSIL 3PBr Packed Column (4.6 mmI.D. x 250 mm) 19358-31 Waters type 1PKG 690.00 Buy
COSMOSIL 5PBr Packed Column (1.0 mmI.D. x 150 mm) 12881-71 Waters type 1PKG 475.00 Buy
COSMOSIL 5PBr Packed Column (2.0 mmI.D. x 50 mm) 12943-61 Waters type 1PKG 535.00 Buy
COSMOSIL 5PBr Packed Column (2.0 mmI.D. x 100 mm) 13245-81 Waters type 1PKG 505.00 Buy
COSMOSIL 5PBr Packed Column (2.0 mmI.D. x 150 mm) 12392-81 Waters type 1PKG 475.00 Buy
COSMOSIL 5PBr Packed Column (2.0 mmI.D. x 250 mm) 13247-61 Waters type 1PKG 515.00 Buy
COSMOSIL 5PBr Packed Column (3.0 mmI.D. x 50 mm) 12592-61 Waters type 1PKG 535.00 Buy
COSMOSIL 5PBr Packed Column (3.0 mmI.D. x 100 mm) 13249-41 Waters type 1PKG 505.00 Buy
COSMOSIL 5PBr Packed Column (3.0 mmI.D. x 150 mm) 13250-01 Waters type 1PKG 475.00 Buy
COSMOSIL 5PBr Packed Column (3.0 mmI.D. x 250 mm) 13251-91 Waters type 1PKG 515.00 Buy
COSMOSIL 5PBr Packed Column (4.6 mmI.D. x 50 mm) 13252-81 Waters type 1PKG 535.00 Buy
COSMOSIL 5PBr Packed Column (4.6 mmI.D. x 100 mm) 12594-41 Waters type 1PKG 490.00 Buy
COSMOSIL 5PBr Packed Column (4.6 mmI.D. x 150 mm) 12394-61 Waters type 1PKG 490.00 Buy
COSMOSIL 5PBr Packed Column (4.6 mmI.D. x 250 mm) 12395-51 Waters type 1PKG 515.00 Buy
COSMOSIL 5PBr Packed Column (10.0 mmI.D. x 50 mm) 13253-71 Waters type 1PKG 810.00 Buy
COSMOSIL 5PBr Packed Column (10.0 mmI.D. x 100 mm) 13254-61 Waters type 1PKG 940.00 Buy
COSMOSIL 5PBr Packed Column (10.0 mmI.D. x 150 mm) 13255-51 Waters type 1PKG 1,000.00 Buy
COSMOSIL 5PBr Packed Column (10.0 mmI.D. x 250 mm) 12397-31 Waters type 1PKG 1,575.00 Buy
COSMOSIL 5PBr Packed Column (20.0 mmI.D. x 100 mm) 13258-21 Waters type 1PKG 1,905.00 Buy
COSMOSIL 5PBr Packed Column (20.0 mmI.D. x 150 mm) 13259-11 Waters type 1PKG 2,005.00 Buy
COSMOSIL 5PBr Packed Column (20.0 mmI.D. x 250 mm) 12398-21 Waters type 1PKG 3,675.00 Buy
COSMOSIL 5PBr Packed Column (28.0 mmI.D. x 100 mm) 13260-71 Waters type 1PKG 5,350.00 Buy
COSMOSIL 5PBr Packed Column (28.0 mmI.D. x 150 mm) 13261-61 Waters type 1PKG 5,630.00 Buy
COSMOSIL 5PBr Packed Column (28.0 mmI.D. x 250 mm) 13262-51 Waters type 1PKG 7,550.00 Buy
Guard Column / Guard Cartridge
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 3PBr Guard Cartridge (2.0 mmI.D. x 10 mm ) 19359-34 Waters type 2PKG 202.00 Buy
COSMOSIL 3PBr Guard Cartridge (4.6 mmI.D. x 10 mm ) 19360-94 Waters type 2PKG 202.00 Buy
COSMOSIL 5PBr Guard Column (2.0 mmI.D. x 10 mm) 13287-41 Waters type 1PKG 450.00 Buy
COSMOSIL 5PBr Guard Cartridge (2.0 mmI.D. x 10 mm) 18196-94 Waters type 2PKG 230.00 Buy
COSMOSIL 5PBr Guard Cartridge (4.6 mmI.D. x 10 mm) 12444-14 Waters type 2PKG 230.00 Buy
COSMOSIL 5PBr Guard Column (20.0 mmI.D. x 20 mm) 13256-41 Waters type 1PKG 1,200.00 Buy
COSMOSIL 5PBr Guard Column (10.0 mmI.D. x 20 mm) 12396-41 Waters type 1PKG 550.00 Buy
COSMOSIL 5PBr Guard Column (28.0 mmI.D. x 50 mm) 12653-61 Waters type 1PKG 3,455.00 Buy
HPLC Accessories
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) 38009-79 Waters type 1 pkg 265.00 Buy
COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) 11884-71 Waters type 1 pkg 265.00 Buy
COSMOSIL Column Prefilter 39361-19 Waters type 1 pkg 145.00 Buy
COSMOSIL Column Spare Filter for Prefilter (1 micron) 39539-09 Waters type 2 pkg 55.00 Buy
COSMOSIL Column Connecting Tube (0.25 mm) 37843-69 Waters type 1 pkg 65.00 Buy
COSMOSIL Column Connecting Tube (0.1 mm) 12570-41 Waters type 1 pkg 65.00 Buy