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COSMOSIL PYE and COSMOSIL NPE

Specialty for Structual Isomers / π-π interaction, charge transfer and stereo selectivity

COSMOSIL PYE (pyrenylethyl group bonded) and COSMOSIL NPE (nitrophenylethyl group bonded) column show unique retention characteristics based on multiple separation modes such as hydrophobic, charge transfer and π-π interactions. These columns are recommended for the separation of structural isomers.

 COSMOSIL 5PYECOSMOSIL 5NPE
Silica gel high purity spherical porous silica
Average particle size 5µm
Average pore size approx. 120Å
Specific surface area approx. 300m²/g
Stationary phase 2-(1-pyrenyl)ethyl group nitrophenylethyl group
End capping treatment near-perfect treatment

 

Separation of PAHs

Due to the planar pyrene ring structure and strong π-π interactions, COSMOSIL PYE achieves excellent separation of aromatic isomers.

Advantage of dipole-dipole interactions

COSMOSIL NPE retains 1, 8-dinitronaphthalene longer than 1, 5-dinitronaphthalene due to the dipole-dipole and π-π interactions.

Usage Information

COSMOSIL PYE
Column5 µm PYE
I.D. (mm)2.03.04.610.020.0
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range pH2~pH7.5
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.5 µm or finer filter before use.
- As acetonitrile interferes with π electron interactions, please use a methanol-based mobile phase.
- The baseline may become unstable due to the high UV absorption of shedded stationary phase. If this occurs, please wash the column with THF. A small amount of stationary phase shedding will not affect performance.
- This column is not suitable for gradient analysis.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

COSMOSIL NPE

Column5 µm NPE
I.D. (mm)2.03.04.610.020.0
Washing method 5 µm C18-MS-II
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range pH2~pH7.5
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.5 µm or finer filter before use.
- As acetonitrile interferes with π electron interactions, please use a methanol-based mobile phase.
- The baseline may become unstable due to the high UV absorption of shedded stationary phase. If this occurs, please wash the column with THF. A small amount of stationary phase shedding will not affect performance.
- This column is not suitable for gradient analysis.

Downloads

Brochures
Posters
  • Evaluation of Pentabromobenzyl Stationary Phase for Separation of Polar Compounds by Reversed-Phase Chromatography
    (Poster from HPLC 2015, Geneva)
    pdf (370 KB)

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size

Ordering Information

COSMOSIL 5NPE
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 5NPE Packed Column (1.0 mm I.D. x 150 mm) 05897-01 Waters type 1 pkg 1,000.00 Buy
COSMOSIL 5NPE Packed Column (2.0 mm I.D. x 100 mm) 34500-71 Waters type 1 pkg 840.00 Buy
COSMOSIL 5NPE Packed Column (2.0 mm I.D. x 150 mm) 34328-51 Waters type 1 pkg 895.00 Buy
COSMOSIL 5NPE Packed Column (2.0 mm I.D. x 250 mm) 34379-91 Waters type 1 pkg 1,270.00 Buy
COSMOSIL 5NPE Packed Column (4.6 mm I.D. x 100 mm) 04673-51 Waters type 1 pkg 940.00 Buy
COSMOSIL 5NPE Packed Column (4.6 mm I.D. x 150 mm) 37902-21 Waters type 1 pkg 895.00 Buy
COSMOSIL 5NPE Packed Column (4.6 mm I.D. x 250 mm) 37990-71 Waters type 1 pkg 1,260.00 Buy
COSMOSIL 5NPE Packed Column (10.0 mm I.D. x 250 mm) 05469-11 Waters type 1 pkg 3,760.00 Buy
COSMOSIL 5NPE Packed Column (20.0 mm I.D. x 150 mm) 02638-81 Waters type 1 pkg 10,940.00 Buy
COSMOSIL 5NPE Packed Column (20.0 mm I.D. x 250 mm) 38046-21 Waters type 1 pkg 12,735.00 Buy
COSMOSIL 5NPE Packed Column (28.0 mm I.D. x 250 mm) 09553-71 Waters type 1 pkg 29,950.00 Buy
COSMOSIL 5NPE Guard Column (4.6 mm I.D. x 10 mm) 37904-01 Waters type 1 pkg 420.00 Buy
COSMOSIL 5NPE Guard Column (10.0 mm I.D. x 20 mm) 38045-31 Waters type 1 pkg 840.00 Buy
COSMOSIL 5NPE Guard Column (20.0 mm I.D. x 20 mm) 05868-81 Waters type 1 pkg 3,110.00 Buy
COSMOSIL 5NPE Guard Column (20.0 mm I.D. x 50 mm) 05869-71 Waters type 1 pkg 3,550.00 Buy
COSMOSIL 5PYE
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 5PYE Packed Column (1.0 mm I.D. x 50 mm) 04681-41 Waters type 1 pkg 1,155.00 Buy
COSMOSIL 5PYE Packed Column (1.0 mm I.D. x 150 mm) 02851-71 Waters type 1 pkg 1,450.00 Buy
COSMOSIL 5PYE Packed Column (2.0 mm I.D. x 50 mm) 18864-21 Waters type 1 pkg 1,250.00 Buy
COSMOSIL 5PYE Packed Column (2.0 mm I.D. x 100 mm) 34501-61 Waters type 1 pkg 1,115.00 Buy
COSMOSIL 5PYE Packed Column (2.0 mm I.D. x 150 mm) 38042-61 Waters type 1 pkg 1,450.00 Buy
COSMOSIL 5PYE Packed Column (2.0 mm I.D. x 250 mm) 34450-31 Waters type 1 pkg 1,955.00 Buy
COSMOSIL 5PYE Packed Column (4.6 mm I.D. x 50 mm) 38043-51 Waters type 1 pkg 985.00 Buy
COSMOSIL 5PYE Packed Column (4.6 mm I.D. x 100 mm) 04672-61 Waters type 1 pkg 1,260.00 Buy
COSMOSIL 5PYE Packed Column (4.6 mm I.D. x 150 mm) 37837-91 Waters type 1 pkg 1,345.00 Buy
COSMOSIL 5PYE Packed Column (4.6 mm I.D. x 250 mm) 37989-11 Waters type 1 pkg 1,965.00 Buy
COSMOSIL 5PYE Packed Column (10.0 mm I.D. x 250 mm) 37996-11 Waters type 1 pkg 6,155.00 Buy
COSMOSIL 5PYE Packed Column (20.0 mm I.D. x 150 mm) 05948-31 Waters type 1 pkg 17,335.00 Buy
COSMOSIL 5PYE Packed Column (20.0 mm I.D. x 250 mm) 38044-41 Waters type 1 pkg 23,260.00 Buy
COSMOSIL 5PYE Packed Column (28.0 mm I.D. x 250 mm) 34300-91 Waters type 1 pkg 49,200.00 Buy
COSMOSIL 5PYE Guard Column (2.0 mm I.D. x 10 mm) 17787-71 Waters type 1 pkg N/A Inquire
COSMOSIL 5PYE Guard Column (4.6 mm I.D. x 10 mm) 37903-11 Waters type 1 pkg 450.00 Buy
COSMOSIL 5PYE Guard Column (10.0 mm I.D. x 20 mm) 38041-71 Waters type 1 pkg 1,430.00 Buy
COSMOSIL 5PYE Guard Column (20.0 mm I.D. x 20 mm) 05867-91 Waters type 1 pkg 4,860.00 Buy
COSMOSIL 5PYE Guard Column (20.0 mm I.D. x 50 mm) 34475-21 Waters type 1 pkg 6,070.00 Buy