Easy to Use: No need for pretreatment, washing, destaining or microwaving.
Safe Use: No harmful methanol or acetic acid is contained.
High Sensitivity: As little as several tens of ng protein (BSA) can be detected.
Mass Spec Compatible
Protocol for staining mini-gel
Gently mix CosmoPAGE Bullet CBB right before use by inverting a couple of times.
Immerse the gel in sufficient volume of CosmoPAGE Bullet CBB (~20 ml) to cover the gel. Washing before staining is NOT required. Transfer the gel directly into the staining solution.
Incubate for 15 minutes at room temperature with gentle shaking. Destaining is NOT required.
The stained gel can be stored in the staining solution. Alternatively, the stained gel can be stored in water after staining for 1 hour with CosmoPAGE Bullet CBB.
Expiration
2 years. Expiration date is stated on the product label (Exp. yy/mm)
Note
When the stained gel is stored in the staining solution in refrigerator, precipitation may occur. Simply wash the stained gel with water for 30 minutes to 1 hour if necessary.
The stain intensity may decrease if the gel is left in water for a long time.
For the gel drying or the complete background destaining is desired, make sure the gel has been stained for 1 hour, and incubate the gel in water for at least 1 hour.
Wear protective gloves while handling Bullet CBB Stain One.
Bullet CBB Stain One is acidic dye-solution. Discard it according to the regulation in your region.