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Recombinant Human Activin A, Animal-free

Introducing animal-free, recombinant Human Activin A expressed and purified using an Escherichia coli expression system, and manufactured by Ajinomoto Co. (Japan). Activin A is a member of the transforming growth factor β (TGF-β) family, and is used for the proliferation of pluripotent stem cells, as well as differentiation into endoderm and other different lineages.
This product is for research use only.

  • Free from components of animal origin
  • Frozen solution, easy to use
  • High purity and activity
  • High batch homogeneity

Comparison of product specification

Non-GMP product

  AJINOMOTO Company ACompany BCompany C
Host cell E. coli E. coli CHO Human
Endotoxin <0.01EU/μg
(LAL)
<1EU/μg
(LAL)
<0.01EU/μg
(LAL)
<0.01EU/μg
(LAL)
Purity ≧97%
(SDS-PAGE)
≧97%
(SDS-PAGE、HPLC)
>95%
(SDS-PAGE)
>95%
(SDS-PAGE)
Biological
activity*1
ED50, 50-150% vs.
WHO std*2
(K562)
ED50, ≦2.0 ng/ml
(MPC-11)
ED50, 0.2-1.2 ng/ml
(K562)
ED50, 0.5-5 ng/ml
(MPC-11)
Form Frozen
(0.1 mg/ml)
Lyophilized Lyophilized Lyophilized
N-terminal Gly-Leu-Glu Gly
Expiration date 4 years from
manufacturing date
1 year from
date of receipt
1 year from
date of receipt
  • *1 Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cell.
  • *2 Activin A WHO international std., NIBSC code: 91/626

 

GMP compliant product

  AJINOMOTO Company ACompany BCompany D
Host cell E. coli E. coli CHO E. coli
Endotoxin <0.01EU/μg
(LAL)
≦0.10EU/μg
(LAL)
<0.10EU/μg
(LAL)
<0.05EU/μg
(LAL)
Purity ≧97%
(SDS-PAGE)
≧98%
(SDS-PAGE、HPLC)
>97%
(SDS-PAGE)
≧97%
(SDS-PAGE)
*include ≦5% oligomers
Biological
activity*1
ED50, 50-150% vs.
WHO std*2
(K562)
ED50, 1.0-5.0 ng/ml
(MPC-11)
ED50, 0.2-1.2 ng/ml
(K562)
0.6–2.5x103 IU/mg
(MPC-11)
Mycoplasma Negative
(NAT)
Negative
(QF-PCR)
Negative
(Ribosomal RNA
hybridization assay)
Sterility Conform
(Membrane filter)
No growth
(Direct inoculation)
No growth
(Direct inoculation)
Form Frozen
(0.1 mg/ml)
Lyophilized Lyophilized Lyophilized
N-terminal Gly-Leu-Glu Gly-Leu-Glu Gly-Leu-Glu Met-Gly-Leu-Glu
Expiration date 4 years from
manufacturing date
5 years from
manufacturing date
3 years from
bottling date
Minimum 6 months
from shipping date
  • *1 Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cell.
  • *2 Activin A WHO international std., NIBSC code: 91/626

Comparison of biological activity with other companies’ GMP products

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cell.
Activin A WHO international std., NIBSC code: 91/626

AJINOMOTO’s Activin A demonstrates equal to superior biological activity compared to tested alternatives!

Comparison of MS spectrum with other companies’ GMP products

AJINOMOTO

 

Company X

 

Company Y

 

AJINOMOTO’s Activin A also contains significantly less impurities!

Stability information after reconstitution

  AJINOMOTO Company A Company B
-80°C 4 years
from manufacturing date
At least 7 months3 months
4°C At least 6 months 1 week 1 month
Freeze-thaw cycles Within 2 times
(>90% activity)

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Recombinant Human Activin A activin-10 -20°C 10 µg 185.00 Buy
Recombinant Human Activin A activin-50 -20°C 50 µg 775.00 Buy
Recombinant Human Activin A activin-1000 -20°C 1 mg 5,835.00 Buy
Recombinant Human Activin A (GMP grade) activin-GMP -20°C 1 mg N/A Inquire