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mRNA and Oligonucleotides purification with chromatography

RNA based therapeutics represent a powerful new tool for gene therapy. In RNA production, impurities such as nucleotide triphosphates, abortive transcripts, and DNA template can affect down-stream applications by triggering immune responses. Gel-based methods of purification, such as preparative denaturing polyacrylamide gel electrophoresis (PAGE) and agarose gels, are limited by poor resolution of RNA size and potential chemical modifications from reagents such as formaldehyde.

To date, high performance liquid chromatography (HPLC) remains a staple method for purification of messenger RNA (mRNA) and RNA oligonucleotides. Presented here are two HPLC methods, a size exclusion-based method for the purification of long mRNA, and a reverse-phase method for the purification of oligonucleotides.

HPLC Purification of mRNA with Size Exclusion Chromatography

Figure 1. mRNA application by RNA-SEC-100.

 

Analyze and purify mRNA up to 5,000 bases !

RNA-SEC-2000 Application

Figure 2. Molecular weight determination for mRNA up to 5,000 bases

 

Analysis of Nucleotides by Reverse-Phase Chromatography

Application of ΦX174 HaeIII digest fragments using wide pore C18 column

 >> Information on COSMOSIL RNA-RP1 (under construction)

 

Single base resolution at 40 nucleotides

>> Information on COSMOCORE 2.6C18

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size

Ordering Information

COSMOCORE 2.6C18
Product Cat.No. PKG Size Price(US$)  
COSMOCORE 2.6C18 packed column (2.1 mm I.D. x 100 mm) 12614-71 1 pkg 760.00 Buy
COSMOSIL RNA-RP1
Product Cat.No. PKG Size Price(US$)  
COSMOSIL RNA-RP1 Packed Column (2.0 mm I.D. x 100 mm) 21078-31 1 pkg 1,480.00 Buy
COSMOSIL RNA-RP1 Packed Column (4.6 mm I.D. x 100 mm) 21079-21 1 pkg 1,480.00 Buy
COSMOSIL RNA-RP1 Packed Column (10.0 mm I.D. x 100 mm) 21080-81 1 pkg 3,480.00 Buy
COSMOSIL RNA-SEC-1000
Product Cat.No. PKG Size Price(US$)  
COSMOSIL RNA-SEC-1000 Packed Column (4.6 mm I.D. x 250 mm) 21088-01 1 pkg 1,980.00 Buy
COSMOSIL RNA-SEC-1000 Guard Column (7.5 mm I.D. x 50 mm) 20785-91 1 pkg 950.00 Buy
COSMOSIL RNA-SEC-1000 Packed Column (7.5 mm I.D. x 300 mm) 19380-21 1 pkg 2,800.00 Buy
COSMOSIL RNA-SEC-2000
Product Cat.No. PKG Size Price(US$)  
COSMOSIL RNA-SEC-2000 Packed Column (4.6 mm I.D. x 250 mm) 21095-01 1 pkg 1,980.00 Buy
COSMOSIL RNA-SEC-2000 Guard Column (7.5 mm I.D. x 50 mm) 21096-91 1 pkg 950.00 Buy
COSMOSIL RNA-SEC-2000 Packed Column (7.5 mm I.D. x 300 mm) 19381-11 1 pkg 2,800.00 Buy