mRNA and Oligonucleotides purification with chromatography
RNA based therapeutics represent a powerful new tool for gene therapy. In RNA production, impurities such as nucleotide triphosphates, abortive transcripts, and DNA template can affect down-stream applications by triggering immune responses. Gel-based methods of purification, such as preparative denaturing polyacrylamide gel electrophoresis (PAGE) and agarose gels, are limited by poor resolution of RNA size and potential chemical modifications from reagents such as formaldehyde.
To date, high performance liquid chromatography (HPLC) remains a staple method for purification of messenger RNA (mRNA) and RNA oligonucleotides. Presented here are two HPLC methods, a size exclusion-based method for the purification of long mRNA, and a reverse-phase method for the purification of oligonucleotides.
HPLC Purification of mRNA with Size Exclusion Chromatography
Figure 1. mRNA application by RNA-SEC-100.
Analyze and purify mRNA up to 5,000 bases !
Figure 2. Molecular weight determination for mRNA up to 5,000 bases
Analysis of Nucleotides by Reverse-Phase Chromatography
Application of ΦX174 HaeIII digest fragments using wide pore C18 column
>> Information on COSMOSIL RNA-RP1 (under construction）
Single base resolution at 40 nucleotides
Application Data
COSMOSIL RNA-RP1 Application Data (12.15.2021)COSMOSIL Application Search
COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size
Ordering Information
COSMOCORE 2.6C18
| Product | Cat.No. | PKG Size | Price(US$) | |
|---|---|---|---|---|
| COSMOCORE 2.6C18 packed column (2.1 mm I.D. x 100 mm) | 12614-71 | 1 pkg | 760.00 | Buy |
COSMOSIL RNA-RP1
| Product | Cat.No. | PKG Size | Price(US$) | |
|---|---|---|---|---|
| COSMOSIL RNA-RP1 Packed Column (2.0 mm I.D. x 100 mm) | 21078-31 | 1 pkg | 1,480.00 | Buy |
| COSMOSIL RNA-RP1 Packed Column (4.6 mm I.D. x 100 mm) | 21079-21 | 1 pkg | 1,480.00 | Buy |
| COSMOSIL RNA-RP1 Packed Column (10.0 mm I.D. x 100 mm) | 21080-81 | 1 pkg | 3,480.00 | Buy |
COSMOSIL RNA-SEC-1000
| Product | Cat.No. | PKG Size | Price(US$) | |
|---|---|---|---|---|
| COSMOSIL RNA-SEC-1000 Packed Column (4.6 mm I.D. x 250 mm) | 21088-01 | 1 pkg | 1,980.00 | Buy |
| COSMOSIL RNA-SEC-1000 Guard Column (7.5 mm I.D. x 50 mm) | 20785-91 | 1 pkg | 950.00 | Buy |
| COSMOSIL RNA-SEC-1000 Packed Column (7.5 mm I.D. x 300 mm) | 19380-21 | 1 pkg | 2,800.00 | Buy |
COSMOSIL RNA-SEC-2000
| Product | Cat.No. | PKG Size | Price(US$) | |
|---|---|---|---|---|
| COSMOSIL RNA-SEC-2000 Packed Column (4.6 mm I.D. x 250 mm) | 21095-01 | 1 pkg | 1,980.00 | Buy |
| COSMOSIL RNA-SEC-2000 Guard Column (7.5 mm I.D. x 50 mm) | 21096-91 | 1 pkg | 950.00 | Buy |
| COSMOSIL RNA-SEC-2000 Packed Column (7.5 mm I.D. x 300 mm) | 19381-11 | 1 pkg | 2,800.00 | Buy |