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COSMOSIL HILIC

Others / Hydrophilic interaction

COSMOSIL HILIC is a new column for hydrophilic interaction chromatography with Triazole bonded silica packing material. The hydrophilic interaction chromatography is a variation of normal phase chromatography where a polar stationary phase is used with a mobile phase which contains a high concentration of organic solvent and a low concentration of aqueous eluent. The main retention mechanism is the partitioning of the polar analytes between the polar stationary and the non-polar mobile phase. As it is also called "aqueous normal phase", the elution order is similar to that of normal phase and the sample elution is in the order of increasing hydrophilicity. Without using ion-pair reagent COSMOSIL HILIC retains highly polar analytes that would not be retained in reversed phase chromatography. It also shows a weak anion-exchange mechanism with the positively charged stationary phase, thus acidic compound is strongly retained.

COSMOSIL HILIC
USP code L104
Silica gel High purity porous spherical silica gel
Average particle size 2.5 µm 5 µm
Average pore size approx. 130Å approx. 120Á
Specific surface area approx. 330m²/g approx. 300m²/g
Stationary phase Triazole
Main interaction Hydrophilic interaction

Comparison with C18 column

COSMOSIL HILIC can separate Glycine and Glycylglycine without ion-pair reagent. Although C18 column can separate them with ion-pair reagents, there are some disadvantages such as column equilibration, preparation of mobile phase and column deterioration.

 

Different Interactions

Separation of Anionic Compounds

Anionic compounds were used to evaluate the anion-exchange capability. The only COSMOSIL HILIC showed strong selectivity of anionic compounds.

 

Separation by Hydrophilic Interaction

The retention mechanism of COSMOSIL HILIC is the combination of hydrophilic interaction and anion-exchange, and the retention can be controlled by changing the mobile phase.

 

Separation of Acidic, Basic and Neutral Compounds

Acidic (Glyceric Acid), basic (Tris) and neutral (meso-Erythritol) compounds were used for evaluation of anion and cationexchange characteristics. The separation factor a(Acid/Neutral) indicates its anion-exchange capability and the factor a(Basic/ Neutral) shows its cation-exchange effect.

Analysis of Taurine and Hypotaurine by HILIC

Taurine is a biologically significant molecule with a zwitterionic character, which makes it a difficult analysis target. COSMOSIL HILIC has a unique anion-exchange mechanism which allows strong retention of taurine with good peak shape. It is USP packing type L104, specified on the official monogram for taurine.

>> USP revision notice for taurine (PDF file)

 

Application Data

▶  Melamine analysis by HPLC and LC/MS/MS (PDF)

 
   
Melamine Allantoins
   
BCAA (Amino Acid Branched-chain) Juice Components
   
Phosphorylated Saccharides Water-soluble Vitamins
   
Hydrophilic Drugs Natural Products

Combination with C18 columns

Selection of Mobile Phase

Following are the recommended mobile phases for different samples.

- Neutral Compounds Acetonitrile / Water = 90 / 10
- Basic Compounds Acetonitrile / 10mmol/l CH3COONH4 = 90 / 10
- Amphoteric Compounds Acetonitrile / 10mmol/l CH3COONH4 = 70 / 30
- Acidic Compounds Acetonitrile / 10mmol/l CH3COONH4 = 50 / 50
  (not eluted) → Acetonitrile / 10mmol/l Phosphatase buffer (pH7.0) = 50 / 50

COSMOSIL HILIC Application Notebook

COSMOSIL HILIC Application Notebook contains about 200 chromatograms for the separation of polar compounds using COSMOSIL HILIC column. It also describes how the mobile phase conditions, such as buffer pH and salt concentration influence the separation in HILIC mode.

COSMOSIL HILIC Application Notebook
COSMOSIL HILIC Application Notebook (PDF) : 18.5 MB

Reference

Development of a simple method for quantitation of methanesulfonic acid at low ppm level using hydrophilic interaction chromatography coupled with ESI-MS
Zongyun Huang, Robert Francis, Yan Zha, Joan Ruan
Journal of Pharmaceutical and Biomedical Analysis, 102, 2015, 17-24
 
Grevillosides J-Q, Arbutin Derivatives from the Leaves of Grevillea robusta and Their Melanogenesis Inhibitory Activity
Yukiko Yamashita-Higuchi, Sachiko Sugimoto, Katsuyoshi Matsunami, Hideaki Otsuka, Takahito Nakai
Chem. Pharm. Bull., 62 (4), 2014, 364-372
 
Tetrodotoxin and paralytic shellfish poisons in gastropod species from Vietnam analyzed by high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry
Hsiao-Chin Jen, Thi Anh-Tuyet Nguyen, Ya-Jung Wu, Tung Hoang, Osamu Arakawa, Wen-Feng Lin, Deng-Fwu Hwang
Journal of Food and Drug Analysis, 22 (2), 2014, 178-188
 
Hyrtimomines, indole alkaloids from Okinawan marine sponges Hyrtios spp.
Naonobu Tanaka, Rei Momose, Azusa Takahashi-Nakaguchi, Tohru Gonoi, Jane Fromont, Jun'ichi Kobayashi
Tetrahedron, 70 (4), 2014, 832-837
 
Bromopyrrole Alkaloids from a Marine Sponge Agelas sp.
Taishi Kusama, Naonobu Tanaka, Azusa Takahashi-Nakaguchi, Tohru Gonoi, Jane Fromont, Jun’ichi Kobayashi
Chem. Pharm. Bull., 62 (5), 2014, 499-503
 
Recent advances in hydrophilic interaction chromatography for quantitative analysis of endogenous and pharmaceutical compounds in plasma samples
Muneki Isokawa, Takahiro Kanamori, Takashi Funatsu-- & Makoto Tsunoda--
Bioanalysis, 6 (18), 2014, 2421-2439
 
Synthesis of stationary phases containing pyridine, phenol, aniline and morpholine via click chemistry and their characterization and evaluation in supercritical fluid chromatography
Melissa Dunkle, Caroline West, Alberto Pereira, Steven Van der Plas, Annemieke Madder, William Farrell, Eric Lesellier, Frederic Lynen, Pat Sandra
Scientia Chromatographica, 6 (2), 2014, 85-103
 
Comparative assessment of achiral stationary phases for high throughput analysis in supercritical fluid chromatography
Katalin Ebinger, Harold N. Weller
Journal of Chromatography A, 1332 (7), 2014, 73-81
 
Effect of oxygen reduction during malaxation on the quality of extra virgin olive oil (Cv. Carboncella) extracted through “two-phase” and “three-phase” centrifugal decanters
Gianni Pastore, Antonio D'Aloise, Sabrina Lucchetti, Mariateresa Maldini, Elisabetta Moneta, Marina Peparaio, Antonio Raffo, Fiorella Sinesio
LWT - Food Science and Technology, 59 (1), 2014, 163-172
 
Unusual amino acid derivatives from the mushroom Pleurocybella porrigens
Takumi Kawaguchi, Tomohiro Suzuki, Yuka Kobayashi, Shinya Kodani, Hirofumi Hirai, Kaoru Nagai and Hirokazu Kawagishi
Tetrahedron, 66(2), 2010, 504-507

Ordering Information

Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOSIL 2.5HILIC Packed Column (2.0 mmI.D. x 50 mm) 11766-21 Waters type 1 pkg 745.00 Buy
COSMOSIL 2.5HILIC Packed Column (2.0 mmI.D. x 75 mm) 11768-01 Waters type 1 pkg 745.00 Buy
COSMOSIL 2.5HILIC Packed Column (2.0 mmI.D. x 100 mm) 11769-91 Waters type 1 pkg 745.00 Buy
COSMOSIL 2.5HILIC Packed Column (2.0 mmI.D. x 150 mm) 11770-51 Waters type 1 pkg 745.00 Buy
COSMOSIL 2.5HILIC Packed Column (3.0 mmI.D. x 50 mm) 11771-41 Waters type 1 pkg 745.00 Buy
COSMOSIL 2.5HILIC Packed Column (3.0 mmI.D. x 75 mm) 11772-31 Waters type 1 pkg 745.00 Buy
COSMOSIL 2.5HILIC Packed Column (3.0 mmI.D. x 100 mm) 11773-21 Waters type 1 pkg 745.00 Buy
COSMOSIL 2.5HILIC Packed Column (3.0 mmI.D. x 150 mm) 11774-11 Waters type 1 pkg 745.00 Buy
COSMOSIL 5HILIC Packed Column (1.0 mml.D. x 150 mm) 07869-11 Waters type 1 pkg 440.00 Buy
COSMOSIL 5HILIC Packed Column (1.0 mml.D. x 250 mm) 07870-71 Waters type 1 pkg 515.00 Buy
COSMOSIL 5HILIC Packed Column (2.0 mml.D.x 30 mm) 08568-21 Waters type 1 pkg 440.00 Buy
COSMOSIL 5HILIC Packed Column (2.0 mml.D.x 50 mm) 07052-91 Waters type 1 pkg 525.00 Buy
COSMOSIL 5HILIC Packed Column (2.0 mml.D.x 100 mm) 08569-11 Waters type 1 pkg 440.00 Buy
COSMOSIL 5HILIC Packed Column (2.0 mml.D.x 150 mm) 07054-71 Waters type 1 pkg 440.00 Buy
COSMOSIL 5HILIC Packed Column (2.0 mml.D.x 250 mm) 07489-91 Waters type 1 pkg 515.00 Buy
COSMOSIL 5HILIC Packed Column (3.0 mml.D. x 150 mm) 07871-61 Waters type 1 pkg 475.00 Buy
COSMOSIL 5HILIC Packed Column (3.0 mml.D. x 250 mm) 07872-51 Waters type 1 pkg 515.00 Buy
COSMOSIL 5HILIC Packed Column (4.6 mml.D.x 50 mm) 07973-31 Waters type 1 pkg 460.00 Buy
COSMOSIL 5HILIC Packed Column (4.6 mml.D.x 150 mm) 07056-51 Waters type 1 pkg 440.00 Buy
COSMOSIL 5HILIC Packed Column (3 Lots Set) (4.6 mml.D.x 150 mm) 09385-23 Waters type 3 pkg 1,325.00 Buy
COSMOSIL 5HILIC Packed Column (4.6 mml.D.x 250 mm) 07057-41 Waters type 1 pkg 515.00 Buy
COSMOSIL 5HILIC Packed Column (10 mml.D.x 150 mm) 05564-51 Waters type 1 pkg 1,040.00 Buy
COSMOSIL 5HILIC Packed Column (10 mml.D.x 250 mm) 07059-21 Waters type 1 pkg 1,260.00 Buy
COSMOSIL 5HILIC Packed Column (20 mml.D.x 250 mm) 07060-81 Waters type 1 pkg 2,760.00 Buy
COSMOSIL 5HILIC Packed Column (28mml.D. x 250 mm) 07875-21 Waters type 1 pkg 5,260.00 Buy
Guard Column / Guard Cartridge
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOSIL 5HILIC Guard Cartridge (4.6 mm I.D. x 10 mm) 19184-14 Waters type 2 pkg 220.00 Buy
COSMOSIL 5HILIC Guard Column (2.0 mmI.D. x 10 mm) 07569-41 Waters type 1 pkg 370.00 Buy
COSMOSIL 5HILIC Guard Column (4.6 mml.D. x 10 mm) 07055-61 Waters type 1 pkg 265.00 Buy
COSMOSIL 5HILIC Guard Column (10.0 mml.D. x 20 mm) 07058-31 Waters type 1 pkg 460.00 Buy
COSMOSIL 5HILIC Guard Column (20.0 mml.D. x 20 mm) 07854-91 Waters type 1 pkg 1,000.00 Buy
COSMOSIL 5HILIC Guard Column (20.0 mml.D. x 50 mm) 07873-41 Waters type 1 pkg 1,375.00 Buy
COSMOSIL 5HILIC Guard Column (28.0 mml.D. x 50 mm) 07874-31 Waters type 1 pkg 3,035.00 Buy
HPLC Accessories
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) 38009-79 Waters type 1 pkg 265.00 Buy
COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) 11884-71 Waters type 1 pkg 265.00 Buy
COSMOSIL Column Prefilter 39361-19 Waters type 1 pkg 145.00 Buy
COSMOSIL Column Spare Filter for Prefilter (1 micron) 39539-09 Waters type 2 pkg 55.00 Buy
COSMOSIL Column Connecting Tube (0.25 mm) 37843-69 Waters type 1 pkg 65.00 Buy
COSMOSIL Column Connecting Tube (0.1 mm) 12570-41 Waters type 1 pkg 65.00 Buy

** Guard cartridges require a guard cartridge holder.