His-Detect In-Gel Stain
Staining His-tagged fusion proteins
His-Detect In-Gel Stain is a ready-to-use staining reagent for visualizing His-tagged fusion protein directly in a polyacrylamide gel.
Features
- Stain His-tagged fusion proteins directly in gels without western blotting
- Detect picomolar level of His-tagged fusion proteins within an hour
- No fluorescent image analyzer or transilluminator required
Application Data: Comparison with Western Blotting
Western blotting
| Primary antibody : | Anti-6xHis (Cat. No.: 04428) |
| Secondary antibody : | Anti-Mouse IgG (Goat), HRP-conjugate (SantaCruz, Cat. No.: SC-2005) |
| Chemiluminescence detection : | Chemi-Lumi One L (Cat. No.: 07880) |
| Colorimetric detection : | Peroxidase Stain Kit (Cat. No.: 26652) |
| Sample: |
Lane 1 Prestained Protein Markers (Broad Range) (Cat. No.: 02525)
Lane 2 Protein Markers (Biotinylated) (Cat. No.: 02886)
Lane 3-6 Dilution series (x1/1, x1/2, x1/3, x1/4, respectively) of DHFR-His expression in E.coli Lysate
|
* For detection of Protein Markers (Biotinylated), Streptavidin Horseradish Peroxidase Conjugate (Cat. No.: 02517) is used.
Procedure comparison
Using His-Detect In-Gel Stain vs. conventional western blotting
| His-Detect In-Gel Stain | Western Blotting | |
|
Number of steps
(except washing)
|
1 steps
|
5 steps
1. Transfer
2. Blocking
3. Primary Ab reaction
4. Secondary Ab reaction
5. Detection
|
| Time | 60 min. or less | 300 min. or more |
His-Detect In-Gel Stain (Brochure)