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His-Detect In-Gel Stain

Staining His-tagged fusion proteins

His-Detect In-Gel Stain is a ready-to-use staining reagent for visualizing His-tagged fusion protein directly in a polyacrylamide gel.

Features

- Stain His-tagged fusion proteins directly in gels without western blotting
- Detect picomolar level of His-tagged fusion proteins within an hour
- No fluorescent image analyzer or transilluminator required

Application Data: Comparison with Western Blotting

 

Western blotting

Primary antibody : Anti-6xHis (Cat. No.: 04428)
Secondary antibody : Anti-Mouse IgG (Goat), HRP-conjugate (SantaCruz, Cat. No.: SC-2005)
Chemiluminescence detection : Chemi-Lumi One L (Cat. No.: 07880)
Colorimetric detection : Peroxidase Stain Kit (Cat. No.: 26652)
Sample: 
Lane 1  Prestained Protein Markers (Broad Range) (Cat. No.: 02525)
Lane 2  Protein Markers (Biotinylated) (Cat. No.: 02886)
Lane 3-6   Dilution series (x1/1, x1/2, x1/3, x1/4, respectively) of DHFR-His expression in E.coli Lysate

  * For detection of Protein Markers (Biotinylated), Streptavidin Horseradish Peroxidase Conjugate (Cat. No.: 02517) is used.

Procedure comparison

Using His-Detect In-Gel Stain vs. conventional western blotting

  His-Detect In-Gel Stain Western Blotting
Number of steps
(except washing)
1 steps
5 steps
     1. Transfer
     2. Blocking
     3. Primary Ab reaction
     4. Secondary Ab reaction
     5. Detection
Time 60 min. or less 300 min. or more

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
His-Detect In-Gel Stain 08500-94 Room Temp. 100 ml 50.00 Buy
His-Detect In-Gel Stain 08500-65 Room Temp. 500 ml 180.00 Buy