| Product Name | Chemi-Lumi One L | Chemi-Lumi One Super | Chemi-Lumi One Ultra |
|---|---|---|---|
| Product No. | 07880-70 | 02230-30 | 11644-40 |
| Usage | General Use | High Sensitive | Ultra high Sensitive |
| Lower Detection Limit | Low-picogram | Mid-femtogram | Low-femtogram |
| Required Working Solution | approx. 0.125 ml / cm2 | approx. 0.1 ml / cm2 | approx. 0.1 ml / cm2 |
| Suggested Antibody Dilution | Primary: 1:1,000-1:5,000 Secondary: 1:20,000-1:100,000 |
Primary: 1:1,000-1:20,000 Secondary: 1:20,000-1:200,000 |
Primary: 1:5,000-1:100,000 Secondary: 1:100,000-1:500,000 |
| Reaction Period | 1 min. | 1 min. | 5 min. |
| Comparable to | Amersham TM ECLTM SuperSignal® West Pico® |
Amersham TM ECLTM Prime SuperSignal® West Dura® |
Amersham TM ECLTM Select SuperSignal® West Femto® |
| Sensitivity |
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Chemiluminescent HRP Substrates : Chemi-Lumi One L
Picogram Sensitivity Substrate for HRP / Economical - costs much less than other ECL substrate
Chemi-Lumi One is a series of high sensitive luminol-based chemiluminescence assay kits for Western Blotting. We offer three types of chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP).
Features
- Picogram sensitivity - better signal than Amersham TM ECLTM Substrate
- Economical - costs much less than other Amersham TM ECLTM Substrate
- Long light emission
Chemi-Lumi One Series for HRP
Chemi-Lumi One is a series of high sensitive luminol-based chemiluminescence assay kits for Western Blotting. We offer three types of chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP).
Comparison of sensitivity and duration
Chemi-Lumi One L has more sensitive and longer signal duration than GE ECL Substrate. Western blot of identical biotinylated samples probed with respective streptavidin-HRP conjugates. The membranes were incubated with substrate that was prepared according to the manufacturers' instructions.
| Lane1: 1: 50 dilution | Lane3: 1: 200 |
| Lane2: 1: 100 dilution | Lane4: 1: 400 |
Chemi-Lumi One L (Instruction)Reference
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Yamashita, Kaoru, et al. "Adrenal cortex hypoxia modulates aldosterone production in heart failure." Biochemical and Biophysical Research Communications (2020).
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Chihara, Yusuke, et al. "Histone deacetylase inhibitor OBP‑801 and amrubicin synergistically inhibit the growth of squamous cell lung carcinoma by inducing mitochondrial ASK1‑dependent apoptosis." International Journal of Oncology 56.3 (2020): 848-856.
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Sheikh, Amin Alborzian Deh, Chizuru Akatsu, and Takeshi Tsubata. "Identification of Siglec Cis-Ligands by Proximity Labeling." Lectin Purification and Analysis. Humana, New York, NY, 2020. 75-83.
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Roudkenar, Mehryar Habibi, et al. "Disturbance in the regulation of miR 17-92 cluster on HIF-1-α expression contributes to clinically relevant radioresistant cells: an in vitro study." Cytotechnology (2020): 1-13.
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Umekawa, Midori, et al. "Mitotic cyclin Clb4 is required for the intracellular adaptation to glucose starvation in Saccharomyces cerevisiae." FEBS letters 594.8 (2020): 1329-1338.
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Norikura, Toshio, et al. "cis-Banglene, a bangle (Zingiber purpureum)-derived bioactive compound, promotes mitochondrial biogenesis and glucose uptake by activating the IL-6/AMPK signaling pathway in C2C12 skeletal muscle cells." Journal of Functional Foods 64 (2020): 103632.
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Kasahara, Takaoki, et al. "Cardiolipin is essential for early embryonic viability and mitochondrial integrity of neurons in mammals." The FASEB Journal (2020).
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Uehara, Natsumi, et al. "Oncolytic effect of Midkine promoter–based conditionally replicating adenoviruses expressing EGFR siRNA in head and neck squamous cancer cell line T891." Cancer Reports.
Comparison of price
|
Company
|
Nacalai USA, Inc.
|
Cytiva
|
|---|---|---|
|
Item
|
Chemi-Lumi One L
|
Amersham ECL
Western Blotting Detection Reagent
|
|
Price
|
$200
|
$347
|
|
Cat. No.
|
07880-70
|
RPN2106
|
|
Quantity
|
for 4,000 cm² membrane
|
for 4,000 cm² membrane
|
Ordering Information
| Product | Storage | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| Chemi-Lumi One L: Luminol 50 mL, Peroxide 50 mL (Sufficient substrate for 800 cm² of blotting membrane) |
4°C | 07880-54 | 2 x 50 ml | 92.00 | Buy |
| Chemi-Lumi One L: Luminol 250 mL, Peroxide 250 mL (Sufficient substrate for 4,000 cm² of blotting membrane) |
4°C | 07880-70 | 1 kit | 220.00 | Buy |