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Chemi-Lumi One L: Chemiluminescent Reagents for Western Blotting

Picogram Sensitivity Substrate for HRP / Economical - costs much less than other ECL substrate


  • Picogram sensitivity - better signal than GE ECL Substrate
  • Economical - costs much less than other ECL substrates
  • Long light emission

Comparison of sensitivity and duration

Chemi-Lumi One L has more sensitive and longer signal duration than GE ECL Substrate. Western blot of identical biotinylated samples probed with respective streptavidin-HRP conjugates. The membranes were incubated with substrate that was prepared according to the manufacturers' instructions.
Lane1: 1: 50 dilution Lane3: 1: 200
Lane2: 1: 100 dilution Lane4: 1: 400

Comparison of price

CompanyNacalai USA, Inc.GE Healthcare Life Science
Item Chemi-Lumi One L Amersham ECL™
Price $190 $290
Cat. No. 07880-70 GERPN2106
Quantity For 4,000 cm² membrane For 4,000 cm² membrane


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  2. Chihara, Yusuke, et al. "Histone deacetylase inhibitor OBP‑801 and amrubicin synergistically inhibit the growth of squamous cell lung carcinoma by inducing mitochondrial ASK1‑dependent apoptosis." International Journal of Oncology 56.3 (2020): 848-856.
  3. Sheikh, Amin Alborzian Deh, Chizuru Akatsu, and Takeshi Tsubata. "Identification of Siglec Cis-Ligands by Proximity Labeling." Lectin Purification and Analysis. Humana, New York, NY, 2020. 75-83.
  4. Roudkenar, Mehryar Habibi, et al. "Disturbance in the regulation of miR 17-92 cluster on HIF-1-α expression contributes to clinically relevant radioresistant cells: an in vitro study." Cytotechnology (2020): 1-13.
  5. Umekawa, Midori, et al. "Mitotic cyclin Clb4 is required for the intracellular adaptation to glucose starvation in Saccharomyces cerevisiae." FEBS letters 594.8 (2020): 1329-1338.
  6. Norikura, Toshio, et al. "cis-Banglene, a bangle (Zingiber purpureum)-derived bioactive compound, promotes mitochondrial biogenesis and glucose uptake by activating the IL-6/AMPK signaling pathway in C2C12 skeletal muscle cells." Journal of Functional Foods 64 (2020): 103632.
  7. Kasahara, Takaoki, et al. "Cardiolipin is essential for early embryonic viability and mitochondrial integrity of neurons in mammals." The FASEB Journal (2020).
  8. Uehara, Natsumi, et al. "Oncolytic effect of Midkine promoter–based conditionally replicating adenoviruses expressing EGFR siRNA in head and neck squamous cancer cell line T891." Cancer Reports.

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Chemi-Lumi One L (Luminol 250 mL, Peroxide 250 mL, Sufficient substrate for 4,000 cm² of blotting membrane) 07880-70 4°C 1 kit 190.00 Buy